LOCUS       40924_48338        9863 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZD_1-2-TRE-TALER14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2015) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 9863)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JAN-2015) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..861)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(862..966)
                     /label=AmpR promoter
     rep_origin      complement(992..1447)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     1589..1605
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1615..1633
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1648..2451
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    2530..2555
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    3220..3240
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    3285..3303
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3320..3338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3356..3374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3392..3410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3427..3445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3463..3481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3493..3531
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    3571..3595
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3621..6770
                     /label=TALER14
                     /note="TALER14"
     CDS             6780..6800
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     protein_bind    complement(6916..6940)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7095..7150
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7511..7527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7535..7551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7559..7589)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7604..7625
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    7798..8634
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(8664..8682)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8703..8719)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8727..8743)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8751..8781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8796..8817)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(9105..9693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     1021 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1081 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1141 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     1201 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     1261 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     1321 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     1381 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     1441 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     1501 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     1561 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     1621 gactcactat agggcgaatt gggtacctgc tttctctgac cagcattctc tcccctgggc
     1681 ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc tctacggctg gcccagatcc
     1741 ttccctgccg cctccttcag gttccgtctt cctccactcc ctcttcccct tgctctctgc
     1801 tgtgttgctg cccaaggatg ctctttccgg agcacttcct tctcggcgct gcaccacgtg
     1861 atgtcctctg agcggatcct ccccgtgtct gggtcctctc cgggcatctc tcctccctca
     1921 cccaacccca tgccgtcttc actcgctggg ttcccttttc cttctccttc tggggcctgt
     1981 gccatctctc gtttcttagg atggccttct ccgacggatg tctcccttgc gtcccgcctc
     2041 cccttcttgt aggcctgcat catcaccgtt tttctggaca accccaaagt accccgtctc
     2101 cctggcttta gccacctctc catcctcttg ctttctttgc ctggacaccc cgttctcctg
     2161 tggattcggg tcacctctca ctcctttcat ttgggcagct cccctacccc ccttacctct
     2221 ctagtctgtg ctagctcttc cagccccctg tcatggcatc ttccaggggt ccgagagctc
     2281 agctagtctt cttcctccaa cccgggcccc tatgtccact tcaggacagc atgtttgctg
     2341 cctccaggga tcctgtgtcc ccgagctggg accaccttat attcccaggg ccggttaatg
     2401 tggctctggt tctgggtact tttatctgtc ccctccaccc cacagtgggg caagcttatt
     2461 accctgttat ccctaaccgg tttaccgagc tcttattggt tttcaaactt cattgactgt
     2521 gccaagcttc tgacctcttc tcttcctccc acagggcctc gagacaattg attaacatcg
     2581 atacggtacc gagttggcgc gcctgggagc tcacggggac agcccccccc caaagccccc
     2641 agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc ggggctccgc
     2701 tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca gcccgggcac
     2761 ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt tgagcctgca
     2821 gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta gaatgacagg
     2881 cgcgcctggc catacatcga tacggtaccg agttggcgcg cctgggagct cacggggaca
     2941 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     3001 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     3061 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     3121 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag
     3181 agagatttag aatgacaggc gcgcctggtg gcgaacgatc aactttgtat agaaaagttg
     3241 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
     3301 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
     3361 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     3421 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     3481 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     3541 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc aggctgatta
     3601 ccggagaatt ccaattgacc atgaccggtt tgtcgaccat tcgtccgcgc aggccaagtc
     3661 ctgcccgcga gcttctgccc ggaccccaac cggatagggt tcagccgact gcagatcgtg
     3721 gggtgtctgc gcctgctggc agccctctgg atggcttgcc cgctcggcgg acggtgtccc
     3781 ggacccggct gccatctccc cctgcgccct cacctgcgtt ctcggcgggc agcttcagcg
     3841 atctgctccg tccgttcgat ccgtcgcttc ttgatacatc gcttcttgat tcgatgcctg
     3901 ccgtcggcac gccgcataca gcggctgccc cagcagagtg ggatgaggcg caatcggctc
