LOCUS       40924_48333        7528 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pZC320, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Shi J, Biek DP.
  TITLE     A versatile low-copy-number cloning vector derived from plasmid F
  JOURNAL   Gene 164 (1), 55-58 (1995)
  PUBMED    7590321
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   Mori H, Kondo A, Ohshima A, Ogura T, Hiraga S.
  TITLE     Structure and function of the F plasmid genes essential for 
            partitioning
  JOURNAL   J. Mol. Biol. 192 (1), 1-15 (1986)
  PUBMED    3029390
REFERENCE   3  (bases 1 to 7528)
  AUTHORS   Prentki P, Binda A, Epstein A.
  TITLE     Plasmid vectors for selecting IS1-promoted deletions in cloned DNA: 
            sequence analysis of the omega interposon
  JOURNAL   Gene 103 (1), 17-23 (1991)
  PUBMED    1652541
REFERENCE   4  (bases 1 to 7528)
  AUTHORS   Biek DP.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   5  (bases 1 to 7528)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 7528)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1995"; volume: "164"; issue: "1"; pages: "55-58"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "1986"; volume: "192"; issue: "1"; pages: "1-15"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Gene"; 
            date: "1991"; volume: "103"; issue: "1"; pages: "17-23"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1995) Donald P. Biek, Department of Microbiology "
COMMENT     SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      74..293
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             384..1136
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    1142..1392
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             1718..2890
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             2893..3861
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    3937..4410
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     regulatory      4785..4922
                     /standard_name="omega terminator"
                     /label=omega terminator
                     /note="contains the T4 gene 32 terminator and a portion of
                     the polylinker from the omega interposon"
                     /regulatory_class="terminator"
     CDS             complement(5252..6109)
                     /label=AmpR
                     /note="beta-lactamase"
     CDS             complement(6122..6181)
                     /codon_start=1
                     /gene="aadA'"
                     /product="aminoglycoside 3'-acetyltransferase"
                     /number=1
                     /label=aadA'
                     /note="encodes the first 18 amino acids of AadA and one
                     from the vector before stopping 12 bp from the beginning of
                     bla coding region"
                     /protein_id="AAC53639.1"
                     /translation="MREAVIAEVSTQLSEVVGD"
     gene            complement(6122..6181)
                     /gene="aadA'"
                     /label=aadA'
     primer_bind     6918..6934
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7027..7045)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7067..7083)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7091..7107)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7115..7145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7160..7181)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      complement(7399..7528)
                     /standard_name="omega terminator"
                     /label=omega terminator
                     /note="contains the T4 gene 32 terminator and portion of
                     the polylinker from the omega interposon"
                     /regulatory_class="terminator"
ORIGIN
        1 tcgagtgagc gaggaagcac cagggaacag cacttatata ttctgcttac acacgatgcc
       61 tgaaaaaact tcccttgggg ttatccactt atccacgggg atatttttat aattattttt
      121 tttatagttt ttagatcttc ttttttagag cgccttgtag gcctttatcc atgctggttc
      181 tagagaaggt gttgtgacaa attgcccttt cagtgtgaca aatcaccctc aaatgacagt
