LOCUS       62056_23070        5425 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast TRP LIII A-B, complete sequence.
ACCESSION   MK495796
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 5425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..5425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(43..855)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1080..1668
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1938..1968
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2022..2678
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3023..3325
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     promoter        3397..3678
                     /label=TRP1 promoter
     CDS             3679..4350
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
     regulatory      4354..4399
                     /label=TRP1 Terminator
                     /note="TRP1 Terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(4425..5407)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgcccctt acgttgtctt caaagctgaa
     1801 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     1861 actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct cgaggggccg
     1921 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     1981 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2041 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2101 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2161 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2221 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2281 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2341 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     2401 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag
     2461 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     2521 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     2581 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     2641 aattacaaca gtactgcgat gagtggcagg gcggggcgta atctagagga tccggcttac
     2701 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     2761 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     2821 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     2881 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     2941 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     3001 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3061 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     3121 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
     3181 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3241 ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3301 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     3361 aggtcgacga agactttctg tacagcacca tagatcaacg acattactat atatataata
     3421 taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt atgacgccag
     3481 atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac gtacaatctt
     3541 gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa aagaaaagga
     3601 gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt gattaagcac
     3661 acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg tccattggtg
     3721 aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc cgatgctgac
     3781 ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt tattgcaagg
     3841 aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa atacttggtt
     3901 ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga ttacggcatt
     3961 gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt cctcggtttg
     4021 ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag tgcagcttca
     4081 cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac aggtgaactt
     4141 ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga aagcttacat
     4201 tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag attaaatggc
     4261 gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga ctctaacaaa
     4321 atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta tttatttaag
     4381 tattgtttgt gcacttgccg atctatgcgg tgtgaaatac gagtaacgaa gcatctgtgc
     4441 ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     4501 gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct
     4561 gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     4621 tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag
     4681 aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa
     4741 agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact
     4801 ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc
     4861 cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc
     4921 attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact
     4981 ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt
     5041 tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc
     5101 gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat
     5161 aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg
     5221 gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt
     5281 ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt
     5341 ttttgaaagt gcgtcatcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac
     5401 tttctagcta gagaatagga acttc
//
