LOCUS       40924_47988        4579 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Controller 1 vector pYeaR-Bxb1-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 4579)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4579)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..122
                     /label=pYeaR
                     /note="pYeaR"
                     /regulatory_class="promoter"
     misc_RNA        136..186
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             244..1746
                     /codon_start=1
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /protein_id="AKC96467.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     CDS             1771..2466
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     misc_feature    2473..2493
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2494..2553
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2804..3392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(3480..3574)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3598..4254)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4255..4357)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(4534..4577)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agttcccatc tataatcctc cctgattctt cgctgatatg
       61 gtgctaaaaa gtaaccaata aatggtattt aaaatgcaaa ttatcaggcg taccctgaaa
      121 cgtgcatgca tgcaagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg
      181 aaacagcctc tacaaataat tttgtttaac ccaagcttgc ggctttcaca cgtggttgct
      241 agcatgagag ccctggtagt catccgcctg tcccgcgtca ccgatgctac gacttcaccg
      301 gagcgtcagc tggagtcttg ccagcagctc tgcgcccagc gcggctggga cgtcgtcggg
      361 gtagcggagg atctggacgt ctccggggcg gtcgatccgt tcgaccggaa gcgcagaccg
      421 aacctggccc ggtggctagc gttcgaggag caaccgtttg acgtgatcgt ggcgtaccgg
      481 gtagatcggt tgacccgatc gatccggcat cttcagcagc tggtccactg ggccgaggac
      541 cacaagaagc tggtcgtctc cgcgaccgaa gcgcacttcg atacgacgac gccgtttgcg
      601 gcggtcgtca tcgcgcttat gggaacggtg gcgcagatgg aattagaagc gatcaaagag
      661 cggaaccgtt cggctgcgca tttcaatatc cgcgccggga aataccgagg atccctgccg
      721 ccgtggggat acctgcctac gcgcgtggac ggggagtggc ggctggtgcc ggaccctgtg
      781 cagcgagagc gcatcctcga ggtgtatcac cgcgtcgtcg acaaccacga gccgctgcat
      841 ctggtggccc acgacctgaa ccggcgtggt gtcctgtcgc cgaaggacta cttcgcgcag
      901 ctgcaaggcc gcgagccgca gggccgggag tggtcggcta ccgcgctgaa gcgatcgatg
      961 atctccgagg cgatgctcgg gtacgcgact ctgaacggta agaccgtccg agacgacgac
     1021 ggagccccgc tggtgcgggc tgagccgatc ctgacccgtg agcagctgga ggcgctgcgc
     1081 gccgagctcg tgaagacctc ccgggcgaag cccgcggtgt ctaccccgtc gctgctgctg
     1141 cgggtgttgt tctgcgcggt gtgcggggag cccgcgtaca agttcgccgg gggaggacgt
     1201 aagcacccgc gctaccgctg ccgctcgatg gggttcccga agcactgcgg gaacggcacg
     1261 gtggcgatgg ccgagtggga cgcgttctgc gaggagcagg tactggatct gctcggggac
     1321 gcggagcgtc tggagaaagt ctgggtagcg ggctcggact ccgcggtcga actcgcggag
     1381 gtgaacgcgg agctggtgga cctgacgtcg ctgatcggct ccccggccta ccgggcgggc
     1441 tctccgcagc gagaagcact ggatgcccgt attgcggcgc tggccgcgcg gcaagaggag
     1501 ctggagggcc tggaggctcg cccgtctggc tgggagtggc gcgagaccgg gcagcggttc
     1561 ggggactggt ggcgggagca ggacaccgcg gcaaagaaca cctggcttcg gtcgatgaac
     1621 gttcggctga cgttcgacgt ccgcggcggg ctgactcgca cgatcgactt cggggatctt
     1681 caggagtacg agcagcatct caggctcggc agcgtggtcg aacggctaca caccgggatg
     1741 tcgtaactag taaaaattaa gaggtatata ttaatgtcag aattaattaa agaaaatatg
     1801 cacatgaaat tatatatgga aggtactgtc aacaatcatc atttcaaatg cacatccgaa
     1861 ggtgaaggta aaccatatga aggcacacaa acaatgcgca tcaaagcagt tgaaggtgga
     1921 cccctgccct ttgcgtttga cattctcgca acgagcttta tgtacgggtc taaaactttt
     1981 atcaatcaca cccaaggcat tcctgacttt tttaaacagt cctttcctga aggctttacc
     2041 tgggaacgtg taacaactta tgaagatggc ggtgtactta cagcaactca agatacgagt
     2101 ttacaagatg gctgtctgat ttacaatgtt aaaatccgtg gcgtaaattt cccgagtaac
     2161 ggacccgtaa tgcaaaaaaa aactcttggt tgggaagcat caacagaaac cttatatcct
     2221 gcggacggtg gcttagaagg acgcgcagac atggcactga aattagttgg aggcggtcat
     2281 ttaatctgca acctgaaaac aacctatcgt tccaaaaaac ccgctaaaaa ccttaaaatg
     2341 cctggagtat actatgttga tcgtcgctta gaacgtatta aagaagctga taaagaaacc
     2401 tacgttgaac aacatgaagt agccgtagcc cgttattgtg accttccgtc gaaattagga
     2461 catcgttgat aatactagta gcggccgctg cagtccggca aaaaaacggg caaggtgtca
     2521 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
     2581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2641 gcggtaatcc tagctagggc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2701 taatacggtt atccacagaa tcaggggnnn acgcaggaaa gaacatgtga gcaaaaggcc
     2761 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2821 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2881 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2941 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     3001 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3061 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3121 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3181 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3241 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3301 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3361 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3421 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgact tgtgcttgga
     3481 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     3541 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     3601 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     3661 gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     3721 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     3781 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     3841 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     3901 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     3961 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     4021 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     4081 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     4141 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     4201 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     4261 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     4321 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccagatatc
     4381 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4441 ccctttcgtc ttcacctcga gtgccacctg acgtctaaga aaccattatt atcatgacat
     4501 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     4561 tcgctaagga tgatttctg
//
