LOCUS       40924_47488        8129 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYanni5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Graupner S, Wackernagel W.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Graupner S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet 
            Oldenburg, Postfach 2503, 26111 Oldenburg, GERMANY
REFERENCE   3  (bases 1 to 8129)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8129)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet Oldenburg,
            Postfach 2503, 26111 Oldenburg, GERMANY"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1153)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1154..1231)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1465..1493
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1501..1517
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1770..1856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1948..1975
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1994..2085
                     /label=AmpR promoter
     CDS             2086..2943
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3784..4407)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline repressor TetR"
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        4423..4478
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             4486..5688
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKTAVLLE
                     FIADSSAFAFLAFISEGWLDFPVLILLAGGGIALPALQGVMSIQTKSHEQGALQGLLVS
                     LTNATGVIGPLLFTVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     rep_origin      6094..6863
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6864..7523
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cgaacgccag caagacgtag cccagcgcgt cgcttgcaat tcgcgctaac ttacattaat
       61 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      181 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      241 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttgacg
      301 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      361 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      421 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      481 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      541 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      601 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
      661 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
      721 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
      781 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
      841 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
      901 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
      961 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1021 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1081 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1141 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1201 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gccgcttcgc cttcgcgcgc
     1261 gaattgcaag ctgatccggc ggagcttatc gactgcacgg tgcaccaatg cttctggcgt
     1321 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
     1381 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     1441 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagaattcc cggggatccg tcgacctgca
     1561 gccaagcttc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1621 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     1681 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1741 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1861 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     1921 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     1981 ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagc tggccgccac
     3361 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     3421 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     3481 actcaatgct tgagtaagct tgatgatctg atgattccct ttgtcaacag caatggatca
     3541 ctgaaaatgg ttcaatgatc acattaagtg gtattcaata ttttcatgaa atgggaattg
     3601 acgttccttc caaacattca cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc
     3661 gtttccattt aggtgggtac gttggagccg cattattttc gctttatgaa tctaaagggt
     3721 ggttaactcg acatcttggt taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt
     3781 ttaagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
     3841 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
     3901 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
     3961 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
     4021 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
     4081 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
     4141 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
     4201 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
