LOCUS       62056_23060        5291 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast KAN LIII A-B, complete sequence.
ACCESSION   MK495797
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(43..855)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1080..1668
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1938..1968
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2022..2678
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3023..3325
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            3424..4779
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     misc_feature    4784..5287
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgcccctt acgttgtctt caaagctgaa
     1801 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     1861 actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct cgaggggccg
     1921 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     1981 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2041 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2101 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2161 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2221 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2281 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2341 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     2401 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag
     2461 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     2521 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     2581 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     2641 aattacaaca gtactgcgat gagtggcagg gcggggcgta atctagagga tccggcttac
     2701 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     2761 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     2821 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     2881 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     2941 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     3001 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3061 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     3121 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
     3181 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3241 ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3301 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     3361 aggtcgacga agactttctg tacagtttag cttgcctcgt ccccgccggg tcacccggcc
     3421 agcgacatgg aggcccagaa taccctcctt gacagtcttg acgtgcgcag ctcaggggca
     3481 tgatgtgact gtcgcccgta catttagccc atacatcccc atgtataatc atttgcatcc
     3541 atacattttg atggccgcac ggcgcgaagc aaaaattacg gctcctcgct ccagacctgc
     3601 gagcagggaa acgctcccct cacagacgcg ttgaattgtc cccacgccgc gcccctgtag
     3661 agaaatataa aaggttagga tttgccactg aggttcttct ttcatatact tccttttaaa
     3721 atcttgctag gatacagttc tcacatcaca tccgaacata aacaaaaatg ggtaaggaaa
     3781 agactcacgt ttcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     3841 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
     3901 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     3961 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     4021 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggc aaaacagcat
     4081 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     4141 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     4201 ttcgtctggc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     4261 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
     4321 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     4381 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     4441 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     4501 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     4561 tcgatgagtt tttctaaagt aactgacaat aaaaagattc ttgttttcaa gaacttgtca
     4621 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
     4681 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
     4741 tcatgcgtca atcgtatgtg aatgctggtc gctatactgg agtatcacgt gctataaaaa
     4801 taattataat ttaaattttt taatataaat atataaatta aaaatagaaa gtaaaaaaag
     4861 aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag ggggatcgcc
     4921 aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc gaattgtttc
     4981 atcttgtctg tgtagaaaac cacacacgaa aatcctgtga ttttacattt tacttatcgt
     5041 taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat atgtaaagta
     5101 cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt ccgtaactct
     5161 tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata aataaacaca
     5221 gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa gttacaggca
     5281 agcgatccgt c
//
