LOCUS       40924_48503        6740 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pZJY41, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Zhao JY, Zhong L, Shen MJ, Xia ZJ, Cheng QX, Sun X, Zhao GP, Li YZ, 
            Qin ZJ.
  TITLE     Discovery of the autonomously replicating plasmid pMF1 from 
            Myxococcus fulvus and development of a gene cloning system in 
            Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 74 (7), 1980-1987 (2008)
  PUBMED    18245244
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Zhao J, Zhong L, Qin Z, Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) State Key Laboratory of Microbial 
            Technology, School of Life Science, Shandong University, 27 South 
            Road of Shanda, Jinan, Shandong 250100, People's Republic of China
REFERENCE   3  (bases 1 to 6740)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "7"; pages:
            "1980-1987"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2007) State Key Laboratory of Microbial Technology, School 
            of Life Science, Shandong University, 27 South Road of Shanda, 
            Jinan, Shandong 250100, People's Republic of China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             299..1087
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2114..4018
                     /codon_start=1
                     /gene="rep-pMF1"
                     /product="putative Rep-pMF1 protein"
                     /label=rep-pMF1
                     /protein_id="ABY83639.1"
                     /translation="MKRRALTSQPTPARALVHGAGSGERPTLREPSRAKSQPRNRRSGL
                     DVEPSAASASSLPADQVAQLRASTARLRREQSQRQRRVNAGKARRNEERKWAALELATG
                     TALPLVDTVGFRPAEVSKPETYVTLEDTHGLEGLARGERAEQELEAFRASHPSRQVAPP
                     AALAIAAILYSTRKAEGGADARDCASLGWQVPVRGWCEMTGYRERAVQAAFAWLADEGL
                     VRRFGDYVTVKHLTRLRPAGERLDLWLDAKGKARRWVQVYSVTYLTNAGAAWLERAGFD
                     LAETRGHGVAWRRRGFLARVGRALAGLWRTVRRRLGGGGSVAVGAGATCTPYPAKQVEV
                     CSRRGDDSQPTASLEELIGAESPPHGGDVTGSASAKPDPRAANAEHARVEGAPAQGGHA
                     SMGSASAKLEGSKPPPSALGPEWEKKLGLTAEGIAAGLAWEGGGLLAYAEVVDRCWTRT
                     TTTTRAAPSDVPSGSRNMFGRRRVWRCRGCGAESRYKGATVRHAQGCTAVGAWHLARGP
                     AVETWSPAMTAELAWPEDWEAHPELRPRLRRLFVPVAAELTRRIQARAAEARRAAEAEE
                     AERRHLEVWMSKHPECGGAFRAPVYDSSVCVADGPPNAWGRAPLCYCDACSQKRRDIE"
     gene            2114..4018
                     /gene="rep-pMF1"
                     /label=rep-pMF1
     promoter        complement(4378..4396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4654..5242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5416..6273)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6274..6378)
                     /label=AmpR promoter
     promoter        6724..6740
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaactcgagc agctgaagct tgtgctgacc ccgggtgaat gtcagctact gggctatctg
       61 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
      121 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
      181 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
      241 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
      301 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      361 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      421 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
      481 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      541 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      601 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      661 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      721 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      781 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
      841 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      901 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      961 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     1021 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1081 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1141 tcacgagatt tcgatcccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1201 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1261 cccgggctcg ataagcttgc atgcctgcag gtcgacggga agtgccccaa ggacaagtgg
     1321 gagtggcgcg ggggctgcta cgtccctgtc ctcgtgggcg cgcgccaggc cgttcgcccg
     1381 tagagtgagc agaaatcgtt gagtttttgg ctggcctcaa gcaggctggc gagcaaatgt
     1441 tctgagaggg ccgttttgtc cggctccggc cctcgttggg cacgtcctga gtgttcgcag
     1501 cgctctggga cgtgttatag ctgtggacgc gttccatccg aggactcctt agccggacgc
     1561 ctcgcagaac gcgcttagag gggcccgttg gcgcgtgctg cgaacacgcg ccggtggcct
     1621 ctcgtttttg gtggtccgtg gacgactttc gcagagtcgt ggtcgcggtt gacggtagtg
     1681 acgttggtga gcgcgaggcg ctgaaagccc gctggctcca gtacgcgcta gccgtgatgc
     1741 cgttcgatgt ccgggtcgcg gtggcctacc aggccgtgcg ggagggcgag gatgcactcc
     1801 gcgaggcggg ccggctgcgc gagcaggcgt gcgccgacgc gaaagccctg gctgacctcc
     1861 gtggtgatgt cgcggagctg cgcgagcaga acgcggagct acgcgagcgg aacgcggagc
     1921 tgttccgcac ggcggtgact gctcagcgcc ggctccgcga gctgcggccc ggtgactccg
     1981 ggttctcgtt tggccgggat ggtgcaccgc ctgttcgcca cgtcgcgaac gaggagcggt
