LOCUS       V001831                 3156 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001831
VERSION     V001831
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE.
  TITLE     An enzyme-coupled biosensor enables (S)-reticuline production in
            yeast from glucose
  JOURNAL   Nat. Chem. Biol. 11 (7), 465-471 (2015)
   PUBMED   25984720
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJJ., Dueber
            JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA
REFERENCE   3  (bases 1 to 3156)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3156)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2015"; volume: "11"; issue: "7"; pages: "465-471"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-APR-2015) Department of Bioengineering, University of
            California, Berkeley, 2151 Berkeley Way Room 542, Berkeley, CA
            94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..8
                     /label="BsaI recognition site"
                     /note="BsaI recognition site"
     CDS             11..1501
                     /gene="CYP76AD1"
                     /label="Cytochrome P450 76AD1"
                     /note="Cytochrome P450 76AD1 from Beta vulgaris.
                     Accession#: I3PFJ5"
     misc_feature    complement(1504..1507)
                     /label="BsaI recognition site"
                     /note="BsaI recognition site"
     misc_feature    complement(1509..1514)
                     /label="BsaI recognition site"
                     /note="BsaI recognition site"
     terminator      complement(1521..1555)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1629..2285)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2286..2389)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      2467..3055
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tcggtctcct atggatcatg caacattagc tatgatatta gcaatttggt ttatttcatt
       61 tcattttatt aaattattat tctcacaaca aacaacaaaa ttgttacctc caggtcctaa
      121 acctttacca attattggta atatcttgga agtaggaaag aagcctcata gatcatttgc
      181 aaatttggct aagatccacg gtcctttaat ctctttgagg ttaggatcag ttaccaccat
      241 tgttgtatca tctgctgatg tcgccaaaga gatgttcttg aagaaggatc accctttatc
      301 aaacaggaca attccaaatt cagtaactgc tggtgaccac cacaaattga ctatgtcttg
      361 gttgcctgtt tcacctaaat ggagaaactt cagaaagata actgctgttc atttattgtc
      421 tccacaaaga ttagatgcat gccagacctt tagacatgca aaggttcagc aattatatga
      481 atacgttcaa gagtgcgctc agaagggtca ggcagttgac attggtaaag ctgccttcac
      541 aacttctttg aatttattgt ctaaattatt cttttctgtt gaattggccc atcataaatc
      601 acatacctct caagagttta aggaattaat ttggaatatt atggaagaca tcggtaaacc
      661 aaactacgcc gattacttcc caattttagg ttgcgtcgat ccatcaggaa ttagaagaag
      721 gttggcatgt tcttttgata agttaattgc agtatttcaa ggaataatat gtgagaggtt
      781 agctccagat tcatcaacta caactactac aactacagat gatgtcttgg acgtattatt
      841 gcaattgttt aagcagaatg aattaactat gggagagatt aaccacttgt tagtcgatat
      901 tttcgatgcc ggtactgata ctacatcatc tacctttgag tgggttatga ctgagttaat
      961 aagaaaccct gaaatgatgg aaaaggccca agaggaaata aaacaggtat tgggtaagga
     1021 taagcaaatc caagagtcag acataataaa tttgccatac ttgcaagcaa tcattaagga
     1081 aactttgagg ttgcacccac ctacagtttt cttgttgcct agaaaagccg acacagacgt
     1141 agaattgtac ggatacattg ttccaaagga tgctcaaatt ttggtaaact tgtgggccat
     1201 aggtagagat ccaaatgctt ggcaaaatgc cgatattttc tcacctgaaa gatttatagg
     1261 ttgtgagatt gatgtaaagg gaagagattt tggtttattg ccttttggtg ctggtagaag
     1321 aatttgccct ggtatgaatt tggccataag gatgttgaca ttgatgttgg ccacattatt
     1381 gcaattcttc aactggaaat tagagggaga catttcacct aaagatttgg acatggatga
     1441 aaagtttggt atagccttgc agaaaactaa acctttaaaa ttgatcccaa ttccaagata
     1501 cggatcctga gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1561 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1621 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1681 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1741 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1801 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac
     1861 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1921 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1981 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     2041 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     2101 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     2161 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2221 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2281 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     2341 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa
     2401 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2461 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2521 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2581 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2641 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2701 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2761 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2821 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2881 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2941 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3001 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3061 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3121 tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
