LOCUS       40924_48398       14426 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Zika virus vector pZIKV-ICD, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Tsetsarkin KA, Kenney H, Chen R, Liu G, Manukyan H, Whitehead SS, 
            Laassri M, Chumakov K, Pletnev AG.
  TITLE     A Full-Length Infectious cDNA Clone of Zika Virus from the 2015 
            Epidemic in Brazil as a Genetic Platform for Studies of Virus-Host 
            Interactions and Vaccine Development
  JOURNAL   MBio 7 (4), e01114-16 (2016)
  PUBMED    27555311
REFERENCE   2  (bases 1 to 14426)
  AUTHORS   Tsetsarkin KA, Pletnev AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2016) Neurotropic Flaviviruses Section; Laboratory
            of Infectious Diseases, National Institute of Allergy and Infectious
            Diseases, 3 North Dr Rm 3W10A, Bethesda, MD 20892-3203, USA
REFERENCE   3  (bases 1 to 14426)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14426)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio"; 
            date: "2016"; volume: "7"; issue: "4"; pages: "e01114-16"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2016) Neurotropic Flaviviruses Section; Laboratory of 
            Infectious Diseases, National Institute of Allergy and Infectious 
            Diseases, 3 North Dr Rm 3W10A, Bethesda, MD 20892-3203, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          9089..11073
                     /isolate="Paraiba_01/2015"
                     /mol_type="other DNA"
                     /db_xref="taxon:64320"
                     /organism="Zika virus"
     CDS             1874..1885
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     intron          2712..2844
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(8609..8620)
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
     intron          8956..9088
                     /note="derived from commercial plasmid pCI-neo"
     polyA_signal    11283..11364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    11399..11454
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(12181..12725)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(12893..13750)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(13751..13855)
                     /label=AmpR promoter
     enhancer        13904..14207
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        14208..14411
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac
       61 agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa
      121 aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga
      181 gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca
      241 ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc
      301 tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca
      361 agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag
      421 gcgcagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg
      481 tcactagacg tgggagtgca tactatatgt acttggacag aaacgatgct ggggaggcca
      541 tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac
      601 acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag
      661 atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc
      721 acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta
      781 ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcatttga
      841 ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg
      901 cttggctttt gggaagctca acaagccaaa aagtcatata cttggtcatg atactgctga
      961 ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta
     1021 tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg
     1081 cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg
     1141 aggtaagatc ctactgctat gaggcatcaa tatcagacat ggcttcggac agccgctgcc
     1201 caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa
     1261 cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga
     1321 catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc
