LOCUS       40924_48513        3241 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZKG19, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Zheng D, Liu X, Zhou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2007) Lab of Genetics and Molecular Biology, 
            Northeast Forestry University, P.O. 311, 26 Hexing Road, Harbin, HL 
            150040, China
REFERENCE   2  (bases 1 to 3241)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3241)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-JAN-2007) Lab of Genetics and Molecular Biology, Northeast 
            Forestry University, P.O. 311, 26 Hexing Road, Harbin, HL 150040, 
            China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(167..883)
                     /codon_start=1
                     /label=KillerRed
                     /note="red fluorescent protein KillerRed, a
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /translation="MGSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLA
                     FIGFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
     primer_bind     934..950
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    951..1007
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1020..1036)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1044..1060)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1068..1098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1113..1134)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1422..2010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2184..3041)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3042..3146)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcatcaatcc tcgtcgctac
      181 cgatggcgct ggtgatgcgg ggcacgctgt gggcgtaggc cacctcgcgc tggcacacgt
      241 ggtcgcgctt gtcgctggtg tccctcatct gcttggtgat gatggtcacg aagtgtgggc
      301 cggggatctc gatggcgcgg ctgccgttga aggtcatctt gctgtcgaag tggcccatca
      361 tcaggccgcc gtcggcggtg gtgaagccga tgaaggccag ctggcgcacg gcgttggggc
      421 cgtgggggaa catgtgggtc tcgttgggca ggatgtccac cagctggtcg cgcatgatgg
      481 ggccgtcggg ctggaagccg tcgcagttca cggtgatgcg gctcaccacg caggtgtcgt
      541 ccagctcgta ggtgtggtgg ctggtcatgg tgccgtcgtt ctcgaagcgc acggtgcggt
      601 cgatgctcag gccctcgggg aagcactcct gggcgaaatg gctgatgccg tcggggtagc
      661 gggcgaagaa gggctcgccg tactggatca ggtggcagat gggcttccag ctcatgggca
      721 gcttgccggt ctcgcacacg gcgtgcacgt tgaagtcgcc gtgggggaac ttgctgctgc
      781 cgtcggccac gatggtgaac ttctggccgt tcacctcgcc gtcgatgaag attttgaagg
      841 tcatgtcgct ctggaacagg gcggggccgc cctctgaacc cattgcaagg cgattaagtt
      901 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc
      961 tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg
     1021 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1081 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1141 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1201 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1261 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1381 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1441 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1501 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1561 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1621 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1681 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1741 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1801 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1861 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1921 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1981 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2461 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     2581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     2641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     2701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     2761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     2821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     2881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     2941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3061 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3121 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3181 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     3241 c
//
