LOCUS       62056_23050        5190 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast CEN LII F5-6, complete sequence.
ACCESSION   MK495793
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 5190)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..5190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..508
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     CDS             complement(528..1316)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      1746..2334
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2634..2664
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2718..3374
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3719..4021
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      4132..4308
                     /label=ScURA3 Promoter
                     /note="ScURA3 Promoter"
                     /regulatory_class="promoter"
     CDS             4309..5109
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      5113..5190
                     /label=ScURA3 Terminator
                     /note="ScURA3 Terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 gagtatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa tatataaatt
       61 aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc cgaagatgta
      121 aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc ctgctctcag
      181 gtattaatgc cgaattgttt catcttgtct gtgtagaaaa ccacacacga aaatcctgtg
      241 attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct tttcttgtct
      301 aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt gtttattttt
      361 ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc caaatacaaa
      421 acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta aaagatacga
      481 ggcgcgtgta agttacaggc aagcgatccg tcccgagcgg ccgcttattt gccgactacc
      541 ttggtgatct cgcctttcac gtagtggaca aattcttcca actgatctgc gcgcgaggcc
      601 aagcgatctt cttcttgtcc aagataagcc tgtctagctt caagtatgac gggctgatac
      661 tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg
      721 gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc
      781 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
      841 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
      901 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagataccc
      961 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
     1021 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
     1081 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
     1141 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
     1201 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
     1261 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcataatg
     1321 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
     1381 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
     1441 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     1501 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
     1561 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
     1621 ccggcaacct tgggtagcag cgaagtcgag gcatttctgt cctggctggt catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtgcggccg cccctgaatt cgcatctaga
     2461 ctgagaagac aatgaggcgg tgagaccaaa gctgaacgag aaacgtaaaa tgatataaat
     2521 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     2581 catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca ggctttacac
     2641 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     2701 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     2761 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     2821 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     2881 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     2941 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     3001 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     3061 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     3121 ctaaagggtt tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca
     3181 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     3241 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     3301 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     3361 ggcagggcgg ggcgtaatct agaggatccg gcttactaaa agccagataa cagtatgcgt
     3421 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     3481 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     3541 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     3601 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     3661 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     3721 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     3781 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     3841 gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     3901 gcgcatgatg accaccgata tggccagtgt gccggtttcc gttatcgggg aagaagtggc
     3961 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     4021 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgacggtctc atgtctctgt
     4081 tgtcttcagc agttacagag atgttacgaa ccactagtgc actgcagtac acggtttcct
     4141 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     4201 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     4261 ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct
     4321 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     4381 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     4441 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     4501 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     4561 aattttttac tcttcgagga cagaaaattt gctgacattg gtaatacagt caaattgcag
     4621 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     4681 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     4741 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     4801 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     4861 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     4921 ggtttagatg acaagggaga tgcattgggt caacagtata gaaccgtgga tgatgtggtt
     4981 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     5041 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     5101 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     5161 gcttcaattt aattatatca gttattaccc
//