     3961 tgcgtgcagc cgatgacccg ccacccaccg tgcgtgtcgc tgtcactgcc gcgcggccgc
     4021 cgcgcgccaa gccggccccg cgacggcgtg ctgcgcaacc ctccgacgct tcgccggccg
     4081 cgcaggtgga tctacgcacg ctcggctaca gtcagcagca gcaagagaag atcaaaccga
     4141 aggtgcgttc gacagtggcg cagcaccacg aggcactggt gggccatggg tttacacacg
     4201 cgcacatcgt tgcgctcagc caacacccgg cagcgttagg gaccgtcgct gtcacgtatc
     4261 agcacataat cacggcgttg ccagaggcga cacacgaaga catcgttggc gtcggcaaac
     4321 agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga tgcgggggag ttgagaggtc
     4381 cgccgttaca gttggacaca ggccaacttg tgaagattgc aaaacgtggc ggcgtgaccg
     4441 caatggaggc agtgcatgca tcgcgcaatg cactgacggg tgcccccctg aacctgaccc
     4501 cggaccaagt ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc
     4561 agcggctgtt gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta
     4621 tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     4681 tgtgccagga ccatggcctg actccggacc aagtggtggc tatcgccagc cacgatggcg
     4741 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     4801 tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa
     4861 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg
     4921 tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     4981 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg
     5041 gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
     5101 atggcctgac tccggaccaa gtggtggcta tcgccagcca cgatggcggc aagcaagcgc
     5161 tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg accccggacc
     5221 aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc
     5281 tgttgccggt gctgtgccag gaccatggcc tgactccgga ccaagtggtg gctatcgcca
     5341 gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc
     5401 aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat ggcggcaagc
     5461 aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc
     5521 cggaccaagt ggtggctatc gccagcaaca atggcggcaa gcaagcgctc gaaacggtgc
     5581 agcggctgtt gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta
     5641 tcgccagcca cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc
     5701 tgtgccagga ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg
     5761 gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc
     5821 tgaccccgga ccaagtggtg gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa
     5881 cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg
     5941 tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag cggctgttgc
     6001 cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc gccagcaacg
     6061 gtggcggcaa gcaagcgctc gaaagcattg tggcccagct gagccggcct gatccggcgt
     6121 tggccgcgtt gaccaacgac cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca
     6181 tggatgcagt gaaaaaggga ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc
     6241 gtattggcga acgcacgtcc catcgcgttg ccgactacgc gcaagtggtt cgcgtgctgg
     6301 agtttttcca gtgccactcc cacccagcgt acgcatttga tgaggccatg acgcagttcg
     6361 ggatgagcag gaacgggttg gtacagctct ttcgcagagt gggcgtcacc gaactcgaag
     6421 cccgcggtgg aacgctcccc ccagcctcgc agcgttggga ccgtatcctc caggcatcag
     6481 ggatgaaaag ggccaaaccg tcccctactt cagctcaaac accggatcag gcgtctttgc
     6541 atgcattcgc cgattcgctg gagcgtgacc ttgatgcgcc cagcccaatg cacgagggag
     6601 atcagacgcg ggcaagcagc cgtaaacggt cccgatcgga tcgtgctgtc accggcccct
     6661 ccgcacagca ggctgtcgag gtgcgcgttc ccgaacagcg cgatgcgctg catttgcccc
     6721 tcagctggag ggtaaaacgc ccgcgtacca ggatctgggg cggcctcccg gatcccagcc
     6781 ccaagaagaa gagaaaggtg gaggccagcg gtggcggctc aaagcttggt ggcggctcaa
     6841 ctagttaagg gcccggcgcg cctaaggtac ccccgggtaa ctgatcaaga tccaccggat
     6901 ctagataact gatctaccca gctttcttgt acaaagtggt acgcgtgaat tcactcctca
     6961 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     7021 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     7081 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     7141 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     7201 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     7261 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     7321 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     7381 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     7441 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     7501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     7681 tcgacagtac taagcttggt gcgtttttat gcttgtagta ttgtataatg tttttaagat
     7741 ccttaattaa tagggataac agggtaatct cgaggtttaa acgtcgacgc ggccgctact
     7801 agggacagga ttggtgacag aaaagcccca tccttaggcc tcctccttcc tagtctcctg
     7861 atattgggtc taacccccac ctcctgttag gcagattcct tatctggtga cacaccccca
     7921 tttcctggag ccatctctct ccttgccaga acctctaagg tttgcttacg atggagccag
     7981 agaggatcct gggagggaga gcttggcagg gggtgggagg gaaggggggg atgcgtgacc
     8041 tgcccggttc tcagtggcca ccctgcgcta ccctctccca gaacctgagc tgctctgacg
     8101 cggctgtctg gtgcgtttca ctgatcctgg tgctgcagct tccttacact tcccaagagg
     8161 agaagcagtt tggaaaaaca aaatcagaat aagttggtcc tgagttctaa ctttggctct
     8221 tcacctttct agtccccaat ttatattgtt cctccgtgcg tcagttttac ctgtgagata
     8281 aggccagtag ccagccccgt cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga
     8341 aaactccctt tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact
     8401 ccctttctct ttctccatcc ttctttcctt aaagagtccc cagtgctatc tgggacatat
     8461 tcctccgccc agagcagggt cccgcttccc taaggccctg ctctgggctt ctgggtttga
     8521 gtccttggca agcccaggag aggcgctcag gcttccctgt cccccttcct cgtccaccat
     8581 ctcatgcccc tggctctcct gccccttccc tacaggggtt cctggctctg ctctccaccg
     8641 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     8701 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8761 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8821 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9841 tatgagtaaa cttggtctga cag
//