     1381 tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg
     1441 acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa
     1501 gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag
     1561 gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggttcaca
     1621 aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac
     1681 actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg
     1741 tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg
     1801 ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa
     1861 tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca
     1921 ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga
     1981 cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag
     2041 ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga
     2101 tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga
     2161 agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg
     2221 tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg
     2281 gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat
     2341 cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt
     2401 tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt
     2461 tgatcttctt atccacagcc gtctctgctg atgtggggtg ctcggtggac ttctcaaaga
     2521 aggagacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca
     2581 ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg
     2641 aagatggtat ctgcgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag
     2701 tagaagggga ggtaagtatc aaggttacaa ggcaggttta aggagaccaa tagaaactgg
     2761 gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt cttactgaca
     2821 tccactttgc ctttctctcc acagctcaac gcaatcctgg aagagaatgg agttcaactg
     2881 acggtcgttg tgggatctgt aaaaaacccc atgtggagag gtccacagag attgcccgtg
     2941 cctgtgaacg agctgcccca cggctggaag gcttggggga aatcgtactt cgtcagagca
     3001 gcaaagacaa ataacagctt tgtcgtggat ggtgacacac tgaaggaatg cccactcaaa
     3061 catagagcat ggaacagctt tcttgtggag gatcatgggt tcggggtatt tcacactagt
     3121 gtctggctca aggttagaga agattattca ttagagtgtg atccagccgt tattggaaca
     3181 gctgttaagg gaaaggaggc tgtacacagt gatctaggct actggattga gagtgagaag
     3241 aatgacacat ggaggctgaa gagggcccac ctgatcgaga tgaaaacatg tgaatggcca
     3301 aagtcccaca cattgtggac agatggaata gaagagagtg atctgatcat acccaagtct
     3361 ttagctgggc cactcagcca tcacaatacc agagagggct acaggaccca aatgaaaggg
     3421 ccatggcaca gtgaagagct tgaaattcgg tttgaggaat gcccaggcac taaggtccac
     3481 gtggaggaaa catgtggaac aagaggacca tctctgagat caaccactgc aagcggaagg
     3541 gtgatcgagg aatggtgctg cagggagtgc acaatgcccc cactgtcgtt ccgggctaaa
     3601 gatggctgtt ggtatggaat ggagataagg cccaggaaag aaccagaaag caacttagta
     3661 aggtcagtgg tgactgcagg atcaactgat cacatggatc acttctccct tggagtgctt
     3721 gtgattctgc tcatggtgca ggaagggctg aagaagagaa tgaccacaaa gatcatcata
     3781 agcacatcaa tggcagtgct ggtagctatg atcctgggag gattttcaat gagtgacctg
     3841 gctaagcttg caattttgat gggtgccacc ttcgcggaaa tgaacactgg aggagatgta
     3901 gctcatctgg cgctgatagc ggcattcaaa gtcagaccag cgttgctggt atctttcatc
     3961 ttcagagcta attggacacc ccgtgaaagc atgctgctgg ccttggcctc gtgtcttttg
     4021 caaactgcga tctccgcctt ggaaggcgac ctgatggttc tcatcaatgg ttttgctttg
     4081 gcctggttgg caatacgagc gatggttgtt ccacgcactg ataacatcac cttggcaatc
     4141 ctggctgctc tgacaccact ggcccggggc acactgcttg tggcgtggag agcaggcctt
     4201 gctacttgcg gggggtttat gctcctctct ctgaagggaa aaggcagtgt gaagaagaac
     4261 ttaccatttg tcatggccct gggactaacc gctgtgaggc tggtcgaccc catcaacgtg
     4321 gtgggactgc tgttgctcac aaggagtggg aagcggagct ggccccctag cgaagtactc
     4381 acagctgttg gcctgatatg cgcattggct ggagggttcg ccaaggcaga tatagagatg
     4441 gctgggccca tggccgcggt cggtctgcta attgtcagtt acgtggtctc aggaaagagt
     4501 gtggacatgt acattgaaag agcaggtgac atcacatggg aaaaagatgc ggaagtcact
     4561 ggaaacagtc cccggctcga tgtggcgcta gatgagagtg gtgatttctc cctggtggag
