LOCUS       40924_48003        3062 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Measurement vector pYeaR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 3062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..122
                     /label=pYeaR
                     /note="pYeaR"
                     /regulatory_class="promoter"
     misc_RNA        136..186
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      208..235
                     /label=JBEI RBS
                     /note="JBEI RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             236..949
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    956..976
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      977..1036
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(1287..1875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1963..2057)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2081..2737)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2738..2840)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3017..3060)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agttcccatc tataatcctc cctgattctt cgctgatatg
       61 gtgctaaaaa gtaaccaata aatggtattt aaaatgcaaa ttatcaggcg taccctgaaa
      121 cgtgcatgca tgcaagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg
      181 aaacagcctc tacaaataat tttgtttaaa gatcttttaa gaaggagata tacatatgcg
      241 taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg atggtgatgt
      301 caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta atggtaaact
      361 gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga ctctggtaac
      421 gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga agcagcatga
      481 cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt cctttaagga
      541 tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc tggtaaaccg
      601 cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc ataagctgga
      661 atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa atggcattaa
      721 agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg ctgatcacta
      781 ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc actatctgag
      841 cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg ttctgctgga
      901 gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat gatgatacta
      961 gtagcggccg ctgcagtccg gcaaaaaaac gggcaaggtg tcaccaccct gccctttttc
     1021 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     1081 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tcctagctag
     1141 ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1201 gaatcagggg nnnacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1261 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1321 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1381 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1441 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     1501 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1561 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1621 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1681 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     1741 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1801 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1861 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1921 gaaaactcac gttaagggat tttggtcatg acttgtgctt ggattctcac caataaaaaa
     1981 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     2041 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     2101 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat
     2161 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2221 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2281 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2341 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2401 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2461 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     2521 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2581 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2641 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2701 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2761 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2821 aacctcttac gtgccgatca acgtctcatt ttcgccagat atcgacgtct aagaaaccat
     2881 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct
     2941 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     3001 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     3061 tg
//
