LOCUS       40924_48498        5933 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Shuttle vector pZJY156, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Zhao JY, Zhong L, Shen MJ, Xia ZJ, Cheng QX, Sun X, Zhao GP, Li YZ, 
            Qin ZJ.
  TITLE     Discovery of the autonomously replicating plasmid pMF1 from 
            Myxococcus fulvus and development of a gene cloning system in 
            Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 74 (7), 1980-1987 (2008)
  PUBMED    18245244
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Zhao J, Zhong L, Qin Z, Li Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2007) State Key Laboratory of Microbial 
            Technology, School of Life Science, Shandong University, 27 South 
            Road of Shanda, Jinan, Shandong 250100, People's Republic of China
REFERENCE   3  (bases 1 to 5933)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5933)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "7"; pages:
            "1980-1987"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-DEC-2007) State Key Laboratory of Microbial Technology, School 
            of Life Science, Shandong University, 27 South Road of Shanda, 
            Jinan, Shandong 250100, People's Republic of China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             299..1087
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1429..3333
                     /codon_start=1
                     /gene="rep-pMF1"
                     /product="putative Rep-pMF1 protein"
                     /label=rep-pMF1
                     /protein_id="ABY83642.1"
                     /translation="MKRRALTSQPTPARALVHGAGSGERPTLREPSRAKSQPRNRRSGL
                     DVEPSAASASSLPADQVAQLRASTARLRREQSQRQRRVNAGKARRNEERKWAALELATG
                     TALPLVDTVGFRPAEVSKPETYVTLEDTHGLEGLARGERAEQELEAFRASHPSRQVAPP
                     AALAIAAILYSTRKAEGGADARDCASLGWQVPVRGWCEMTGYRERAVQAAFAWLADEGL
                     VRRFGDYVTVKHLTRLRPAGERLDLWLDAKGKARRWVQVYSVTYLTNAGAAWLERAGFD
                     LAETRGHGVAWRRRGFLARVGRALAGLWRTVRRRLGGGGSVAVGAGATCTPYPAKQVEV
                     CSRRGDDSQPTASLEELIGAESPPHGGDVTGSASAKPDPRAANAEHARVEGAPAQGGHA
                     SMGSASAKLEGSKPPPSALGPEWEKKLGLTAEGIAAGLAWEGGGLLAYAEVVDRCWTRT
                     TTTTRAAPSDVPSGSRNMFGRRRVWRCRGCGAESRYKGATVRHAQGCTAVGAWHLARGP
                     AVETWSPAMTAELAWPEDWEAHPELRPRLRRLFVPVAAELTRRIQARAAEARRAAEAEE
                     AERRHLEVWMSKHPECGGAFRAPVYDSSVCVADGPPNAWGRAPLCYCDACSQKRRDIE"
     gene            1429..3333
                     /gene="rep-pMF1"
                     /label=rep-pMF1
     promoter        complement(3571..3589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3847..4435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4609..5466)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5467..5571)
                     /label=AmpR promoter
     promoter        5917..5933
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaactcgagc agctgaagct tgtgctgacc ccgggtgaat atcagctact gggctatctg
       61 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
      121 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
      181 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
      241 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
      301 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      361 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      421 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
      481 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      541 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      601 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      661 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      721 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      781 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
