LOCUS       40924_47998        3831 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Controller 3 vector pYeaR-TP901-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 3831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..122
                     /label=pYeaR
                     /note="pYeaR"
                     /regulatory_class="promoter"
     misc_RNA        136..186
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             261..1724
                     /codon_start=1
                     /product="TP901-1 integrase"
                     /label=TP901-1 integrase
                     /protein_id="AKC96466.1"
                     /translation="MTKKVAIYTRVSTTNQAEEGFSIDEQIDRLTKYAEAMGWQVSDTY
                     TDAGFSGAKLERPAMQRLINDIENKAFDTVLVYKLDRLSRSVRDTLYLVKDVFTKNKID
                     FISLNESIDTSSAMGSLFLTILSAINEFERENIKERMTMGKLGRAKSGKSMMWTKTAFG
                     YYHNRKTGILEIVPLQATIVEQIFTDYLSGISLTKLRDKLNESGHIGKDIPWSYRTLRQ
                     TLDNPVYCGYIKFKDSLFEGMHKPIIPYETYLKVQKELEERQQQTYERNNNPRPFQAKY
                     MLSGMARCGYCGAPLKIVLGHKRKDGSRTMKYHCANRFPRKTKGITVYNDNKKCDSGTY
                     DLSNLENTVIDNLIGFQENNDSLLKIINGNNQPILDTSSFKKQISQIDKKIQKNSDLYL
                     NDFITMDELKDRTDSLQAEKKLLKAKISENKFNDSTDVFELVKTQLGSIPINELSYDNK
                     KKIVNNLVSKVDVTADNVDIIFKFQLATG"
     misc_feature    1725..1745
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1746..1805
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2056..2644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2732..2826)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2850..3506)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3507..3609)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3786..3829)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agttcccatc tataatcctc cctgattctt cgctgatatg
       61 gtgctaaaaa gtaaccaata aatggtattt aaaatgcaaa ttatcaggcg taccctgaaa
      121 cgtgcatgca tgcaagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg
      181 aaacagcctc tacaaataat tttgtttaac ccaagcttgg gtactagaga agcttgcggc
      241 tttcacacga gggggctagc gtgactaaga aagtagcaat ctatacacga gtatccacta
      301 ctaaccaagc agaggaaggc ttctcaattg atgagcaaat tgaccgttta acaaaatatg
      361 ctgaagcaat ggggtggcaa gtatctgata cttatactga tgctggtttt tcaggggcca
      421 aacttgaacg cccagcaatg caaagattaa tcaacgatat cgagaataaa gcttttgata
      481 cagttcttgt atataagcta gaccgccttt cacgtagtgt aagagatact ctttatcttg
      541 ttaaggatgt gttcacaaaa aataaaatag actttatctc gcttaatgaa agtattgata
      601 cttcttctgc tatgggtagc ttgtttctca ctattctttc tgcaattaat gagtttgaaa
      661 gagagaatat aaaagaacgc atgactatgg gtaaactagg gcgagcgaaa tctggtaagt
      721 ctatgatgtg gactaagaca gcttttgggt attaccacaa cagaaagaca ggtatattag
      781 aaattgttcc tttacaagct acaatagttg aacaaatatt cactgattat ttatcaggaa
      841 tatcacttac aaaattaaga gataaactca atgaatctgg acacatcggt aaagatatac
      901 cgtggtctta tcgtacccta agacaaacac ttgataatcc agtttactgt ggttatatca
      961 aatttaagga cagcctattt gaaggtatgc acaaaccaat tatcccttat gagacttatt
     1021 taaaagttca aaaagagcta gaagaaagac aacagcagac ttatgaaaga aataacaacc
     1081 ctagaccttt ccaagctaaa tatatgctgt cagggatggc aaggtgcggt tactgtggag
     1141 cacctttaaa aattgttctt ggccacaaaa gaaaagatgg aagccgcact atgaaatatc
     1201 actgtgcaaa tagatttcct cgaaaaacaa aaggaattac agtatataat gacaataaaa
     1261 agtgtgattc aggaacttat gatttaagta atttagaaaa tactgttatt gacaacctga
     1321 ttggatttca agaaaataat gactccttat tgaaaattat caatggcaac aaccaaccta
     1381 ttcttgatac ttcgtcattt aaaaagcaaa tttcacagat cgataaaaaa atacaaaaga
     1441 actctgattt gtacctaaat gattttatca ctatggatga gttgaaagat cgtactgatt
     1501 cccttcaggc tgagaaaaag ctgcttaaag ctaagattag cgaaaataaa tttaatgact
     1561 ctactgatgt ttttgagtta gttaaaactc agttgggctc aattccgatt aatgaactat
     1621 catatgataa taaaaagaaa atcgtcaaca accttgtatc aaaggttgat gttactgctg
     1681 ataatgtaga tatcatattt aaattccaac tcgctaccgg ttaatactag tagcggccgc
     1741 tgcagtccgg caaaaaaacg ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
     1801 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat cctagctagg gcgttcggct
     1921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcaggggn
     1981 nnacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2221 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     2281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2461 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     2521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2701 ttaagggatt ttggtcatga cttgtgcttg gattctcacc aataaaaaac gcccggcggc
     2761 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     2821 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     2881 tgtaattcat taagcattct gccgacatgg aagccatcac agacggcatg atgaacctga
     2941 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     3001 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     3061 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     3121 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     3181 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     3241 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     3301 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     3361 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     3421 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     3481 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     3541 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     3601 tgccgatcaa cgtctcattt tcgccagata tcgacgtcta agaaaccatt attatcatga
     3661 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagtgccacc
     3721 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3781 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct g
//
