LOCUS       pZE21-MCS               2265 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039151
VERSION     V039151
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        6..79
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
                     /label="PLtetO-1 promoter"
     RBS             89..100
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
                     /label="RBS"
     terminator      207..293
                     /gene="Escherichia coli rrnB"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /label="rrnB T1 terminator"
     rep_origin      complement(458..1046)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     terminator      complement(1134..1228)
                     /gene=""
                     /note="transcription terminator from phage lambda"
                     /label="lambda t0 terminator"
     CDS             complement(1259..2053)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
ORIGIN
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccgcac cggcatatgg
      121 ctagcgcggc cgcttaatta agtcgacaag cttgatatcg aattcctgca gcccggggga
      181 tcccatggta cgcgtgctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      241 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
      301 cctagaccta gggcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
      901 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      961 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1021 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1081 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gactagtgct tggattctca
     1141 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1201 gtcattactg gatctatcaa caggagtcca agcgagctct cgaaccccag agtcccgctc
     1261 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     1321 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     1381 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     1441 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     1501 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     1561 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     1621 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     1681 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     1741 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     1801 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     1861 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     1921 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     1981 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     2041 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc
     2101 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     2161 taccagaggg cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat
     2221 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacc
//