      241 cctgtctgtg acaaattgcc cttaaccctg tgacaaattg ccctcagaag aagctgtttt
      301 ttcacaaagt tatccctgct tattgactct tttttattta gtgtgacaat ctaaaaactt
      361 gtcacacttc acatggatct gtcatggcgg aaacagcggt tatcaatcac aagaaacgta
      421 aaaatagccc gcgaatcgtc cagtcaaacg acctcactga ggcggcatat agtctctccc
      481 gggatcaaaa acgtatgctg tatctgttcg ttgaccagat cagaaaatct gatggcaccc
      541 tacaggaaca tgacggtatc tgcgagatcc atgttgctaa atatgctgaa atattcggat
      601 tgacctctgc ggaagccagt aaggatatac ggcaggcatt gaagagtttc gcggggaagg
      661 aagtggtttt ttatcgccct gaagaggatg ccggcgatga aaaaggctat gaatcttttc
      721 cttggtttat caaacgtgcg cacagtccat ccagagggct ttacagtgta catatcaacc
      781 catatctcat tcccttcttt atcgggttac agaaccggtt tacgcagttt cggcttagtg
      841 aaacaaaaga aatcaccaat ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc
      901 gtaagccgga tggctcaggc atcgtctctc tgaaaatcga ctggatcata gagcgttacc
      961 agctgcctca aagttaccag cgtatgcctg acttccgccg ccgcttcctg caggtctgtg
     1021 ttaatgagat caacagcaga actccaatgc gcctctcata cattgagaaa aagaaaggcc
     1081 gccagacgac tcatatcgta ttttccttcc gcgatatcac ttccatgacg acaggatagt
     1141 ctgagggtta tctgtcacag atttgagggt ggttcgtcac atttgttctg acctactgag
     1201 ggtaatttgt cacagttttg ctgtttcctt cagcctgcat ggattttctc atactttttg
     1261 aactgtaatt tttaaggaag ccaaatttga gggcagtttg tcacagttga tttccttctc
     1321 tttcccttcg tcatgtgacc tgatatcggg ggttagttcg tcatcattga tgagggttga
     1381 ttatcacagt ttattactct gaattggcta tccgcgtgtg tacctctacc tggagttttt
     1441 cccacggtgg atatttcttc ttgcgctgag cgtaagagct atctgacaga acagttcttc
     1501 tttgcttcct cgccagttcg ctcgctatgc tcggttacac ggctgcggcg agcgctagtg
     1561 ataataagtg actgaggtat gtgctcttct tatctccttt tgtagtgttg ctcttatttt
     1621 aaacaacttt gcggtttttt gatgactttg cgattttgtt gttgctttgc agtaaattgc
     1681 aagatttaat aaaaaaacgc aaagcaatga ttaaaggatg ttcagaatga aactcatgga
     1741 aacacttaac cagtgcataa acgctggtca tgaaatgacg aaggctatcg ccattgcaca
     1801 gtttaatgat gacagcccgg aagcgaggaa aataacccgg cgctggagaa taggtgaagc
     1861 agcggattta gttggggttt cttctcaggc tatcagagat gccgagaaag cagggcgact
     1921 accgcacccg gatatggaaa ttcgaggacg ggttgagcaa cgtgttggtt atacaattga
     1981 acaaattaat catatgcgtg atgtgtttgg tacgcgattg cgacgtgctg aagacgtatt
     2041 tccaccggtg atcggggttg ctgcccataa aggtggcgtt tacaaaacct cagtttctgt
     2101 tcatcttgct caggatctgg ctctgaaggg gctacgtgtt ttgctcgtgg aaggtaacga
     2161 cccccaggga acagcctcaa tgtatcacgg atgggtacca gatcttcata ttcatgcaga
     2221 agacactctc ctgcctttct atcttgggga aaaggacgat gtcacttatg caataaagcc
     2281 cacttgctgg ccggggcttg acattattcc ttcctgtctg gctctgcacc gtattgaaac
     2341 tgagttaatg ggcaaatttg atgaaggtaa actgcccacc gatccacacc tgatgctccg
     2401 actggccatt gaaactgttg ctcatgacta tgatgtcata gttattgaca gcgcgcctaa
     2461 cctgggtatc ggcacgatta atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc
     2521 tgctgagttg tttgactaca cctccgcact gcagtttttc gatatgcttc gtgatctgct
     2581 caagaacgtt gatcttaaag ggttcgagcc tgatgtacgt attttgctta ccaaatacag
     2641 caatagtaat ggctctcagt ccccgtggat ggaggagcaa attcgggatg cctggggaag
     2701 catggttcta aaaaatgttg tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat
     2761 gagaactgtt tttgaacagg ccattgatca acgctcttca actggtgcct ggagaaatgc
     2821 tctttctatt tgggaacctg tctgcaatga aattttcgat cgtctgatta aaccacgctg
     2881 ggagattaga taatgaagcg tgcgcctgtt attccaaaac atacgctcaa tactcaaccg
     2941 gttgaagata cttcgttatc gacaccagct gccccgatgg tggattcgtt aattgcgcgc
     3001 gtaggagtaa tggctcgcgg taatgccatt actttgcctg tatgtggtcg ggatgtgaag
     3061 tttactcttg aagtgctccg gggtgatagt gttgagaaga cctctcgggt atggtcaggt
     3121 aatgaacgtg accaggagct gcttactgag gacgcactgg atgatctcat cccttctttt
     3181 ctactgactg gtcaacagac accggcgttc ggtcgaagag tatctggtgt catagaaatt
     3241 gccgatggga gtcgccgtcg taaagctgct gcacttaccg aaagtgatta tcgtgttctg
     3301 gttggcgagc tggatgatga gcagatggct gcattatcca gattgggtaa cgattatcgc
     3361 ccaacaagtg cttatgaacg tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct
