LOCUS       40924_47478        7535 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYanni3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7535)
  AUTHORS   Graupner S, Wackernagel W.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7535)
  AUTHORS   Graupner S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet 
            Oldenburg, Postfach 2503, 26111 Oldenburg, GERMANY
REFERENCE   3  (bases 1 to 7535)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7535)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet Oldenburg,
            Postfach 2503, 26111 Oldenburg, GERMANY"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1153)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1154..1231)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1465..1493
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1501..1517
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1770..1856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1948..1975
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1994..2085
                     /label=AmpR promoter
     CDS             2086..2943
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(4215..5006)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      5500..6269
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             6270..6929
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cgaacgccag caagacgtag cccagcgcgt cgcttgcaat tcgcgctaac ttacattaat
       61 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      181 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      241 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttgacg
      301 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      361 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      421 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      481 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      541 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      601 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
      661 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
      721 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
      781 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
      841 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
      901 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
      961 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1021 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1081 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1141 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1201 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gccgcttcgc cttcgcgcgc
     1261 gaattgcaag ctgatccggc ggagcttatc gactgcacgg tgcaccaatg cttctggcgt
     1321 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
     1381 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     1441 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagaattcc cggggatccg tcgacctgca
     1561 gccaagcttc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1621 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     1681 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1741 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1861 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     1921 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     1981 ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagc tggccgccac
     3361 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     3421 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     3481 actcaatgct tgagtagatc ctgcaagcct cgtcgtcctg gccggaccac gctatctgtg
     3541 caaggtcccc ggccccggac gcgcgctcca tgagcagagc gcccgccgcc gaggcgaaga
     3601 ctcgggcggc gccctgcccg tcccaccagg tcaacaggcg gtaaccggcc tcttcatcgg
     3661 gaatgcgcgc gaccttcagc atcgccggca tgtccccctg gcggacggga agtatccagc
     3721 tcgagtgggt ggtgagcagc tcgccgtcgc gcagcaggcg ccagcgctcc atccacgcag
     3781 tgacctcagg cggaaaacgg gaagacacac tcatgagatg cctgcaagca attcgttctg
     3841 tatcaggcgc aggagcgtcc cgtccgggtc gaccaaagcg gccatcgtgc ctccccactc
     3901 ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
     3961 tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
     4021 cgcgagggga tcgagcccgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     4081 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     4141 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc
     4201 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     4261 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     4321 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     4381 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     4441 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     4501 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     4561 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     4621 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     4681 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     4741 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     4801 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     4861 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     4921 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     4981 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     5041 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     5101 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     5161 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     5221 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     5281 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     5341 tgcttgcggc agcgtgaagc ttactcacta gactttgctt cgcaaagtcg tgaccgccta
     5401 cggcggctgc ggcgccctac gggcttgctc tccgggcttc gccctgcgcg gtcgctgcgc
     5461 tcccttgcca gcccgtggat atgtggacga tggccgcgag cggccaccgg ctggctcgct
     5521 tcgctcggcc cgtggacaac cctgctggac aagctgatgg acaggctgcg cctgcccacg
     5581 agcttgacca cagggattgc ccaccggcta cccagccttc gaccacatac ccaccggctc
     5641 caactgcgcg gcctgcggcc ttgccccatc aattttttta attttctctg gggaaaagcc
     5701 tccggcctgc ggcctgcgcg cttcgcttgc cggttggaca ccaagtggaa ggcgggtcaa
     5761 ggctcgcgca gcgaccgcgc agcggcttgg ccttgacgcg cctggaacga cccaagccta
     5821 tgcgagtggg ggcagtcgaa ggcgaagccc gcccgcctgc cccccgagcc tcacggcggc
     5881 gagtgcgggg gttccaaggg ggcagcgcca ccttgggcaa ggccgaaggc cgcgcagtcg
     5941 atcaacaagc cccggagggg ccactttttg ccggaggggg agccgcgccg aaggcgtggg
     6001 ggaaccccgc aggggtgccc ttctttgggc accaaagaac tagatatagg gcgaaatgcg
     6061 aaagacttaa aaatcaacaa cttaaaaaag gggggtacgc aacagctcat tgcggcaccc
     6121 cccgcaatag ctcattgcgt aggttaaaga aaatctgtaa ttgactgcca cttttacgca
     6181 acgcataatt gttgtcgcgc tgccgaaaag ttgcagctga ttgcgcatgg tgccgcaacc
     6241 gtgcggcacc ctaccgcatg gagataagca tggccacgca gtccagagaa atcggcattc
     6301 aagccaagaa caagcccggt cactgggtgc aaacggaacg caaagcgcat gaggcgtggg
     6361 ccgggcttat tgcgaggaaa cccacggcgg caatgctgct gcatcacctc gtggcgcaga
     6421 tgggccacca gaacgccgtg gtggtcagcc agaagacact ttccaagctc atcggacgtt
     6481 ctttgcggac ggtccaatac gcagtcaagg acttggtggc cgagcgctgg atctccgtcg
     6541 tgaagctcaa cggccccggc accgtgtcgg cctacgtggt caatgaccgc gtggcgtggg
     6601 gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg gttgatcacg
     6661 acgaccagga cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg accctgtatc
     6721 cgggcgagca gcaactaccg accggccccg gcgaggagcc gcccagccag cccggcattc
     6781 cgggcatgga accagacctg ccagccttga ccgaaacgga ggaatgggaa cggcgcgggc
     6841 agcagcgcct gccgatgccc gatgagccgt gttttctgga cgatggcgag ccgttggagc
     6901 cgccgacacg ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca acccgtaagt
     6961 gcgctgttcc agactatcgg ctgtagccgc ctcgccgccc tataccttgt ctgcctcccc
     7021 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc
     7081 tcgctaacgg attcaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt
     7141 caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca
     7201 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
     7261 tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt
     7321 catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact
     7381 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tcggcctatt ggttaaaaaa
     7441 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
     7501 cattcgccat tcaggctgcg caactgttgg gaagg
//
