LOCUS       40924_47473        6546 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYanni2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Graupner S, Wackernagel W.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Graupner S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet 
            Oldenburg, Postfach 2503, 26111 Oldenburg, GERMANY
REFERENCE   3  (bases 1 to 6546)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6546)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet Oldenburg,
            Postfach 2503, 26111 Oldenburg, GERMANY"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1153)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1154..1231)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1465..1493
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1501..1517
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1770..1856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1948..1975
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1994..2085
                     /label=AmpR promoter
     CDS             2086..2943
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(3588..4118)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(4326..4354)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4511..5280
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             5281..5940
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cgaacgccag caagacgtag cccagcgcgt cgcttgcaat tcgcgctaac ttacattaat
       61 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      181 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      241 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttgacg
      301 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      361 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      421 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      481 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      541 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      601 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
      661 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
      721 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
      781 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
      841 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
      901 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
      961 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1021 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1081 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1141 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1201 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gccgcttcgc cttcgcgcgc
     1261 gaattgcaag ctgatccggc ggagcttatc gactgcacgg tgcaccaatg cttctggcgt
     1321 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
     1381 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     1441 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagaattcc cggggatccg tcgacctgca
     1561 gccaagcttc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1621 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     1681 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1741 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1861 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     1921 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     1981 ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagc tggccgccac
     3361 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     3421 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     3481 actcaatgct tgagtagggt accgagctcg aattggccgc ggcgttgtga caatttaccg
     3541 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     3601 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     3661 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     3721 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     3781 actgcgagat catagatata gatctcacta cgcggctgct caaacctggg cagaacgtaa
     3841 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     3901 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     3961 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     4021 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     4081 ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctgc gtaacatcgt
     4141 tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc
     4201 gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt
     4261 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc
     4321 attacagttt acgaaccgaa caggcttatg tcaattcgag ctcggtaccc actactcact
     4381 agactttgct tcgcaaagtc gtgaccgcct acggcggctg cggcgcccta cgggcttgct
     4441 ctccgggctt cgccctgcgc ggtcgctgcg ctcccttgcc agcccgtgga tatgtggacg
     4501 atggccgcga gcggccaccg gctggctcgc ttcgctcggc ccgtggacaa ccctgctgga
     4561 caagctgatg gacaggctgc gcctgcccac gagcttgacc acagggattg cccaccggct
     4621 acccagcctt cgaccacata cccaccggct ccaactgcgc ggcctgcggc cttgccccat
     4681 caattttttt aattttctct ggggaaaagc ctccggcctg cggcctgcgc gcttcgcttg
     4741 ccggttggac accaagtgga aggcgggtca aggctcgcgc agcgaccgcg cagcggcttg
     4801 gccttgacgc gcctggaacg acccaagcct atgcgagtgg gggcagtcga aggcgaagcc
     4861 cgcccgcctg ccccccgagc ctcacggcgg cgagtgcggg ggttccaagg gggcagcgcc
     4921 accttgggca aggccgaagg ccgcgcagtc gatcaacaag ccccggaggg gccacttttt
     4981 gccggagggg gagccgcgcc gaaggcgtgg gggaaccccg caggggtgcc cttctttggg
     5041 caccaaagaa ctagatatag ggcgaaatgc gaaagactta aaaatcaaca acttaaaaaa
     5101 ggggggtacg caacagctca ttgcggcacc ccccgcaata gctcattgcg taggttaaag
     5161 aaaatctgta attgactgcc acttttacgc aacgcataat tgttgtcgcg ctgccgaaaa
     5221 gttgcagctg attgcgcatg gtgccgcaac cgtgcggcac cctaccgcat ggagataagc
     5281 atggccacgc agtccagaga aatcggcatt caagccaaga acaagcccgg tcactgggtg
     5341 caaacggaac gcaaagcgca tgaggcgtgg gccgggctta ttgcgaggaa acccacggcg
     5401 gcaatgctgc tgcatcacct cgtggcgcag atgggccacc agaacgccgt ggtggtcagc
     5461 cagaagacac tttccaagct catcggacgt tctttgcgga cggtccaata cgcagtcaag
     5521 gacttggtgg ccgagcgctg gatctccgtc gtgaagctca acggccccgg caccgtgtcg
     5581 gcctacgtgg tcaatgaccg cgtggcgtgg ggccagcccc gcgaccagtt gcgcctgtcg
     5641 gtgttcagtg ccgccgtggt ggttgatcac gacgaccagg acgaatcgct gttggggcat
     5701 ggcgacctgc gccgcatccc gaccctgtat ccgggcgagc agcaactacc gaccggcccc
     5761 ggcgaggagc cgcccagcca gcccggcatt ccgggcatgg aaccagacct gccagccttg
     5821 accgaaacgg aggaatggga acggcgcggg cagcagcgcc tgccgatgcc cgatgagccg
     5881 tgttttctgg acgatggcga gccgttggag ccgccgacac gggtcacgct gccgcgccgg
     5941 tagcacttgg gttgcgcagc aacccgtaag tgcgctgttc cagactatcg gctgtagccg
     6001 cctcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     6061 cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccgt ttttatcagg
     6121 ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga
     6181 gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa
     6241 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
     6301 gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     6361 agcaaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc
     6421 actcatcgca gtcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6481 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
     6541 ggaagg
//
