LOCUS       62056_23055        5006 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast HIS LIII A-B, complete sequence.
ACCESSION   MK495795
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 5006)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..5006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(43..855)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1080..1668
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1938..1968
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2022..2678
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3023..3325
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     promoter        3434..3621
                     /label=HIS3 promoter
     CDS             3622..4281
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     regulatory      4285..4494
                     /label=HIS3 Terminator
                     /note="HIS3 Terminator"
                     /regulatory_class="terminator"
     misc_feature    4499..5002
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgcccctt acgttgtctt caaagctgaa
     1801 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     1861 actacataat actgtaaaac acaacatatc cagtcactat ggcggcccct cgaggggccg
     1921 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     1981 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     2041 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     2101 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     2161 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     2221 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     2281 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     2341 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     2401 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag
     2461 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     2521 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     2581 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     2641 aattacaaca gtactgcgat gagtggcagg gcggggcgta atctagagga tccggcttac
     2701 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     2761 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     2821 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     2881 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     2941 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     3001 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3061 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     3121 gatccccctg gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt
     3181 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3241 ttccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3301 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     3361 aggtcgacga agactttctg tacaaacaca gtcctttccc gcaattttct ttttctatta
     3421 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
     3481 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
     3541 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
     3601 gcaagataaa cgaaggcaaa gatgacagag cagaaagccc tagtaaagcg tattacaaat
     3661 gaaaccaaga ttcagattgc gatctcttta aagggtggtc ccctagcgat agagcactcg
     3721 atcttcccag aaaaagaggc agaagcagta gcagaacagg ccacacaatc gcaagtgatt
     3781 aacgtccaca caggtatagg gtttctggac catatgatac atgctctggc caagcattcc
     3841 ggctggtcgc taatcgttga gtgcattggt gacttacaca tagacgacca tcacaccact
     3901 gaggactgcg ggattgctct cggtcaagct tttaaagagg ccctaggggc cgtgcgtgga
     3961 gtaaaaaggt ttggatcagg atttgcgcct ttggatgagg cactttccag agcggtggtt
     4021 gatctttcga acaggccgta cgcagttgtc gaacttggtt tgcaaaggga gaaagtaggt
     4081 gatctctctt gcgagatgat cccgcatttt cttgaaagct ttgcagaggc tagcagaatt
     4141 accctccacg ttgattgtct gcgaggcaag aatgatcatc accgtagtga gagtgcgttc
     4201 aaggctcttg cggttgccat aagagaagcc acctcgccca atggtaccaa cgatgttccc
     4261 tccaccaaag gtgttcttat gtagtgacac cgattattta aagctgctgc atacgatata
     4321 tatacatgtg tatatatgta tacctatgaa tgtcagtaag tatgtatacg aacagtatga
     4381 tactgaagat gacaaggtaa tgcatcattc tatacgtgtc attctgaacg aggcgcgctt
     4441 tccttttttc tttttgcttt ttcttttttt ttctcttgaa ctcgacggat catagagtat
     4501 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
     4561 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
     4621 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
     4681 atgccgaatt gtttcatctt gtctgtgtag aaaaccacac acgaaaatcc tgtgatttta
     4741 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
     4801 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
     4861 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
     4921 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
     4981 tgtaagttac aggcaagcga tccgtc
//
