LOCUS       40924_48318        9208 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYZ81N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Zhao Y, Elder RT, Chen M, Cao J.
  TITLE     Fission yeast expression vectors adapted for positive identification
            of gene insertion and green fluorescent protein fusion
  JOURNAL   BioTechniques 25 (3), 438-440 (1998)
  PUBMED    9762442
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Zhao Y, Chen M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1998) Departments of Pediatrics and 
            Microbiology-Immunology, Northwestern University Medical School, 
            2430 N Halsted Street, #218, Chicago, IL 60614, USA
REFERENCE   3  (bases 1 to 9208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "25"; issue: "3"; pages: 
            "438-440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1998) Departments of Pediatrics and Microbiology-Immunology,
            Northwestern University Medical School, 2430 N Halsted Street, #218,
            Chicago, IL 60614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1205
                     /label=nmt1 P41 promoter
                     /note="mutant nmt1 promoter from Schizosaccharomyces pombe,
                     conferring medium strength thiamine-repressible expression"
     primer_bind     1350..1366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1367..1423
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1436..1452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1460..1476)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1484..1514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1529..1550)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      1649..2656
                     /label=from Schizosaccharomyces pombe nmt1
                     /note="from Schizosaccharomyces pombe nmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(2729..3512)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(3862..3878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4091..4546
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4828..4932
                     /label=AmpR promoter
     CDS             4933..5790
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5964..6552
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6840..6861
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6876..6906
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6914..6930
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6938..6954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7458..8549)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(8562..8966)
                     /label=LEU2 promoter
ORIGIN
        1 agcttgcatg cctgcaggtc gatcgactct agaggatcag aaaattatcg ccataaaaga
       61 cagaataagt catcagcggt tgtttcattt cctatatttt ttttttattt ttttattttt
      121 taataaggga aaatttaacg tctaaggata cagaagattg ttagcacatt aaagtaataa
      181 aggcttaagt agtaagtgcc ttagcatgtt attgtatttc aaaggacata atctaaaata
      241 ataacaatat catttctcac aagttattca attttctttt ttttttctaa taatatcaag
      301 aatgtattat ttgtttgaca taagtcaact aatttattta atatgctgga ttaatcttgc
      361 agacatgtaa attaacaagt tttagtcaaa taacgttgaa gtttcaatga actcaaataa
      421 tttctctttt tttttatata acnnnnnntc taatctgatt tatattttcc gcagggatca
      481 actgaagtta tgacatttgg attggatcac ttataacctt ggtcgccaaa taatacaaaa
      541 atcagcgtta taaaacaaag aaggtttttg ttaagaaatt aatcctcttt cttgataaga
      601 aagttgaacc gaaattgcag atactgatat atgaaaataa tacccacaat tttgggaata
      661 gcgcaagcct caatttaaac aataggtgag gacacatgat aatgacctca atgattgtta
      721 gaagaaaaga gcctcattac aaaatcgaaa aatgaatggt tgggtacaag tttccaaaac
      781 atggtaaagt ggactttgcg tatgagacgt aaatagaaaa aaacacttgt tatatgtttt
      841 ctagaattat tgttgtctct ttatggttgg atgatgcaaa atagtaattt cggttagttg
      901 ctgtaaaaca ccacgagaca aatagatatg gatatttatt aaatcaggaa aaacgtaact
      961 ctcggctact ggatggttca gtcacccaac gattactggg gagagaaaac agggcaaaag
     1021 caaagcttaa aggaatccga ttgtcattcg gcaatgtgca gcgaaactaa aaaccggata
     1081 atggacctgt taatcgaaac attgaagatg gaagaggaat cctggcatat catcaattga
     1141 ataagttgaa ttaattattt caatctcatt ctcactttct gacttatagt cgctttgtta
     1201 aatcatatgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     1261 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     1321 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg
     1381 tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat
     1441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1621 aacgcgcggg gagaggcggt ttgcgtattg cggccgctaa aaggaatgtc tcccttgcca
     1681 gtactgctag ggtttttctt tcaaactatg gaagcccatt caagctgcat attacgattt
     1741 tgtttttcgc ttttagaaag tggtttagat gagataatag aaaaattctt gatctccgac
     1801 aacgagtact tttatttttt ttgctaatca ctttactcaa tattagctcg aaatcgtaga
     1861 aacgtagacg ggtgcgggat accgagtggt gtagttaaga atttttataa accacgtggc
     1921 ccaaaaatat gaacccaaaa cgtttataca tgagtatact ttaagaaggc tatacccctt
     1981 cgtgttagat gtagttttag ctacccaacc cgagtctatg agcttgactt cagatgtaga
     2041 aggcattaaa tcgttttgaa tattaattaa aaaacgatga aaattaaata tttaaaagca
     2101 atcatacgct gaaaatttag tgctgtggct aatccttcaa catggaaatg ccataaaagt
     2161 gactttgaca aaaaaaaaag tatatacagg tagtaaactc atctacttca ttgactttgt
     2221 ttacagcatg tggaaggagg aatatttatt gctaaatcgt agtttaacat tcaataagta
     2281 atactattga aattcgacaa gattggccgc atggatgaaa aagaggcatt ttgctttggg
     2341 agaattagtt caaattagaa ctgaaaaaaa aaactttacg aggcaaaaat gtcggattga
     2401 gatcgtaaaa gttcgctcgt cgtcttttgc tttgtgattg ttttcatgga tacatcttgc