     4621 gatgacggtc cccccatgag agagatcata ctcaaggtgg tcctgatgac catctgtggc
     4681 atgaacccaa tagccatacc ctttgcagct ggagcgtggt acgtatacgt gaagactgga
     4741 aaaaggagtg gtgctctatg ggatgtgcct gctcccaagg aagtaaaaaa gggggagacc
     4801 acagatggag tgtacagagt aatgactcgt agactgctag gttcaacaca agttggagtg
     4861 ggagttatgc aagagggggt ctttcacact atgtggcacg tcacaaaagg atccgcgctg
     4921 agaagcggtg aagggagact tgatccatac tggggagatg tcaagcagga tctggtgtca
     4981 tactgtggtc catggaagct agatgccgcc tgggacgggc acagcgaggt gcagctcttg
     5041 gccgtgcccc ccggagagag agcgaggaac atccagactc tgcccggaat atttaagaca
     5101 aaggatgggg acattggagc ggttgcgctg gattacccag caggaacttc aggatctcca
     5161 atcctagaca agtgtgggag agtgatagga ctttatggca atggggtcgt gatcaaaaat
     5221 gggagttatg ttagtgccat cacccaaggg aggagggagg aagagactcc tgttgagtgc
     5281 ttcgagcctt cgatgctgaa gaagaagcag ctaactgtct tagacttgca tcctggagct
     5341 gggaaaacca ggagagttct tcctgaaata gtccgtgaag ccataaaaac aagactccgt
     5401 actgtgatct tagctccaac cagggttgtc gctgctgaaa tggaggaagc ccttagaggg
     5461 cttccagtgc gttatatgac aacagcagtc aatgtcaccc actctggaac agaaatcgtc
     5521 gacttaatgt gccatgccac cttcacttca cgtctactac agccaatcag ggtccccaac
     5581 tataatctgt atattatgga tgaggcccac ttcacagatc cctcaagtat agcagcaaga
     5641 ggatacattt caacaagggt tgagatgggc gaggcggctg ccatcttcat gaccgccacg
     5701 ccaccaggaa cccgtgacgc atttccggac tccaactcac caattatgga caccgaagtg
     5761 gaagtcccag agagagcctg gagctcaggc tttgattggg tgacggatca ttctggaaaa
     5821 acagtttggt ttgttccaag cgtgaggaac ggcaatgaga tcgcagcttg tctgacgaag
     5881 gctggaaaac gggtcataca gctcagcaga aagacttttg agacagagtt ccagaaaaca
     5941 aaacatcaag agtgggactt tgtcgtgaca actgacattt cagagatggg cgccaacttt
     6001 aaagctgacc gtgtcataga ttccaggaga tgcctaaagc cggtcatact tgatggcgag
     6061 agagtcattc tggctggacc catgcctgtc acacatgcca gcgctgccca gaggaggggg
     6121 cgcataggca ggaatcccaa caaacctgga gatgagtatc tgtatggagg tgggtgcgca
     6181 gagactgacg aagaccatgc acactggctt gaagcaagaa tgctccttga caatatttac
     6241 ctccaagatg gcctcatagc ctcgctctat cgacctgagg ccgacaaagt agcagccatt
     6301 gagggagagt tcaagcttag gacggagcaa aggaagacct ttgtggaact catgaaaaga
     6361 ggagatcttc ctgtttggct ggcctatcag gttgcatctg ccggaataac ctacacagat
     6421 agaagatggt gctttgatgg cacgaccaac aataccataa tggaagacag tgtgccggca
     6481 gaggtgtgga ccagacacgg agagaaaaga gtgctcaaac cgaggtggat ggacgccaga
     6541 gtttgttcag atcatgcggc cctgaagtca ttcaaggagt ttgccgctgg gaaaagagga
     6601 gcggcttttg gagtgatgga agccctggga acactgccag gacacatgac agagagattc
     6661 caggaagcca ttgacaacct cgctgtgctc atgcgggcag agactggaag caggccttac
     6721 aaagccgcgg cggcccaatt gccggagacc ctagagacca ttatgctttt ggggttgctg
     6781 ggaacagtct cgctgggaat ctttttcgtc ttgatgagga acaagggcat agggaagatg
     6841 ggctttggaa tggtgactct tggggccagc gcatggctca tgtggctctc ggaaattgag
     6901 ccagccagaa ttgcatgtgt cctcattgtt gtgttcctat tgctggtggt gctcatacct
     6961 gagccagaaa agcaaagatc tccccaggac aaccaaatgg caatcatcat catggtagca
     7021 gtaggtcttc tgggcttgat taccgccaat gaactcggat ggttggagag aacaaagagt
     7081 gacctaagcc acctaatggg aaggagagag gagggagcaa ccataggatt ctcaatggac
     7141 attgacctgc ggccagcctc agcttgggcc atctatgctg ccttgacaac tttcattacc
     7201 ccagccgtcc aacatgcagt gaccacttca tacaacaact actccttaat ggcgatggcc
     7261 acgcaagctg gagtgttgtt tggtatgggc aaagggatgc cattctacgc atgggacttt
     7321 ggagtcccgc tgctaatgat aggttgctac tcacaattaa cacccctgac cctaatagtg
     7381 gccatcattt tgctcgtggc gcactacatg tacttgatcc cagggctgca ggcagcagct
     7441 gcgcgtgctg cccagaagag aacggcagct ggcatcatga agaaccctgt tgtggatgga
     7501 atagtggtga ctgacattga cacaatgaca attgaccccc aagtggagaa aaagatggga
     7561 caggtgctac tcatagcagt agccgtctcc agcgccatac tgtcgcggac cgcctggggg
     7621 tggggagagg ctggggccct gatcacagcc gcaacttcca ctttgtggga aggctctccg
     7681 aacaagtact ggaactcctc tacagccact tcactgtgta acatttttag gggaagttac