     4261 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
     4321 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
     4381 tttactttta tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcattg
     4441 atagagttat tttaccactc cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca
     4501 aagatcgcat tggtaattac gttactcgat gccatgggga ttggccttat catgccagtc
     4561 ttgccaacgt tattacgtga atttattgct tcggaagata tcgctaacca ctttggcgta
     4621 ttgcttgcac tttatgcgtt aatgcaggtt atctttgctc cttggcttgg aaaaatgtct
     4681 gaccgatttg gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc gctggattac
     4741 ttattgctgg ctttttcaag tgcgctttgg atgctgtatt taggccgttt gctttcaggg
     4801 atcacaggag ctactggggc tgtcgcggca tcggtcattg ccgataccac ctcagcttct
     4861 caacgcgtga agtggttcgg ttggttaggg gcaagttttg ggcttggttt aatagcgggg
     4921 cctattattg gtggttttgc aggagagatt tcaccgcata gtcccttttt tatcgctgcg
     4981 ttgctaaata ttgtcacttt ccttgtggtt atgttttggt tccgtgaaac caaaaataca
     5041 cgtgataata cagataccga agtaggggtt gagacgcaat cgaattcggt atacatcact
     5101 ttatttaaaa cgatgcccat tttgttgatt atttattttt cagcgcaatt gataggccaa
     5161 attcccgcaa cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa tagcatgatg
     5221 gttggctttt cattagcggg tcttggtctt ttacactcag tattccaagc ctttgtggca
     5281 ggaagaatag ccactaaatg gggcgaaaaa acggcagtac tgctcgaatt tattgcagat
     5341 agtagtgcat ttgccttttt agcgtttata tctgaaggtt ggttagattt ccctgtttta
     5401 attttattgg ctggtggtgg gatcgcttta cctgcattac agggagtgat gtctatccaa
     5461 acaaagagtc atgagcaagg tgctttacag ggattattgg tgagccttac caatgcaacc
     5521 ggtgttattg gcccattact gtttactgtt atttataatc attcactacc aatttgggat
     5581 ggctggattt ggattattgg tttagcgttt tactgtatta ttatcctgct atcgatgacc
     5641 ttcatgttaa cccctcaagc tcaggggagt aaacaggaga caagtgctta gttatttcgt
     5701 caccaaatga tgttattccg cgaaatataa tgaccctctt gataacccaa gagggcattt
     5761 tttacgataa agaagattta gcttcaaata aaacctatct attttattta tctttcaagc
     5821 tcaataaaaa gccgcggtaa atagcaataa attggccttt tttatcggca agctctttta
     5881 ggtttttcgc atgtattgcg atatgcataa accagccatt gagtaagttt ttaagcacat
     5941 cactatcata agctttactc actagacttt gcttcgcaaa gtcgtgaccg cctacggcgg
     6001 ctgcggcgcc ctacgggctt gctctccggg cttcgccctg cgcggtcgct gcgctccctt
     6061 gccagcccgt ggatatgtgg acgatggccg cgagcggcca ccggctggct cgcttcgctc
     6121 ggcccgtgga caaccctgct ggacaagctg atggacaggc tgcgcctgcc cacgagcttg
     6181 accacaggga ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg
     6241 cgcggcctgc ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc
     6301 ctgcggcctg cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg
     6361 cgcagcgacc gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag
     6421 tgggggcagt cgaaggcgaa gcccgcccgc ctgccccccg agcctcacgg cggcgagtgc
     6481 gggggttcca agggggcagc gccaccttgg gcaaggccga aggccgcgca gtcgatcaac
     6541 aagccccgga ggggccactt tttgccggag ggggagccgc gccgaaggcg tgggggaacc
     6601 ccgcaggggt gcccttcttt gggcaccaaa gaactagata tagggcgaaa tgcgaaagac
     6661 ttaaaaatca acaacttaaa aaaggggggt acgcaacagc tcattgcggc accccccgca
     6721 atagctcatt gcgtaggtta aagaaaatct gtaattgact gccactttta cgcaacgcat
     6781 aattgttgtc gcgctgccga aaagttgcag ctgattgcgc atggtgccgc aaccgtgcgg
     6841 caccctaccg catggagata agcatggcca cgcagtccag agaaatcggc attcaagcca
     6901 agaacaagcc cggtcactgg gtgcaaacgg aacgcaaagc gcatgaggcg tgggccgggc
     6961 ttattgcgag gaaacccacg gcggcaatgc tgctgcatca cctcgtggcg cagatgggcc
     7021 accagaacgc cgtggtggtc agccagaaga cactttccaa gctcatcgga cgttctttgc
     7081 ggacggtcca atacgcagtc aaggacttgg tggccgagcg ctggatctcc gtcgtgaagc
     7141 tcaacggccc cggcaccgtg tcggcctacg tggtcaatga ccgcgtggcg tggggccagc
     7201 cccgcgacca gttgcgcctg tcggtgttca gtgccgccgt ggtggttgat cacgacgacc
     7261 aggacgaatc gctgttgggg catggcgacc tgcgccgcat cccgaccctg tatccgggcg
     7321 agcagcaact accgaccggc cccggcgagg agccgcccag ccagcccggc attccgggca
     7381 tggaaccaga cctgccagcc ttgaccgaaa cggaggaatg ggaacggcgc gggcagcagc
     7441 gcctgccgat gcccgatgag ccgtgttttc tggacgatgg cgagccgttg gagccgccga
     7501 cacgggtcac gctgccgcgc cggtagcact tgggttgcgc agcaacccgt aagtgcgctg
     7561 ttccagacta tcggctgtag ccgcctcgcc gccctatacc ttgtctgcct ccccgcgttg
     7621 cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
     7681 acggattcac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta
     7741 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca
     7801 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
     7861 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
     7921 cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta
     7981 aaaaaattac gccccgccct gccactcatc gcagtcggcc tattggttaa aaaatgagct
     8041 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttccattcg
     8101 ccattcaggc tgcgcaactg ttgggaagg
//
