LOCUS       40924_48518        3231 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning T vector pZKT19, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Zheng D, Liu X, Zhou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2007) Lab of Genetics and Molecular Biology, 
            Northeast Forestry University, P.O. 311, 26 Hexing Road, Harbin, HL 
            150040, China
REFERENCE   2  (bases 1 to 3231)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2007) Lab of Genetics and Molecular Biology, Northeast 
            Forestry University, P.O. 311, 26 Hexing Road, Harbin, HL 150040, 
            China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(167..883)
                     /codon_start=1
                     /label=KillerRed
                     /note="red fluorescent protein KillerRed, a
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /translation="MGSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLA
                     FIGFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
     primer_bind     934..950
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1010..1026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1034..1050)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1058..1088)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1103..1124)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1412..2000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2174..3031)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3032..3136)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcatcaatcc tcgtcgctac
      181 cgatggcgct ggtgatgcgg ggcacgctgt gggcgtaggc cacctcgcgc tggcacacgt
      241 ggtcgcgctt gtcgctggtg tccctcatct gcttggtgat gatggtcacg aagtgtgggc
      301 cggggatctc gatggcgcgg ctgccgttga aggtcatctt gctgtcgaag tggcccatca
      361 tcaggccgcc gtcggcggtg gtgaagccga tgaaggccag ctggcgcacg gcgttggggc
      421 cgtgggggaa catgtgggtc tcgttgggca ggatgtccac cagctggtcg cgcatgatgg
      481 ggccgtcggg ctggaagccg tcgcagttca cggtgatgcg gctcaccacg caggtgtcgt
      541 ccagctcgta ggtgtggtgg ctggtcatgg tgccgtcgtt ctcgaagcgc acggtgcggt
      601 cgatgctcag gccctcgggg aagcactcct gggcgaaatg gctgatgccg tcggggtagc
      661 gggcgaagaa gggctcgccg tactggatca ggtggcagat gggcttccag ctcatgggca
      721 gcttgccggt ctcgcacacg gcgtgcacgt tgaagtcgcc gtgggggaac ttgctgctgc
      781 cgtcggccac gatggtgaac ttctggccgt tcacctcgcc gtcgatgaag attttgaagg
      841 tcatgtcgct ctggaacagg gcggggccgc cctctgaacc cattgcaagg cgattaagtt
      901 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc
      961 tcggtacccg gggatccaag cttatgagtg gaagcttggc gtaatcatgg tcatagctgt
     1021 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1081 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1141 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1201 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1261 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1321 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1381 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1441 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1501 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1561 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1621 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1681 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1741 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1801 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1861 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1921 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1981 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2041 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2101 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2161 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2221 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2281 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2341 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2401 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2461 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2521 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2581 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2641 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2701 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2761 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2821 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2881 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2941 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3001 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3061 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3121 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     3181 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
