LOCUS       V001826                 6405 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001826
VERSION     V001826
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Kok RG, Young DM, Ornston LN.
  TITLE     Phenotypic expression of PCR-generated random mutations in a
            Pseudomonas putida gene after its introduction into an Acinetobacter
            chromosome by natural transformation
  JOURNAL   Appl. Environ. Microbiol. 65 (4), 1675-1680 (1999)
   PUBMED   10103267
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Young DM, Kok RG, Ornston L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2000) Molecular, Cellular, and Developmental
            Biology, Yale University, P.O. Box 208103 Room KBT 840, New Haven,
            CT 06520, USA
REFERENCE   3  (bases 1 to 6405)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6405)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "1999"; volume: "65"; issue: "4"; pages:
            "1675-1680"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-JUN-2000) Molecular, Cellular, and Developmental Biology, Yale
            University, P.O. Box 208103 Room KBT 840, New Haven, CT 06520, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             571..1599
                     /gene="lifO"
                     /label="Lipase chaperone"
                     /note="Lipase chaperone from Acinetobacter baylyi (strain
                     ATCC 33305 / BD413 / ADP1). Accession#: Q43961"
     promoter        1749..1777
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    1929..1985
                     /label="polylinker"
                     /note="polylinker"
     regulatory      2007..2012
                     /label="PaphA3"
                     /note="PaphA3"
                     /regulatory_class="promoter"
     CDS             2069..2860
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2929..3096
                     /label="truncated lipA"
                     /note="truncated lipA"
     rep_origin      complement(4568..5156)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5330..6187)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6188..6292)
                     /label="AmpR promoter"
ORIGIN
        1 tgatgattat cagccagcag agaattaagg aaaacagaca ggtttattga gcgcttatct
       61 ttccctttat ttttgctgcg gtaagtcgca taaaaaccat tcttcataat tcaatccatt
      121 tactatgtta tgttctgagg ggagtgaaaa ttcccctaat tcgatgaaga ttcttgctca
      181 attgttatca gctatgcgcc gaccagaaca ccttgccgat cagccaaacg tctcttcagg
      241 ccactgacta gcgataactt tccccacaac ggaacaactc tcattgcatg ggatcattgg
      301 gtactgtggg tttagtggtt gtaaaaacac ctgaccgcta tccctgatca gtttcttgaa
      361 ggtaaactca tcacccccaa gtctggctat gcagaaatcc ccatcttgct ggattttgca
      421 gtcataatgt gctctacatc aaataaatta aatataatgt taaaaaattc attcatccgt
      481 ttttgtatta tttgtccaaa gttatgctat ttttataatc attcaattca atgatgaaat
      541 aaaaattgcg tcgtattgga atataaaaac atgaatggat cgaaaaaaat ttacctaggg
      601 attgggttag tcgcattatt aatgatattc atctattggt taatgccaaa agatactgcc
      661 aatgcatcct ctcaaattga gagtacgaat gcctcagcaa ttatagcaac cagtccaggg
      721 cagcagaatc aactttctga aaatacgaca ccatttggta gtgtaagtca gcatgatact
      781 caagtgaatt gccaacttca gctcaatgca gctaaccact taatcgtcaa tgaacaaaca
      841 cgtaactgct ttgaatattt tcttacgcaa tacggcgaga aaagcctgac tcaaattgat
      901 caagacatca aaaattactt cactcaaagt cttccccagc ctgcacgcga tcaggcacaa
      961 gatttatggc aacgttatct caaatatcgc gaagaactcg gaaatttaaa agaaccagcg
     1021 atcgccaaaa ctgatattgc ttattatcgt gcagtattta cctctagaca gatgctcaga
     1081 caacgttttt tttctgcgac agaaattgca gggctgttcg gctcagaaga tatttataac
     1141 caatataccc tggaacggat ggcgattcta aataatagca aactcagtga gattgaaaaa
     1201 gccaagcaac tcaaagcatt atttgatcaa ttgccccaag actggaaagc gaatcttgaa
     1261 caactttcaa aattggatga tttaaagcaa ttaaccacat caatcaaaaa gaatggaggc
     1321 tcagcacaag agttacatga tatgcgaacc aatttggttg gacatgatgc cactgcacgt
     1381 ctagaacaat tggatgtcga acgctcaaac tggaaaagta acgtcactca gtatcttgat
     1441 gagcgtcaga ctattttaaa cagcaatatg agtgatacag cgaaacagaa cgccatcagt
     1501 gcacttagaa gtaaaaattt tactgcacca caggtacaga ttcgggtaca agcatttgaa
     1561 tctgcaaaag atcaaggaca aagcttaccc ttttctgaat aattttatat cactatttaa
     1621 tcaaggagaa aatagacctt aatttagctt tacccgtgtt ttaacggaaa taatatgcgg
     1681 tttgaaaatg gatgaatttt caaactcaga aaaatgaaat ttagggaaat aaaatggaat
     1741 tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc acagttaaat
     1801 tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc
     1861 accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg
     1921 cgggatgggg atcctctaga gtcgacgtca cgcgtccatg gagatctcga ggcctgcagg
     1981 catgcaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
     2041 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
     2101 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
     2161 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
     2221 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
     2281 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
     2341 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
     2401 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
     2461 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
     2521 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
     2581 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     2641 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     2701 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     2761 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     2821 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     2881 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaggt accttcatgt
     2941 agcagtcact tgggtacagt aattcgagat aattacgtat ggaatcatct ggatgaagtc
     3001 aatcaaattc tgggtttcga tctatttttg cacaagaccc cgtatccatc tttagacaac
     3061 atgccaatcg tctcaaaggt caaaatctat aattaagatg caccagatgt aaaaaatgcg
     3121 cccaatcagg cgcattttta tgctgataag cgaagttaaa attaacctcg tttacgcgct
     3181 ggcaattttt cacgaatacg cgcagcttta ccagacaact cacgaaggta gtaaagttta
     3241 gcacgacgca cgtcaccacg acgtttcact tcaattttag caacaactgg tgagtgagtt
     3301 ggaaacacgc tcaacaccaa cacagctaga aatttacgta ctgtaaacgc agagtttaaa
     3361 ccacgatttt cttcgcaata acaacacctt caaatgcttg aagacgctca cgatcaccct
     3421 cttttacttt aacttggaca atcactgtgt caccaggagc aaaagcaggg atatcagatt
     3481 ttaactgtgc attttctaca gcttgaacta aaggatgttt accactcatt gtggaatctc
     3541 cagtatgtgc gggagagaag aggtctctta caccgctggg gacagaggct aaaacgcata
     3601 tcaacccgtt taactttccc tctattactt atactcaagg tataaaaata aagagccata
     3661 tatcagatca ctcaattttt tgagtcatct aaaaatgtta gccattttct ttgctgcttg
     3721 gtcaacgtca ctttttcaac cagttcaggt cgaccgatgc ccttgagagc cttcaaccca
     3781 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     3841 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     3901 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     3961 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     4021 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
     4081 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
     4141 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
     4201 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac tgcgatttat
     4261 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
     4321 gtctgcctcc ccgcgttgcg gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     4381 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
     4441 cggagaactg tgaatgcgca aaccaaccct tggcaggaac atatccatcg cgtccgccat
     4501 ctccagcagc cgcacgcggc gcatctcggg cagcgttgaa ccgtaaaaag gccgcgttgc
     4561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4741 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     4801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4981 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5641 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5881 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5941 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6001 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6061 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6361 ataggcgtat cacgaggccc tttcgtcttc aagaattgct gcagg
//
