LOCUS       V001839                 3412 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001839
VERSION     V001839
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Zhou J, Olson DG, Lanahan AA, Tian L, Murphy SJ, Lo J, Lynd LR.
  TITLE     Physiological roles of pyruvate ferredoxin oxidoreductase and
            pyruvate formate-lyase in Thermoanaerobacterium saccharolyticum
            JW/SL-YS485
  JOURNAL   Biotechnol Biofuels 8, 138 (2015)
   PUBMED   26379770
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Zhou J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2014) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 3412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 8, 138 (2015)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-OCT-2014) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7..741
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     rep_origin      complement(1044..1632)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1806..2663)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2664..2768)
                     /label="AmpR promoter"
     regulatory      2869..3412
                     /label="kanamycin promoter from pMU433"
                     /note="kanamycin promoter from pMU433"
                     /regulatory_class="promoter"
ORIGIN
        1 gatcccatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc
       61 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
      121 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
      181 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact
      241 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
      301 gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat
      361 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
      421 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca
      481 gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat
      541 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc
      601 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc
      661 aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat
      721 ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca
      781 cgttactaaa gggaattcca gctgatgcat cattaatgaa tcggccaacg cgcggggaga
      841 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      901 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      961 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1021 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1081 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1141 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1201 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1261 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1321 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1381 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1441 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     1501 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1561 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1621 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1681 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1741 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1801 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1861 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1921 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     1981 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2041 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2101 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2161 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2221 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2281 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2341 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2401 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2461 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2521 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2581 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2641 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2701 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2761 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2821 acattaacct ataaaaatag gcgtatcacg agatgcatca gctgtgcagg tcgataaacc
     2881 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     2941 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     3001 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     3061 tttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc
     3121 ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa
     3181 cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct
     3241 ccgtcgatac tatgttatac gccaactttc aaaacaactt tgaaaaagct gttttctggt
     3301 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
     3361 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg cg
//