     7741 ttggctggag cttctctaat ctacacagta acaagaaacg ctggcttggt caagagacgt
     7801 gggggtggaa caggagagac cctgggagag aaatggaagg cccgcttgaa ccagatgtcg
     7861 gccctggagt tctactccta caaaaagtca ggcatcaccg aggtgtgcag agaagaggcc
     7921 cgccgcgccc tcaaggacgg tgtggcaacg ggaggccatg ctgtgtcccg aggaagtgca
     7981 aagctgagat ggttggtgga gcggggatac ctgcagccct atggaaaggt cattgatctt
     8041 ggatgtggca gagggggctg gagttactac gccgccacca tccgcaaagt tcaagaagtg
     8101 aaaggataca caaaaggagg ccctggtcat gaagaacccg tgttggtgca aagctatggg
     8161 tggaacatag tccgtcttaa gagtggggtg gacgtctttc atatggcggc tgagccgtgt
     8221 gacacgttgc tgtgtgacat aggtgagtca tcatctagtc ctgaagtgga agaagcacgg
     8281 acgctcagag tcctctccat ggtgggggat tggcttgaaa aaagaccagg agccttttgt
     8341 ataaaagtgt tgtgcccata caccagcact atgatggaaa ccctggagcg actgcagcgt
     8401 aggtatgggg gaggactggt cagagtgcca ctctcccgca actctacaca tgagatgtac
     8461 tgggtctctg gagcgaaaag caacaccata aaaagtgtgt ccaccacgag ccagctcctc
     8521 ttggggcgca tggacgggcc aaggaggcca gtgaaatatg aggaggatgt gaatctcggc
     8581 tctggcacgc gggctgtggt aagctgcgct gaagctccca acatgaagat cattggtaac
     8641 cgcattgaaa ggatccgcag tgagcacgcg gaaacgtggt tctttgacga gaaccaccca
     8701 tataggacat gggcttacca tggaagctat gaggccccca cacaagggtc agcgtcctct
     8761 ctaataaacg gggttgtcag gctcctgtca aaaccctggg atgtggtgac tggagtcaca
     8821 ggaatagcca tgaccgacac cacaccgtat ggtcagcaaa gagttttcaa ggaaaaagtg
     8881 gacactaggg tgccagaccc ccaagaaggc actcgtcagg ttatgagcat ggtctcttcc
     8941 tggttgtgga aagaggtaag tatcaaggtt acaaggcagg tttaaggaga ccaatagaaa
     9001 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
     9061 gacatccact ttgcctttct ctccacagct aggcaaacac aaacggccac gagtctgtac
     9121 caaagaagag tttatcaaca aggttcgtag caatgcagca ttaggggcaa tatttgaaga
     9181 ggaaaaagag tggaagactg cagtggaagc tgtgaacgat ccaaggttct gggctctagt
     9241 ggacaaggaa agagagcacc acctgagagg agagtgccag agttgtgtgt acaacatgat
     9301 gggaaaaaga gaaaagaaac aaggggaatt tggaaaggcc aagggcagcc gcgccatctg
     9361 gtatatgtgg ctaggggcta gatttctaga gttcgaagcc cttggattct tgaacgagga
     9421 tcactggatg gggagagaga actcaggagg tggtgttgaa gggctgggat tacaaagact
     9481 cggatatgtc ctagaagaga tgagtcgcat accaggagga aggatgtatg cagatgacac
     9541 tgctggctgg gacacccgca tcagcaggtt tgatctggag aatgaagctc taatcaccaa
     9601 ccaaatggag aaagggcaca gggccttggc attggccata atcaagtaca cataccaaaa
     9661 caaagtggta aaggtcctta gaccagctga aaaagggaaa acagttatgg acattatttc
     9721 gagacaagac caaaggggga gcggacaagt tgtcacttac gctcttaaca catttaccaa
     9781 cctagtggtg caactcattc ggaatatgga ggctgaggaa gttctggaga tgcaagactt
     9841 gtggctgctg cggaggtcag agaaagtgac caactggttg cagagcaacg gatgggatag
     9901 gctcaaacga atggcagtca gtggagatga ttgcgttgtg aagccaattg atgataggtt
     9961 tgcacatgcc ctcaggttct tgaatgatat gggaaaagtt aggaaggaca cacaagagtg
    10021 gaaaccctca actggatggg acaactggga agaagttccg ttttgctccc accacttcaa
    10081 caagctccat ctcaaggacg ggaggtccat tgtggttccc tgccgccacc aagatgaact
    10141 gattggccgg gcgcgcgtct ctccaggggc gggatggagc atccgggaga ctgcttgcct
    10201 agcaaaatca tatgcgcaaa tgtggcagct cctttatttc cacagaaggg acctccgact
    10261 gatggccaat gccatttgtt catctgtgcc agttgactgg gttccaactg ggagaactac
    10321 ctggtcaatc catggaaagg gagaatggat gaccactgaa gacatgcttg tggtgtggaa
    10381 cagagtgtgg attgaggaga acgaccacat ggaagacaag accccagtta cgaaatggac
    10441 agacattccc tatttgggaa aaagggaaga cttgtggtgt ggatctctca tagggcacag
    10501 accgcgcacc acctgggctg agaacattaa aaacacagtc aacatggtgc gcaggatcat
    10561 aggtgatgaa gaaaagtaca tggactacct atccacccaa gttcgctact tgggtgaaga
    10621 agggtctaca cctggagtgc tgtaagcacc aatcttaatg ttgtcaggcc tgctagtcag
    10681 ccacagcttg gggaaagctg tgcagcctgt gaccccccca ggagaagctg ggaaaccaag
    10741 cctatagtca ggccgagaac gccatggcac ggaagaagcc atgctgcctg tgagcccctc
    10801 agaggacact gagtcaaaaa accccacgcg cttggaggcg caggatggga aaagaaggtg
