LOCUS       40924_48383        3991 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a promoter that can be activated by 
            drug-induced heterodimerization of ZFHD1 and the p65 activation 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    88..297
                     /label=12  ZFHD1 binding sites
                     /bound_moiety="ZFHD1"
                     /note="ZFHD1 binding sites"
                     /note="12 binding sites for the composite human DNA-binding
                     domain ZFHD1 (Pomerantz et al., 1995)"
     promoter        320..433
                     /label=IL-2 promoter
                     /note="minimal promoter from the human interleukin-2 gene"
     misc_feature    462..505
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    619..740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(747..1202)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1229..1333
                     /label=AmpR promoter
     promoter        1335..1692
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1727..2518
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2753..2800
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3129..3717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3779..3827
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3841..3932
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc ggcttacgcg tgctagctaa tgatgggcgc tcgagtaatg atgggcggtc
      121 gactaatgat gggcgctcga gtaatgatgg gcgtctagct aatgatgggc gctcgagtaa
      181 tgatgggcgg tcgactaatg atgggcgctc gagtaatgat gggcgtctag ctaatgatgg
      241 gcgctcgagt aatgatgggc ggtcgactaa tgatgggcgc tcgagtaatg atgggcgtct
      301 agaacgcgaa ttaattcaac attttgacac ccccataata tttttccaga attaacagta
      361 taaattgcat ctcttgttca agagttccct atcactctct ttaatcacta ctcacagtaa
      421 cctcaactcc tgccacaagc ttgccctgca gcgggaattc caccggtggg ccccccgggg
      481 gatccactag ttgatcagcg gccgcgactc tagatcataa tcagccatac cacatttgta
      541 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
      601 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      661 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      721 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc
      781 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
      841 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
      901 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
      961 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     1021 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     1081 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     1141 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     1201 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1261 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1321 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     1381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     1441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     1501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     1561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     1621 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     1681 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     1741 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     1801 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     1861 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     1921 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     1981 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     2041 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     2101 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     2161 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     2221 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     2281 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     2341 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     2401 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     2461 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     2521 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     2581 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     2641 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     2701 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     2761 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     2821 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     2881 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     2941 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     3001 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3061 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3121 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3181 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3241 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3301 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3361 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3421 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3481 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3541 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3601 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3661 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3721 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtaa
     3781 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     3841 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     3901 cagtgcaagt gcaggtgcca gaacatttct ctacatgttc tttcctgcgt tatcccctga
     3961 ttctgtggat aaccgtatta ccgccatgca t
//
