LOCUS       40924_47993        3895 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Controller 1 vector pYeaR-Bxb1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 3895)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3895)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..122
                     /label=pYeaR
                     /note="pYeaR"
                     /regulatory_class="promoter"
     misc_RNA        136..186
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             244..1785
                     /codon_start=1
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /protein_id="AKC96464.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMSTGAANDENYAAHL"
     misc_feature    1789..1809
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1810..1869
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2120..2708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2796..2890)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2914..3570)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3571..3673)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3850..3893)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agttcccatc tataatcctc cctgattctt cgctgatatg
       61 gtgctaaaaa gtaaccaata aatggtattt aaaatgcaaa ttatcaggcg taccctgaaa
      121 cgtgcatgca tgcaagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg
      181 aaacagcctc tacaaataat tttgtttaac ccaagcttgc ggctttcaca cgtggttgct
      241 agcatgagag ccctggtagt catccgcctg tcccgcgtca ccgatgctac gacttcaccg
      301 gagcgtcagc tggagtcttg ccagcagctc tgcgcccagc gcggctggga cgtcgtcggg
      361 gtagcggagg atctggacgt ctccggggcg gtcgatccgt tcgaccggaa gcgcagaccg
      421 aacctggccc ggtggctagc gttcgaggag caaccgtttg acgtgatcgt ggcgtaccgg
      481 gtagatcggt tgacccgatc gatccggcat cttcagcagc tggtccactg ggccgaggac
      541 cacaagaagc tggtcgtctc cgcgaccgaa gcgcacttcg atacgacgac gccgtttgcg
      601 gcggtcgtca tcgcgcttat gggaacggtg gcgcagatgg aattagaagc gatcaaagag
      661 cggaaccgtt cggctgcgca tttcaatatc cgcgccggga aataccgagg atccctgccg
      721 ccgtggggat acctgcctac gcgcgtggac ggggagtggc ggctggtgcc ggaccctgtg
      781 cagcgagagc gcatcctcga ggtgtatcac cgcgtcgtcg acaaccacga gccgctgcat
      841 ctggtggccc acgacctgaa ccggcgtggt gtcctgtcgc cgaaggacta cttcgcgcag
      901 ctgcaaggcc gcgagccgca gggccgggag tggtcggcta ccgcgctgaa gcgatcgatg
      961 atctccgagg cgatgctcgg gtacgcgact ctgaacggta agaccgtccg agacgacgac
     1021 ggagccccgc tggtgcgggc tgagccgatc ctgacccgtg agcagctgga ggcgctgcgc
     1081 gccgagctcg tgaagacctc ccgggcgaag cccgcggtgt ctaccccgtc gctgctgctg
     1141 cgggtgttgt tctgcgcggt gtgcggggag cccgcgtaca agttcgccgg gggaggacgt
     1201 aagcacccgc gctaccgctg ccgctcgatg gggttcccga agcactgcgg gaacggcacg
     1261 gtggcgatgg ccgagtggga cgcgttctgc gaggagcagg tactggatct gctcggggac
     1321 gcggagcgtc tggagaaagt ctgggtagcg ggctcggact ccgcggtcga actcgcggag
     1381 gtgaacgcgg agctggtgga cctgacgtcg ctgatcggct ccccggccta ccgggcgggc
     1441 tctccgcagc gagaagcact ggatgcccgt attgcggcgc tggccgcgcg gcaagaggag
     1501 ctggagggcc tggaggctcg cccgtctggc tgggagtggc gcgagaccgg gcagcggttc
     1561 ggggactggt ggcgggagca ggacaccgcg gcaaagaaca cctggcttcg gtcgatgaac
     1621 gttcggctga cgttcgacgt ccgcggcggg ctgactcgca cgatcgactt cggggatctt
     1681 caggagtacg agcagcatct caggctcggc agcgtggtcg aacggctaca caccgggatg
     1741 tcgaccggcg ctgctaacga cgaaaactac gctgctcatc tttaataata ctagtagcgg
     1801 ccgctgcagt ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa
     1861 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
     1921 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatcctagc tagggcgttc
     1981 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2041 gggnnnacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2101 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2221 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2281 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     2341 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2401 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2461 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2521 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     2581 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2641 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2701 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2761 cacgttaagg gattttggtc atgacttgtg cttggattct caccaataaa aaacgcccgg
     2821 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2881 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     2941 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac
     3001 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3061 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3121 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3181 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3241 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3301 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     3361 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     3421 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     3481 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3541 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3601 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     3661 tacgtgccga tcaacgtctc attttcgcca gatatcgacg tctaagaaac cattattatc
     3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtgc
     3781 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     3841 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctg
//