    10861 gcgaccttcc ccacccttca atctggggcc tgaactggag atcagctgtg gatctccaga
    10921 agagggacta gtggttagag gagacccccc ggaaaacgca aaacagcata ttgacgctgg
    10981 gaaagaccag agactccatg agtttccacc acgctggccg ccaggcacag atcgccgaat
    11041 agcggcggcc ggtgtgggga aatccatggg tctgggtcgg catggcatct ccacctcctc
    11101 gcggtccgac ctgggcatcc gaaggaggac gcacgtccac tcggatggct aagggagagc
    11161 cacgagctcc tcgacagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
    11221 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
    11281 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    11341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaagataa
    11401 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
    11461 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
    11521 gcaacatatg cccatatgta agatatcagg cttcgagcaa gacgtttccc gttgaatatg
    11581 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
    11641 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg
    11701 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
    11761 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
    11821 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
    11881 gtcagcaaca ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac
    11941 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
    12001 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
    12061 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
    12121 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
    12181 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
    12241 gcgaaacccg acaggactat aaagatacca ggcgtttccc ctggcggctc cctcgtgcgc
    12301 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
    12361 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
    12421 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    12481 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
    12541 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
    12601 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcgaaaaac
    12661 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
    12721 ctcaagaaga tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag
    12781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    12841 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    12901 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    12961 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    13021 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    13081 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    13141 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    13201 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    13261 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    13321 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    13381 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    13441 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    13501 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    13561 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    13621 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    13681 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    13741 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    13801 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    13861 ttccccgaaa agtgccacct gacgtgtcga cgcggccgca tgtcgttaca taacttacgg
    13921 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
    13981 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    14041 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    14101 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
    14161 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
    14221 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
    14281 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
    14341 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
    14401 taagcagagc tcgtttagtg aaccgt
//
