LOCUS       V010344                 8304 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010344
VERSION     V010344
KEYWORDS    pZIP-mCMV-ZsGreen-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..404
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             411..668
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    715..840
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1337..1570
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1755..1799
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1948..1989
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2061..2178
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2219..2755
                     /label="mCMV promoter"
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2764..3456
                     /label="ZsGreen1"
                     /note="Zoanthus green fluorescent protein"
     misc_feature    3473..4055
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4054..4647
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     ncRNA           4671..4792
                     /label="5' miR-30a"
                     /note="sequence upstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     misc_RNA        4793..4861
                     /label="shRNA"
                     /note="shRNA"
                     /note="short hairpin RNA"
     gap             4796..4817
                     /estimated_length=22
     gap             4837..4858
                     /estimated_length=22
     ncRNA           4862..4988
                     /label="3' miR-30a"
                     /note="sequence downstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     misc_feature    5009..5597
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5805..6038
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        6134..6463
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(6552..7137)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7311..8168)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8169..8273)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatcctcg cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accagctagc gaggcgtggc
      421 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      481 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      541 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      601 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      661 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      721 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      781 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      841 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      901 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      961 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1021 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1081 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1141 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     1201 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     1261 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     1321 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     1381 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     1441 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     1501 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     1561 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     1621 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     1681 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     1741 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     1801 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1861 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1921 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1981 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     2041 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     2101 gaaagaatag tagacataat agcaacagac atacaaacta aagaactaca aaaacaaatt
     2161 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttatcgattg
     2221 gcacgtatac tgagtcatta gggactttcc aatgggtttt gcccagtaca taaggtcaat
     2281 aggggtgaat caacaggaaa gtcccattgg agccaagtac actgagtcaa tagggacttt
     2341 ccattgggtt ttgcccagta caaaaggtca atagggggtg agtcaatggg tttttcccat
     2401 tattggcacg tacataaggt caataggggt gagtcattgg gtttttccag ccaatttaat
     2461 taaaacgcca tgtactttcc caccattgac gtcaatgggc tattgaaact aatgcaacgt
     2521 gacctttaaa cggtactttc ccatagctga ttaatgggaa agtaccgttc tcagagccaa
     2581 tacacgtcaa tgggaagtga aagggcagcc aaaacgtaac accgccccgg ttttcccctg
     2641 gaaattccat attggcacgc attctattgg ctgagctgcg ttctacgtgg gtataagagg
     2701 cgcgaccagc gtcggtaccg tcgcagtctt cggtctgacc accgtagaac gcagaaccgg
     2761 tccatggccc agtccaagca cggcctgacc aaggagatga ccatgaagta ccgcatggag
     2821 ggctgcgtgg acggccacaa gttcgtgatc accggcgagg gcatcggcta ccccttcaag
     2881 ggcaagcagg ccatcaacct gtgcgtggtg gagggcggcc ccttgccctt cgccgaggac
     2941 atcttgtccg ccgccttcat gtacggcaac cgcgtgttca ccgagtaccc ccaggacatc
     3001 gtcgactact tcaagaactc ctgccccgcc ggctacacct gggaccgctc cttcctgttc
     3061 gaggacggcg ccgtgtgcat ctgcaacgcc gacatcaccg tgagcgtgga ggagaactgc
     3121 atgtaccacg agtccaagtt ctacggcgtg aacttccccg ccgacggccc cgtgatgaag
     3181 aagatgaccg acaactggga gccctcctgc gagaagatca tccccgtgcc caagcagggc
     3241 atcttgaagg gcgacgtgag catgtacctg ctgctgaagg acggtggccg cttgcgctgc
     3301 cagttcgaca ccgtgtacaa ggccaagtcc gtgccccgca agatgcccga ctggcacttc
     3361 atccagcaca agctgacccg cgaggaccgc agcgacgcca agaaccagaa gtggcacctg
     3421 accgagcacg ccatcgcctc cggctccgcc ttgccctgag aattcaattc cgcccctctc
     3481 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     3541 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     3601 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     3661 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     3721 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     3781 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     3841 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     3901 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     3961 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     4021 ttttcctttg aaaaacacga taataccatg gccaccgagt acaagcccac ggtgcgcctc
     4081 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     4141 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     4201 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     4261 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     4321 atcggctcgc gcatggccaa gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4381 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     4441 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     4501 gccgagcgcg ctggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     4561 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     4621 tggtgcatga cccgcaagcc cggtgcctga gcggccgcaa tggatcctgt ttgaatgagg
     4681 cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg ggattacttc
     4741 ttcaggttaa cccaacagaa ggctaaagaa ggtatattgc tgttgacagt gagcgnnnnn
     4801 nnnnnnnnnn nnnnnnntag tgaagccaca gatgtannnn nnnnnnnnnn nnnnnnnntg
     4861 cctactgcct cggacttcaa ggggctactt taggagcaat tatcttgttt actaaaactg
     4921 aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa
     4981 atcactttac gcgtggcgcg cctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     5041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5401 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5581 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     5641 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     5701 gaggaggagg tgggtttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     5761 ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag ggctaattca
     5821 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     5881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     5941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     6061 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg
     6121 tttaaactgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     6421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     6481 tatatccatt acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6961 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     7021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7081 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8281 ccccgaaaag tgccacctga cgtc
//
