LOCUS       62056_23065        5813 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast LEU LIII A-B, complete sequence.
ACCESSION   MK495794
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 5813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..5813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(43..855)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1080..1668
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1938..1968
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2022..2678
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3023..3325
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     promoter        3625..4032
                     /label=LEU2 promoter
     CDS             4033..5124
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     regulatory      5128..5301
                     /label=AgLEU2 Terminator
                     /note="AgLEU2 Terminator"
                     /regulatory_class="terminator"
     misc_feature    5306..5809
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgcccctt acgttgtctt caaagctgaa
     1801 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     1861 actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct cgaggggccg
     1921 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     1981 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2041 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2101 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2161 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2221 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2281 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2341 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     2401 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag
     2461 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     2521 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     2581 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     2641 aattacaaca gtactgcgat gagtggcagg gcggggcgta atctagagga tccggcttac
     2701 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     2761 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     2821 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     2881 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     2941 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     3001 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3061 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     3121 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
     3181 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3241 ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3301 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     3361 aggtcgacga agactttctg tacatcgagg agaacttcta gtatatctac atacctaata
     3421 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa
     3481 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa
     3541 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat
     3601 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga
     3661 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg
     3721 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg
     3781 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag agcgccggaa
     3841 ccggcttttc atatagaata gagaagcgtt catgactaaa tgcttgcatc acaatacttg
     3901 aagttgacaa tattatttaa ggacctattg ttttttccaa taggtggtta gcaatcgtct
     3961 tactttctaa cttttcttac cttttacatt tcagcaatat atatatatat atttcaagga
     4021 tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
     4081 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat
     4141 gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt
     4201 ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt gttaggtgct
     4261 gtgggtggtc ctaaatgggg aaccggtagt gttagacctg aacaaggttt actaaaaatc
     4321 cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt
     4381 ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga
     4441 gaattagtgg gaggtattta ctttggtaag agaaaggagg acgatggtga tggtgtcgct
     4501 tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc
     4561 atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg
     4621 gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga atttcctaca
     4681 ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc
     4741 cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
     4801 tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac
     4861 aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag
     4921 aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg
     4981 aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt
     5041 atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
     5101 gaagaagtta agaaaatcct tgcttaacct ggaggaccct tctctttaga ctattctact
     5161 cttatgcacg taaaaaattc taggaaatat gtattaacta ggagtaaaat aaccggctag
     5221 tggcattcat atagccgtct gtttacatct acatcacaca tttcgagtgt atatctcgca
     5281 acgttggcgt taaataggca ggagtatcac gtgctataaa aataattata atttaaattt
     5341 tttaatataa atatataaat taaaaataga aagtaaaaaa agaaattaaa gaaaaaatag
     5401 tttttgtttt ccgaagatgt aaaagactct agggggatcg ccaacaaata ctacctttta
     5461 tcttgctctt cctgctctca ggtattaatg ccgaattgtt tcatcttgtc tgtgtagaaa
     5521 accacacacg aaaatcctgt gattttacat tttacttatc gttaatcgaa tgtatatcta
     5581 tttaatctgc ttttcttgtc taataaatat atatgtaaag tacgcttttt gttgaaattt
     5641 tttaaacctt tgtttatttt tttttcttca ttccgtaact cttctacctt ctttatttac
     5701 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
     5761 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtc
//