     2041 ccgccctggt gcctgtcgtc atggagaccc cctggccccc cgcgcccatt gagggcgagg
     2101 acggccaggc ggagtgaagc gccgagcgct gacatcgcag ccgacgcccg cgcgggcgct
     2161 cgtgcacgga gccgggagcg gagagcgccc gactctgcgc gagccgtcgc gcgcgaagag
     2221 ccaaccgcgg aatcggcgct ctgggctgga tgtggagccg agtgccgcta gcgcgtcgtc
     2281 actccccgct gaccaggtgg ctcagctccg cgcctcaacc gcgcgactcc gccgcgagca
     2341 gtcgcagcgc cagcgccgcg tgaacgcggg caaggcccgg cgcaatgagg agcggaagtg
     2401 ggccgcgctc gagctggcga cagggacggc gctccccctg gtcgatacgg tcggcttccg
     2461 gccggccgag gtgagcaagc ccgagacgta cgtcacgctc gaggacacgc acgggctcga
     2521 gggcctggcg cgcggcgagc gcgcagagca ggagctggag gcgttccgcg cgagccatcc
     2581 gtcgcgccag gtcgcgcccc cggccgcgct ggcgatcgct gcgatcctct actcgacgcg
     2641 caaggcggag ggtggtgcgg acgcgcggga ctgtgcctct cttggatggc aagtcccggt
     2701 ccgtggatgg tgcgaaatga cgggctaccg cgagcgggcc gtgcaggccg cgttcgcgtg
     2761 gctcgctgac gaggggctcg tcagacgatt cggggactac gtcacggtca agcacctcac
     2821 ccggctgcga cctgctggcg agcgtctgga tctctggctc gacgccaagg gcaaagcacg
     2881 ccgctgggtg caggtctaca gcgtgactta cctgaccaac gctggcgcgg cctggctcga
     2941 gcgcgcgggc ttcgacctgg ccgagaccag gggccacggc gttgcgtggc gccgtagggg
     3001 cttcctggcc cgtgtgggcc gtgccctggc cggcctgtgg agaaccgtgc ggcggaggtt
     3061 gggcggtggc ggctctgttg ccgtaggcgc cggggctacg tgcacccctt accctgcgaa
     3121 gcaggtagaa gtctgttcaa ggcgtggtga cgactcccag cccactgcct ccctcgagga
     3181 gctgataggg gcggagtccc ccccccacgg gggggacgtc actggaagcg catcagccaa
     3241 gcccgaccca cgggccgcaa acgccgagca cgctcgggtg gagggcgcgc ccgctcaagg
     3301 cgggcacgcc agcatgggga gcgcgagcgc caagctcgaa ggcagcaagc cccctccctc
     3361 ggcactgggg ccggagtggg agaagaagct gggcctcacc gccgaaggca ttgccgctgg
     3421 gctggcctgg gaggggggcg gcctgctcgc ttacgccgag gtcgtggacc ggtgctggac
     3481 caggacgacg acgacgacgc gggcggcccc ctcggacgtt ccgagcggct cccgcaacat
     3541 gttcgggagg cgccgcgtgt ggcggtgccg tggctgtggt gccgagtccc gctacaaggg
     3601 cgccaccgtg cgccacgcgc aaggatgtac cgctgtgggg gcctggcacc tggcgcgggg
     3661 cccggccgtg gagacctggt ctcccgcgat gacggcggag ctggcctggc ccgaggactg
     3721 ggaggctcac ccggagctgc ggccgaggtt gcggcgcctg tttgttccgg tcgcggccga
     3781 gttgacgcgc cggattcaag ctcgagcggc ggaggcgcgc cgcgccgccg aagctgaaga
     3841 ggccgagcgc agacatctgg aagtctggat gtctaaacat ccagaatgcg gcggtgcgtt
     3901 ccgcgcgccg gtctatgact cgtcggtgtg cgtggcggat ggccctccga acgcgtgggg
     3961 ccgcgcgccg ctttgctact gtgatgcgtg cagtcagaag aggagggaca tcgaatgaaa
     4021 ccctgtagct gggaggctcg aggccggctt aatccccatc gcctagcgcg cggcgtgagc
     4081 gccttcaagc cgccggtcct ctctccgcac ggtgcgggcg cggggttgtc gcccgagctg
     4141 ccggaagagg cccgggactc gacgcggtgg cgggtggtcg tgcggttcgt gtcgctcaag
     4201 gaggcggcgc gaaaggcccg ggtggatgag tgcacaatgc gtcggtggtg cactcgcggt
     4261 tgggtgatgg ccgagcgcct ggcctccgga cacggcccgt ggattgttgc ggtcgactct
     4321 agaggatccc cgggtaccga gctcgaattc atcgatgata tcagatctgc cggtctccct
     4381 atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg
     4441 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4501 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4561 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4621 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4681 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4741 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4801 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4861 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4921 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4981 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5041 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5101 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5161 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5221 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5281 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5341 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5401 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5461 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5521 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5581 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5641 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5701 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5761 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5821 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5881 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5941 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6001 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6061 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6121 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6181 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6241 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6301 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6361 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     6421 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     6481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     6541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     6601 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     6661 ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
     6721 acgatttagg tgacactata
//
