LOCUS       62056_23025        5683 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast 2micron LII F5-6, complete sequence.
ACCESSION   MK495788
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 5683)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..5683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..804)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      1234..1822
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2122..2152
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2206..2862
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3207..3509
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     regulatory      3620..3796
                     /label=ScURA3 Promoter
                     /note="ScURA3 Promoter"
                     /regulatory_class="promoter"
     CDS             3797..4597
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      4601..4678
                     /label=ScURA3 Terminator
                     /note="ScURA3 Terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(4683..5665)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ccgagcggcc gcttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
       61 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
      121 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
      181 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
      241 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
      301 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
      361 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
      421 gtcgatcgtg gctggctcga agatacccgc aagaatgtca ttgcgctgcc attctccaaa
      481 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
      541 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
      601 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
      661 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
      721 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
      781 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
      841 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
      901 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
      961 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     1021 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
     1081 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
     1141 atttctgtcc tggctggtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1381 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1921 tgcggccgcc cctgaattcg catctagact gagaagacaa tgaggcggtg agaccaaagc
     1981 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     2041 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc ccctcgaggg
     2101 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     2161 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2221 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     2281 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2341 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2401 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     2461 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2521 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2581 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtg
     2641 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2701 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2761 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2821 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatctag aggatccggc
     2881 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     2941 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     3001 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     3061 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     3121 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     3181 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     3241 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     3301 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt gaactttacc
     3361 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     3421 cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     3481 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     3541 ctgcaggtcg acggtctcat gtctctgttg tcttcagcag ttacagagat gttacgaacc
     3601 actagtgcac tgcagtacac ggtttccttg aaattttttt gattcggtaa tctccgaaca
     3661 gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt
     3721 tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacgtgcag
     3781 gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag
     3841 tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc
     3901 attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat
     3961 ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa
     4021 gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaggaca gaaaatttgc
     4081 tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg
     4141 ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca
     4201 ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg
     4261 caagggctcc ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga
     4321 caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta
     4381 cgattggttg attatgacac ccggtgtggg tttagatgac aagggagatg cattgggtca
     4441 acagtataga accgtggatg atgtggtttc tacaggatct gacattatta ttgttggaag
     4501 aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg
     4561 ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa
     4621 tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattacccga
     4681 gtaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt
     4741 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat
     4801 tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct
     4861 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc
     4921 gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga
     4981 gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa
     5041 tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt
     5101 ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac
     5161 tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg
     5221 atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc
     5281 agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta
     5341 cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta
     5401 aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa
     5461 ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga
     5521 gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag
     5581 tccggtgcgt ttttggtttt ttgaaagtgc gtcatcagag cgcttttggt tttcaaaagc
     5641 gctctgaagt tcctatactt tctagctaga gaataggaac ttc
//
