LOCUS       40924_47483        7671 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYanni4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Graupner S, Wackernagel W.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Graupner S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet 
            Oldenburg, Postfach 2503, 26111 Oldenburg, GERMANY
REFERENCE   3  (bases 1 to 7671)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7671)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet Oldenburg,
            Postfach 2503, 26111 Oldenburg, GERMANY"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1153)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1154..1231)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1465..1493
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1501..1517
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1770..1856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1948..1975
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1994..2085
                     /label=AmpR promoter
     CDS             2086..2943
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             3771..4574
                     /codon_start=1
                     /gene="strA"
                     /product="streptomycin phosphotransferase"
                     /function="streptomycin resistance"
                     /label=strA
                     /protein_id="CAB46261.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            3771..4574
                     /gene="strA"
                     /label=strA
     CDS             4574..5410
                     /codon_start=1
                     /gene="strB"
                     /product="streptomycin resistance protein"
                     /label=strB
                     /protein_id="CAB46262.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            4574..5410
                     /gene="strB"
                     /label=strB
     rep_origin      5636..6405
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6406..7065
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cgaacgccag caagacgtag cccagcgcgt cgcttgcaat tcgcgctaac ttacattaat
       61 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      181 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      241 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttgacg
      301 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      361 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      421 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      481 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      541 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      601 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
      661 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
      721 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
      781 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
      841 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
      901 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
      961 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1021 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1081 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1141 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1201 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gccgcttcgc cttcgcgcgc
     1261 gaattgcaag ctgatccggc ggagcttatc gactgcacgg tgcaccaatg cttctggcgt
     1321 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
     1381 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     1441 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagaattcc cggggatccg tcgacctgca
     1561 gccaagcttc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1621 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     1681 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1741 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1861 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     1921 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     1981 ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagc tggccgccac
     3361 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     3421 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     3481 actcaatgct tgagtaaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg
     3541 ggaagccctg caaagtaaac tctgctggag taccacaact tcgccctgca gctgcagagc
     3601 ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc cccttgcgcg
     3661 acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc
     3721 gggatatttc tctatattcg cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa
     3781 ctaatatttt ttttggtgaa tcgcattctg actggttgcc tgtcagaggc ggagaatctg
     3841 gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc
     3901 gccgcggtga gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg
     3961 cttgccccga ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg gtgataacgg
     4021 caattccggg agtaccggcg gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt
     4081 caatggggca gcaacttggc gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc
     4141 gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc
     4201 ccgacttctt accggacgag gacaagagta cgccgctgca cgatcttttg gctcgtgtcg
     4261 aacgagagct accggtgcgg ctcgaccaag agcgcaccga tatggttgtt tgccatggtg
     4321 atccctgcat gccgaacttc atggtggacc ctaaaactct tcaatgcacg ggtctgatcg
     4381 accttgggcg gctcggaaca gcagatcgct atgccgattt ggcactcatg attgctaacg
     4441 ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg
     4501 tattggggat cgaagccccc gaccgcgaac gccttgcctt ctatctgcga ttggaccctc
     4561 tgacttgggg ttgatgttca tgccgcctgt ttttcctgct cattggcacg tttcgcaacc
     4621 tgttctcatt gcggacacct tttccagcct cgtttggaaa gtttcattgc cagacgggac
     4681 tcctgcaatc gtcaagggat tgaaacctat agaagacatt gctgatgaac tgcgcggggc
     4741 cgactatctg gtatggcgca atgggagggg agcagtccgg ttgctcggtc gtgagaacaa
     4801 tctgatgttg ctcgaatatg ccggggagcg aatgctctct cacatcgttg ccgagcacgg
     4861 cgactaccag gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg ccgcatctga
     4921 ggaacccctg ccttctgccc ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg
     4981 ggcgcgcgat gatcaaaacg caggttgtca aactgactac gtccacgcgg cgattatagc
     5041 cgatcaaatg atgagcaatg cctcggaact gcgtgggcta catggcgatc tgcatcatga
     5101 aaacatcatg ttctccagtc gcggctggct ggtgatagat cccgtcggtc tggtcggtga
     5161 agtgggcttt ggcgccgcca atatgttcta cgatccggct gacagagacg acctttgtct
     5221 cgatcctaga cgcattgcac agatggcgga cgcattctct cgtgcgctgg acgtcgatcc
     5281 gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga
     5341 tggagaagag gagcaacgcg atctagctat cgcggccgcg atcaagcagg tgcgacagac
     5401 gtcatactag atatcaagcg acttctccta tcccctggga acacatcaat ctcaccggag
     5461 aatatcgctg gccaaagcct tagcgtagga ttccgcctac tcactagact ttgcttcgca
     5521 aagtcgtgac cgcctacggc ggctgcggcg ccctacgggc ttgctctccg ggcttcgccc
     5581 tgcgcggtcg ctgcgctccc ttgccagccc gtggatatgt ggacgatggc cgcgagcggc
     5641 caccggctgg ctcgcttcgc tcggcccgtg gacaaccctg ctggacaagc tgatggacag
     5701 gctgcgcctg cccacgagct tgaccacagg gattgcccac cggctaccca gccttcgacc
     5761 acatacccac cggctccaac tgcgcggcct gcggccttgc cccatcaatt tttttaattt
     5821 tctctgggga aaagcctccg gcctgcggcc tgcgcgcttc gcttgccggt tggacaccaa
     5881 gtggaaggcg ggtcaaggct cgcgcagcga ccgcgcagcg gcttggcctt gacgcgcctg
     5941 gaacgaccca agcctatgcg agtgggggca gtcgaaggcg aagcccgccc gcctgccccc
     6001 cgagcctcac ggcggcgagt gcgggggttc caagggggca gcgccacctt gggcaaggcc
     6061 gaaggccgcg cagtcgatca acaagccccg gaggggccac tttttgccgg agggggagcc
     6121 gcgccgaagg cgtgggggaa ccccgcaggg gtgcccttct ttgggcacca aagaactaga
     6181 tatagggcga aatgcgaaag acttaaaaat caacaactta aaaaaggggg gtacgcaaca
     6241 gctcattgcg gcaccccccg caatagctca ttgcgtaggt taaagaaaat ctgtaattga
     6301 ctgccacttt tacgcaacgc ataattgttg tcgcgctgcc gaaaagttgc agctgattgc
     6361 gcatggtgcc gcaaccgtgc ggcaccctac cgcatggaga taagcatggc cacgcagtcc
     6421 agagaaatcg gcattcaagc caagaacaag cccggtcact gggtgcaaac ggaacgcaaa
     6481 gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca cggcggcaat gctgctgcat
     6541 cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg tcagccagaa gacactttcc
     6601 aagctcatcg gacgttcttt gcggacggtc caatacgcag tcaaggactt ggtggccgag
     6661 cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg tgtcggccta cgtggtcaat
     6721 gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc
     6781 gtggtggttg atcacgacga ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc
     6841 atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg gccccggcga ggagccgccc
     6901 agccagcccg gcattccggg catggaacca gacctgccag ccttgaccga aacggaggaa
     6961 tgggaacggc gcgggcagca gcgcctgccg atgcccgatg agccgtgttt tctggacgat
     7021 ggcgagccgt tggagccgcc gacacgggtc acgctgccgc gccggtagca cttgggttgc
     7081 gcagcaaccc gtaagtgcgc tgttccagac tatcggctgt agccgcctcg ccgccctata
     7141 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
     7201 ggaagccggc ggcacctcgc taacggattc accgttttta tcaggctctg ggaggcagaa
     7261 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7321 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7381 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7441 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7501 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtcgg
     7561 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7621 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag g
//
