LOCUS       40924_48508        3386 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZK18S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Liu X, Liu X, Zou D, Shi R, Li Z, Zheng D.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2010) Northeast Forestry University, Laboratory of
            Genetics and Molecular Biology, Mailbox 311, 26 Hexing Road, Harbin,
            Heilongjiang 150040, China
REFERENCE   2  (bases 1 to 3386)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3386)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (29-APR-2010) Northeast Forestry University, Laboratory of Genetics 
            and Molecular Biology, Mailbox 311, 26 Hexing Road, Harbin, 
            Heilongjiang 150040, China"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(408..1124)
                     /codon_start=1
                     /label=KillerRed
                     /note="red fluorescent protein KillerRed, a
                     genetically-encoded photosensitizer (Bulina et al., 2006)"
                     /translation="MGSEGGPALFQSDMTFKIFIDGEVNGQKFTIVADGSSKFPHGDFN
                     VHAVCETGKLPMSWKPICHLIQYGEPFFARYPDGISHFAQECFPEGLSIDRTVRFENDG
                     TMTSHHTYELDDTCVVSRITVNCDGFQPDGPIMRDQLVDILPNETHMFPHGPNAVRQLA
                     FIGFTTADGGLMMGHFDSKMTFNGSRAIEIPGPHFVTIITKQMRDTSDKRDHVCQREVA
                     YAHSVPRITSAIGSDED"
     misc_feature    complement(1125..1150)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1165..1181)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1189..1205)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1213..1243)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1258..1279)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1567..2155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2329..3186)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3187..3291)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttcaatc ctcgtcgcta
      421 ccgatggcgc tggtgatgcg gggcacgctg tgggcgtagg ccacctcgcg ctggcacacg
      481 tggtcgcgct tgtcgctggt gtccctcatc tgcttggtga tgatggtcac gaagtgtggg
      541 ccggggatct cgatggcgcg gctgccgttg aaggtcatct tgctgtcgaa gtggcccatc
      601 atcaggccgc cgtcggcggt ggtgaagccg atgaaggcca gctggcgcac ggcgttgggg
      661 ccgtggggga acatgtgggt ctcgttgggc aggatgtcca ccagctggtc gcgcatgatg
      721 gggccgtcgg gctggaagcc gtcgcagttc acggtgatgc ggctcaccac gcaggtgtcg
      781 tccagctcgt aggtgtggtg gctggtcatg gtgccgtcgt tctcgaagcg cacggtgcgg
      841 tcgatgctca ggccctcggg gaagcactcc tgggcgaaat ggctgatgcc gtcggggtag
      901 cgggcgaaga agggctcgcc gtactggatc aggtggcaga tgggcttcca gctcatgggc
      961 agcttgccgg tctcgcacac ggcgtgcacg ttgaagtcgc cgtgggggaa cttgctgctg
     1021 ccgtcggcca cgatggtgaa cttctggccg ttcacctcgc cgtcgatgaa gattttgaag
     1081 gtcatgtcgc tctggaacag ggcggggccg ccctctgaac ccatggatcc caagcttccc
     1141 atggcatgcg aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1201 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     1261 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     1321 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     1381 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1441 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1501 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1561 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1621 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1681 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1741 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1801 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1861 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1921 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1981 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2041 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2101 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2161 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2221 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2281 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2341 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2401 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2461 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2521 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2581 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2641 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2701 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2761 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2821 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2881 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2941 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3001 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3061 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3301 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3361 taggcgtatc acgaggccct ttcgtc
//
