LOCUS       V001838                 5231 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001838
VERSION     V001838
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJ, Lo J, Lynd LR.
  TITLE     Physiological roles of pyruvate ferredoxin oxidoreductase and
            pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum
            JW/SL-YS485
  JOURNAL   Biotechnol Biofuels 8, 138 (2015)
   PUBMED   26379770
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Zhou J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2014) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5231)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5231)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 8, 138 (2015)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-OCT-2014) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..741
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     misc_feature    802..1704
                     /label="upstream of Tsac_0628"
                     /note="upstream of Tsac_0628"
     rep_origin      complement(1947..2535)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2709..3566)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3567..3671)
                     /label="AmpR promoter"
     misc_feature    3765..4680
                     /label="downstream of Tsac_0629"
                     /note="downstream of Tsac_0629"
     regulatory      4688..5231
                     /label="kanamycin promoter from pMU433"
                     /note="kanamycin promoter from pMU433"
                     /regulatory_class="promoter"
ORIGIN
        1 gatcccatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc
       61 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
      121 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
      181 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact
      241 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
      301 gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat
      361 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
      421 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca
      481 gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat
      541 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc
      601 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc
      661 aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat
      721 ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca
      781 cgttactaaa gggaattcca gtatcatggg gcttttcctt tgaattaatt tgataaaggg
      841 tgtaaaatta tcatgtgatg atgtgatttt ggaggtaatc gcatgaattt aaataagata
      901 aatagaaaca cgtactacat agataatcct acgaatattg gcgtttatgc ctataaaaat
      961 aaaaattgtc tattagtaga tactggtata aacaatggac aagcgagaaa gattgacaat
     1021 gtattagtag aaaatggatt acatcccaaa tatatcataa acacccacaa ccacatggac
     1081 cactgtggcg gtaatatata ctttaagact cagtaccctg gatgcgaaat ctatacatca
     1141 aataaggagc gattatatat agaaaatcca gagttaagag atattgtact attttcttca
     1201 tgcccaataa gagatcttga tacgactaat aaaacatttt ctgtagattt tgtccttgat
     1261 tatggtatct caaaaattgg cgatgaaaag tttgatataa tatctttggt ggggcattca
     1321 atagatcaaa tcggcgtcat tacacctgat agagtatgtt ttttgggaga cagtgtattt
     1381 agcgaggata caataaaaaa atattcactt ccatatttgt ttaatataga aaaaagcatt
     1441 gaaactttaa aaaagctaaa agaggtggat gcagactatt tcgtaataag tcacatagat
     1501 agagtgctta ataaggatga gcttgatgtc ctgatagaaa agaatatttc aaatatagaa
     1561 gataatgttg aaattatact ggaattattg gaacaacctc agacaaggga aggattattg
     1621 caaaacttag taatattaaa cgatctgcca ttaaatttta ctcagtatta tatttatttt
     1681 tcatcggttt ccgcattttt gagcctgatg catcattaat gaatcggcca acgcgcgggg
     1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3301 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3721 atgacattaa cctataaaaa taggcgtatc acgagatgca tcagttaatt ctcgccattc
     3781 cccttctttt aaattttcat ctaattttaa ttggcctatt gaaagccttt ttaagtagac
     3841 gacttttgag cctatcgctt caaacattct ttttatttga tgatatttac cttctctgat
     3901 tgagacatat actttcgatg tactgcctga agatattatt tccagttttg ccggcatagt
     3961 cttgtaacca tcgtctaata gtatgccatc tgaaaacaaa gatacgtcat cttcatcgat
     4021 aaaacccaaa acttctgcat agtatttttt gaaaacgtgt ttttttggcg ataagagctt
     4081 atgagatagt tcaccgtcat ttgtaattaa aagtaatccc tctgtgtctt tatcaagcct
     4141 tcctgctggg aaaacctttc ttgcctttat atggtgtggc aaaagatcta caacggtttt
     4201 ttctgatgga tcatatgttg cacagattac acctttcggt ttattcatca ttatatatat
     4261 gtattctttg tacgatattt tttcacttct aaacgtgatt atatctttat caggttgtac
     4321 tgcaaaaccg gggtcgtcaa tcgtcacatt atttattgca acaaggcctt cttttataaa
     4381 atttttaatt tcttttcttg tgccataacc catatttgat aaaagcttat ctattctcat
     4441 ttttgacatc ttaaattcct cctaaacaat atgactgtgc ttcttagtaa attatatccc
     4501 aaaaatataa aatttgtagc aaaaatgtga tatatatcat attttttgcg ttttcctgat
     4561 gatacaatta agatgatgtt tcaagataat aaattttctg aagtgtatac agtatattga
     4621 ctacaaagaa caaaatagct ataataaaac cataataaat cgttaggggg aataaatatg
     4681 ctgtgcaggt cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt
     4741 atgaaaacga gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct
     4801 accaagacga agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata
     4861 ctgataagat aatatatctt ttatatagaa gatatcgccg tatgtaagga tttcaggggg
     4921 caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg
     4981 catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat
     5041 tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttca aaacaacttt
     5101 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     5161 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     5221 aataaatggc g
//