     3421 ggaaatattt ctgcgctggc tgatgcggaa aatatttcac gtaagattat tacccgctgt
     3481 atcaacaccg ccaaattgcc taaatcagtt gttgctcttt tttctcaccc cggtgaacta
     3541 tctgcccggt caggtgatgc acttcaaaaa gcctttacag ataaagagga attacttaag
     3601 cagcaggcat ctaaccttca tgagcagaaa aaagctgggg tgatatttga agctgaagaa
     3661 gttatcactc ttttaacttc tgtgcttaaa acgtcatctg catcaagaac tagtttaagc
     3721 tcacgacatc agtttgctcc tggagcgaca gtattgtata agggcgataa aatggtgctt
     3781 aacctggaca ggtctcgtgt tccaactgag tgtatagaga aaattgaggc cattcttaag
     3841 gaacttgaaa agccagcacc ctgatgcgac cacgttttag tctacgttta tctgtcttta
     3901 cttaatgtcc tttgttacag gccagaaagc ataactggcc tgaatattct ctctgggccc
     3961 actgttccac ttgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc
     4021 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
     4081 tctgggacca cggtcccact cgtatcgtcg gtctgataat cagactggga ccacggtccc
     4141 actcgtatcg tcggtctgat tattagtctg ggaccatggt cccactcgta tcgtcggtct
     4201 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctggaac
     4261 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     4321 cgtcggtctg attattagtc tgggaccacg atcccactcg tgttgtcggt ctgattatcg
     4381 gtctgggacc acggtcccac ttgtattgtc gatcagacta tcagcgtgag actacgattc
     4441 catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt aacctgtaga acggagtaac
     4501 ctcggtgtgc ggttgtatgc ctgctgtgga ttgctgctgt gtcctgctta tccacaacat
     4561 tttgcgcacg gttatgtgga caaaatacct ggttacccag gccgtgccgg cacgttaacc
     4621 gggctgcatc cgatgcaagt gtgtcgctgt cgacggcctc ctcacccggt cacgtttcgt
     4681 cgtttctcct ccacgcgctc ggcttcgggg ccgcacctgc attcgtatgc ggtcgcccgg
     4741 ttacaggtgc ggcacggcct gatggaggcc gcatgtgaga ggagaattcc cggggatcag
     4801 ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa taaaaaaggg gacctctagg
     4861 gtccccaatt aattagtaat ataatctatt aaaggtcatt caaaaggtca tccaccggat
     4921 cagcttagta aagccctcgc tagattttaa tgcggatgtt gcgattactt cgccaactat
     4981 tgcgataaca agaaaaagcc agcctttcat gatatatctc ccaatttgtg tagggcttat
     5041 tatgcacgct taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta
     5101 tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc gaagcggcgc atgagattat
     5161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     5581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     5881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6121 ttcaatcgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     6181 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     6241 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     6301 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     6361 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct
     6421 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
     6481 gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg
     6541 agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg
     6601 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc
     6661 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag
     6721 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg attaacgctt acaatttcca
     6781 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     6841 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     6901 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gccggatcca cgtgatcgat
     6961 gcatacgcgt tcgaaagctt cgcgacgtac gacgtccgcg gagctctcga gcatgctagc
     7021 ggccgcccta tagtgagtcg tattacgcgc gcttggcgta atcatggtca tagctgtttc
     7081 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7141 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7201 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcgga tcagtgaggg
     7261 tttgcaactg cgggtcaagg atctggattt cgatcacggc acgatcatcg tgcgggaggg
     7321 caagggctcc aaggatcggg ccttgatgtt acccgagagc ttggcaccca gcctgcgcga
     7381 gcaggggaat tgatccggtg gatgaccttt tgaatgacct ttaatagatt atattactaa
     7441 ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac aaaacggttt
     7501 acaagcataa agctgatccc cgggaatt
//