      841 tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      901 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      961 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     1021 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1081 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1141 tcacgagatt tcgatcccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1201 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1261 cccgggctcg ataagcttgc atgcctgcag gtcgacgttc tcgtttggcc gggatggtgc
     1321 accgcctgtt cgccacgtcg cgaacgagga gcggtccgcc ctggtgcctg tcgtcatgga
     1381 gaccccctgg ccccccgcgc ccattgaggg cgaggacggc caggcggagt gaagcgccga
     1441 gcgctgacat cgcagccgac gcccgcgcgg gcgctcgtgc acggagccgg gagcggagag
     1501 cgcccgactc tgcgcgagcc gtcgcgcgcg aagagccaac cgcggaatcg gcgctctggg
     1561 ctggatgtgg agccgagtgc cgctagcgcg tcgtcactcc ccgctgacca ggtggctcag
     1621 ctccgcgcct caaccgcgcg actccgccgc gagcagtcgc agcgccagcg ccgcgtgaac
     1681 gcgggcaagg cccggcgcaa tgaggagcgg aagtgggccg cgctcgagct ggcgacaggg
     1741 acggcgctcc ccctggtcga tacggtcggc ttccggccgg ccgaggtgag caagcccgag
     1801 acgtacgtca cgctcgagga cacgcacggg ctcgagggcc tggcgcgcgg cgagcgcgca
     1861 gagcaggagc tggaggcgtt ccgcgcgagc catccgtcgc gccaggtcgc gcccccggcc
     1921 gcgctggcga tcgctgcgat cctctactcg acgcgcaagg cggagggtgg tgcggacgcg
     1981 cgggactgtg cctctcttgg atggcaagtc ccggtccgtg gatggtgcga aatgacgggc
     2041 taccgcgagc gggccgtgca ggccgcgttc gcgtggctcg ctgacgaggg gctcgtcaga
     2101 cgattcgggg actacgtcac ggtcaagcac ctcacccggc tgcgacctgc tggcgagcgt
     2161 ctggatctct ggctcgacgc caagggcaaa gcacgccgct gggtgcaggt ctacagcgtg
     2221 acttacctga ccaacgctgg cgcggcctgg ctcgagcgcg cgggcttcga cctggccgag
     2281 accaggggcc acggcgttgc gtggcgccgt aggggcttcc tggcccgtgt gggccgtgcc
     2341 ctggccggcc tgtggagaac cgtgcggcgg aggttgggcg gtggcggctc tgttgccgta
     2401 ggcgccgggg ctacgtgcac cccttaccct gcgaagcagg tagaagtctg ttcaaggcgt
     2461 ggtgacgact cccagcccac tgcctccctc gaggagctga taggggcgga gtcccccccc
     2521 cacggggggg acgtcactgg aagcgcatca gccaagcccg acccacgggc cgcaaacgcc
     2581 gagcacgctc gggtggaggg cgcgcccgct caaggcgggc acgccagcat ggggagcgcg
     2641 agcgccaagc tcgaaggcag caagccccct ccctcggcac tggggccgga gtgggagaag
     2701 aagctgggcc tcaccgccga aggcattgcc gctgggctgg cctgggaggg gggcggcctg
     2761 ctcgcttacg ccgaggtcgt ggaccggtgc tggaccagga cgacgacgac gacgcgggcg
     2821 gccccctcgg acgttccgag cggctcccgc aacatgttcg ggaggcgccg cgtgtggcgg
     2881 tgccgtggct gtggtgccga gtcccgctac aagggcgcca ccgtgcgcca cgcgcaagga
     2941 tgtaccgctg tgggggcctg gcacctggcg cggggcccgg ccgtggagac ctggtctccc
     3001 gcgatgacgg cggagctggc ctggcccgag gactgggagg ctcacccgga gctgcggccg
     3061 aggttgcggc gcctgtttgt tccggtcgcg gccgagttga cgcgccggat tcaagctcga
     3121 gcggcggagg cgcgccgcgc cgccgaagct gaagaggccg agcgcagaca tctggaagtc
     3181 tggatgtcta aacatccaga atgcggcggt gcgttccgcg cgccggtcta tgactcgtcg
     3241 gtgtgcgtgg cggatggccc tccgaacgcg tggggccgcg cgccgctttg ctactgtgat
     3301 gcgtgcagtc agaagaggag ggacatcgaa tgaaaccctg tagctgggag gctcgaggcc
     3361 ggcttaatcc ccatcgccta gcgcgcggcg tgagcgcctt caagccgccg gtcctctctc
     3421 cgcacggtgc gggcgcgggg ttgtcgcccg agctgccgga agaggcccgg gactcgacgc
     3481 ggtggcgggt ggtcgtgcgg tcgtgtcgac tctagaggat ccccgggtac cgagctcgaa
     3541 ttcatcgatg atatcagatc tgccggtctc cctatagtga gtcgtattaa tttcgataag
     3601 ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4261 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     5641 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5701 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5761 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5821 atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta
     5881 caattaatac ataaccttat gtatcataca catacgattt aggtgacact ata
//
