LOCUS       40924_47468        5670 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYanni1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Graupner S, Wackernagel W.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Graupner S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet 
            Oldenburg, Postfach 2503, 26111 Oldenburg, GERMANY
REFERENCE   3  (bases 1 to 5670)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JUN-1999) Graupner S., FB7 / AG Genetik, Universitaet Oldenburg,
            Postfach 2503, 26111 Oldenburg, GERMANY"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..1153)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(1154..1231)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        1465..1493
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    1501..1517
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    1533..1569
                     /label=multiple cloning site
                     /note="multiple cloning site"
     rRNA            1649..1768
                     /gene="5S rRNA"
                     /product="5S ribosomal RNA"
                     /label=5S rRNA
     gene            1649..1768
                     /gene="5S rRNA"
                     /label=5S rRNA
     terminator      1770..1856
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1948..1975
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        1994..2085
                     /label=AmpR promoter
     CDS             2086..2943
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3635..4404
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             4405..5064
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cgaacgccag caagacgtag cccagcgcgt cgcttgcaat tcgcgctaac ttacattaat
       61 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      121 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
      181 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
      241 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttgacg
      301 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
      361 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      421 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      481 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      541 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      601 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
      661 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
      721 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
      781 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
      841 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
      901 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
      961 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     1021 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     1081 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     1141 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     1201 cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gccgcttcgc cttcgcgcgc
     1261 gaattgcaag ctgatccggc ggagcttatc gactgcacgg tgcaccaatg cttctggcgt
     1321 caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg cataattcgt
     1381 gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc ataacggttc
     1441 tggcaaatat tctgaaatga gctgttgaca attaatcatc ggctcgtata atgtgtggaa
     1501 ttgtgagcgg ataacaattt cacacaggaa acagaattcc cggggatccg tcgacctgca
     1561 gccaagcttc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     1621 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     1681 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     1741 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1861 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     1921 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     1981 ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     2101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     2161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     2221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     2281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     2341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     2401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     2461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     2521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     2581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     3001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     3061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     3301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagc tggccgccac
     3361 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
     3421 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
     3481 actcaatgct tgagtatact cactagactt tgcttcgcaa agtcgtgacc gcctacggcg
     3541 gctgcggcgc cctacgggct tgctctccgg gcttcgccct gcgcggtcgc tgcgctccct
     3601 tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc tcgcttcgct
     3661 cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc ccacgagctt
     3721 gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc ggctccaact
     3781 gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa aagcctccgg
     3841 cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg gtcaaggctc
     3901 gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa gcctatgcga
     3961 gtgggggcag tcgaaggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg
     4021 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa
     4081 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac
     4141 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga
     4201 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc
     4261 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca
     4321 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg
     4381 gcaccctacc gcatggagat aagcatggcc acgcagtcca gagaaatcgg cattcaagcc
     4441 aagaacaagc ccggtcactg ggtgcaaacg gaacgcaaag cgcatgaggc gtgggccggg
     4501 cttattgcga ggaaacccac ggcggcaatg ctgctgcatc acctcgtggc gcagatgggc
     4561 caccagaacg ccgtggtggt cagccagaag acactttcca agctcatcgg acgttctttg
     4621 cggacggtcc aatacgcagt caaggacttg gtggccgagc gctggatctc cgtcgtgaag
     4681 ctcaacggcc ccggcaccgt gtcggcctac gtggtcaatg accgcgtggc gtggggccag
     4741 ccccgcgacc agttgcgcct gtcggtgttc agtgccgccg tggtggttga tcacgacgac
     4801 caggacgaat cgctgttggg gcatggcgac ctgcgccgca tcccgaccct gtatccgggc
     4861 gagcagcaac taccgaccgg ccccggcgag gagccgccca gccagcccgg cattccgggc
     4921 atggaaccag acctgccagc cttgaccgaa acggaggaat gggaacggcg cgggcagcag
     4981 cgcctgccga tgcccgatga gccgtgtttt ctggacgatg gcgagccgtt ggagccgccg
     5041 acacgggtca cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg taagtgcgct
     5101 gttccagact atcggctgta gccgcctcgc cgccctatac cttgtctgcc tccccgcgtt
     5161 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
     5221 aacggattca ccgtttttat caggctctgg gaggcagaat aaatgatcat atcgtcaatt
     5281 attacctcca cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc
     5341 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
     5401 cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc
     5461 gcttattatc acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt
     5521 aaaaaaatta cgccccgccc tgccactcat cgcagtcggc ctattggtta aaaaatgagc
     5581 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc
     5641 gccattcagg ctgcgcaact gttgggaagg
//
