LOCUS       62056_23005        6541 bp DNA     circular SYN 29-JUL-2019
DEFINITION  Cloning vector pYeast 2micron LII F1-2, complete sequence.
ACCESSION   MK495784
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Chiasson D, Gimenez-Oya V, Bircheneder M, Bachmaier S, Studtrucker 
            T, Ryan J, Sollweck K, Leonhardt H, Boshart M, Dietrich P, Parniske 
            M.
  TITLE     A unified multi-kingdom Golden Gate cloning platform
  JOURNAL   Sci Rep 9 (1), 10131 (2019)
  PUBMED    31300661
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Chiasson D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4,
            Martinsried 82152, Germany
REFERENCE   3  (bases 1 to 6541)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2019"; volume: "9"; issue: "1"; pages: "10131"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-FEB-2019) Biology, LMU Munich, Grosshaderner Str. 2-4, 
            Martinsried 82152, Germany"
FEATURES             Location/Qualifiers
     source          1..6541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(16..804)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      1234..1822
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2122..2152
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2206..2862
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3207..3509
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     promoter        3860..4267
                     /label=LEU2 promoter
     CDS             4268..5359
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     regulatory      5363..5536
                     /label=AgLEU2 Terminator
                     /note="AgLEU2 Terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(5541..6523)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ccgagcggcc gcttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
       61 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
      121 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
      181 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
      241 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
      301 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
      361 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
      421 gtcgatcgtg gctggctcga agatacccgc aagaatgtca ttgcgctgcc attctccaaa
      481 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
      541 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
      601 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
      661 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
      721 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
      781 ttcggcgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
      841 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
      901 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac
      961 cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     1021 catacgctac ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc
     1081 cttcatccgt ttccacggtg tgcgtcaccc ggcaaccttg ggtagcagcg aagtcgaggc
     1141 atttctgtcc tggctggtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1381 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1921 tgcggccgcc cctgaattcg catctagact gagaagacaa tacggcggtg agaccaaagc
     1981 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
     2041 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc ccctcgaggg
     2101 gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt
     2161 ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     2221 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     2281 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     2341 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     2401 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     2461 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     2521 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     2581 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtg
     2641 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     2701 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     2761 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     2821 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatctag aggatccggc
     2881 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     2941 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
     3001 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     3061 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     3121 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     3181 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     3241 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     3301 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt gaactttacc
     3361 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     3421 cggtttccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     3481 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
     3541 ctgcaggtcg acggtctcat gtcgggcttg tcttcagcag ttacagagat gttacgaacc
     3601 actagtgcac tgcagtacat cgaggagaac ttctagtata tctacatacc taatattatt
     3661 gccttattaa aaatggaatc ccaacaatta catcaaaatc cacattctct tcaaaatcaa
     3721 ttgtcctgta cttccttgtt catgtgtgtt caaaaacgtt atatttatag gataattata
     3781 ctctatttct caacaagtaa ttggttgttt ggccgagcgg tctaaggcgc ctgattcaag
     3841 aaatatcttg accgcagtta actgtgggaa tactcaggta tcgtaagatg caagagttcg
     3901 aatctcttag caaccattat ttttttcctc aacataacga gaacacacag gggcgctatc
     3961 gcacagaatc aaattcgatg actggaaatt ttttgttaat ttcagaggtc gcctgacgca
     4021 tatacctttt tcaactgaaa aattgggaga aaaaggaaag gtgagagcgc cggaaccggc
     4081 ttttcatata gaatagagaa gcgttcatga ctaaatgctt gcatcacaat acttgaagtt
     4141 gacaatatta tttaaggacc tattgttttt tccaataggt ggttagcaat cgtcttactt
     4201 tctaactttt cttacctttt acatttcagc aatatatata tatatatttc aaggatatac
     4261 cattctaatg tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga
     4321 aatcacagcc gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa
     4381 gttcgatttc gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgttccact
     4441 tccagatgag gcgctggaag cctccaagaa ggctgatgcc gttttgttag gtgctgtggg
     4501 tggtcctaaa tggggaaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa
     4561 agaacttcaa ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga
     4621 cttatctcca atcaagccac aatttgctaa aggtactgac ttcgttgttg tcagagaatt
     4681 agtgggaggt atttactttg gtaagagaaa ggaggacgat ggtgatggtg tcgcttggga
     4741 tagtgaacaa tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc
     4801 cctacaacat gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc
     4861 ttcaagatta tggagaaaaa ctgtggagga aaccatcaag aacgaatttc ctacattgaa
     4921 ggttcaacat caattgattg attctgccgc catgatccta gttaagaacc caacccacct
     4981 aaatggtatt ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt
     5041 tatcccaggt tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa
     5101 caccgcattt ggtttgtacg aaccatgcca cggttctgct ccagatttgc caaagaataa
     5161 ggtcaaccct atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt
     5221 gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag gttttggatg caggtatcag
     5281 aactggtgat ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga
     5341 agttaagaaa atccttgctt aacctggagg acccttctct ttagactatt ctactcttat
     5401 gcacgtaaaa aattctagga aatatgtatt aactaggagt aaaataaccg gctagtggca
     5461 ttcatatagc cgtctgttta catctacatc acacatttcg agtgtatatc tcgcaacgtt
     5521 ggcgttaaat aggcaggagt aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     5581 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     5641 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
     5701 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     5761 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
     5821 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
     5881 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
     5941 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
     6001 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
     6061 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
     6121 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
     6181 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
     6241 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
     6301 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
     6361 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
     6421 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt catcagagcg
     6481 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagctagaga ataggaactt
     6541 c
//