     2461 tggatattta aattttagta ctatgtataa gatattctat aaatgtttta tcacccaaac
     2521 ctgttagcgc cttcttaatt ctattcaatc tggcttttgc tctgagacta cttcttggac
     2581 tttcactact tgttagttat acggaatttg tgtaattaga agtgaaataa tcctttctat
     2641 tagtaatgcg agctcgaatt cgagtctaac tccttaacca ctcggacata gtgacttatc
     2701 tgacactcat tgtaaattaa tatatatata ggcattttgt ttagttaaag gtacttaagt
     2761 aattagtata aacgaaccaa ttttataatc aggaagttaa gtgaatggta gcacatgtcg
     2821 taaaaattgt gaatttttat tgaataatat tttaaataca agcctttcta gactaggtat
     2881 actcataaac atatatgagc aaaaggatag aggagattac attgcatctt ctacaaattt
     2941 atttattgcc ctttactgaa aaattaaata atgagtacta aatgataaaa agcgctcagt
     3001 acaagaaaga ttgcaaaaat attgcattct tcatgaatta aagttgcata taaggcatat
     3061 tgaaagtaat agtactaaaa cagcagttag cgaaaattaa tagaattata ttcgcaagac
     3121 aattgtgaca ttaaattaaa aaattgtaga atttttacta tcctctttaa cgccatgagc
     3181 ctttataaaa aggttaaatt agttttaaca ttcttttttt gagtaagagt ttacaattta
     3241 tcaaaacctg tgttattata ttcattagtt tcaatttatt agcatctaga gaaaaatcaa
     3301 ttggcagtta cattgttgga atttatgaag aaaaagaatc tacaacggag aataagttgc
     3361 tgatcgcttt ccctaaaatt gtatattttg ctgagcttat tttgacattt cgttgaagtt
     3421 ttctctaatt cgcattcatt ttaagtaaaa caatgagaaa taaaattaca aaaaatacaa
     3481 attaaaatac aatttttagc tataattata gacgatgccc ttgtatccca ttctgtctcg
     3541 cttgccccta ctttttatct tttatatacc ataatgaacg ctgccgctac taaccatacc
     3601 ccgattttac atttcggact cccaaggacg tacaaaatag aaaactatag aaaaaaataa
     3661 tcagaaaata gcatgtcatc tctttgtaaa acgcgtttgc aagaagaaag gaaacaatgg
     3721 agaagagatc atccatttgt atgtaaattt tagtaaactt gaagaaatca ctaacaactt
     3781 ctcttactta gggattctat gcaaaacctt gtaaatcatc tgatggagga ctcgatttaa
     3841 tgaattggaa ggttggaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3901 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3961 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     4021 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
     4081 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4141 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
     4201 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4261 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
     4321 cgtagtggga catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4381 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
     4441 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
     4501 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
     4561 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     4621 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     4681 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     4741 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     4801 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4861 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4921 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4981 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5041 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5101 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5161 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5221 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5281 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5341 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5401 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5461 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5521 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5581 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5641 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5701 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5761 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     6721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     6781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     6841 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     6901 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     6961 tacgccaagc ttgtcgatcg actacgtcgt taaggccgtt tctgacagag taaaattctt
     7021 gagggaactt tcaccattat gggaaatggt tcaagaaggt attgacttaa actccatcaa
     7081 atggtcaggt cattgagtgt tttttatttg ttgtattttt ttttttttag agaaaatcct
     7141 ccaatatcaa attaggaatc gtagtttcat gattttctgt tacacctaac tttttgtgtg
     7201 gtgccctcct ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga
     7261 gccattagta tcaatttgct tacctgtatt cctttactat cctccttttt ctccttcttg
     7321 ataaatgtat gtagattgcg tatatagttt cgtctaccct atgaacatat tccattttgt
     7381 aatttcgtgt cgtttctatt atgaatttca tttataaagt ttatgtacaa atatcataaa
     7441 aaaagagaat ctttttaagc aaggattttc ttaacttctt cggcgacagc atcaccgact
     7501 tcggtggtac tgttggaacc acctaaatca ccagttctga tacctgcatc caaaaccttt
     7561 ttaactgcat cttcaatggc cttaccttct tcaggcaagt tcaatgacaa tttcaacatc
     7621 attgcagcag acaagatagt ggcgataggg tcaaccttat tctttggcaa atctggagca
     7681 gaaccgtggc atggttcgta caaaccaaat gcggtgttct tgtctggcaa agaggccaag
     7741 gacgcagatg gcaacaaacc caaggaacct gggataacgg aggcttcatc ggagatgata
     7801 tcaccaaaca tgttgctggt gattataata ccatttaggt gggttgggtt cttaactagg
     7861 atcatggcgg cagaatcaat caattgatgt tgaaccttca atgtagggaa ttcgttcttg
     7921 atggtttcct ccacagtttt tctccataat cttgaagagg ccaaaacatt agctttatcc
     7981 aaggaccaaa taggcaatgg tggctcatgt tgtagggcca tgaaagcggc cattcttgtg
     8041 attctttgca cttctggaac ggtgtattgt tcactatccc aagcgacacc atcaccatcg
     8101 tcttcctttc tcttaccaaa gtaaatacct cccactaatt ctctgacaac aacgaagtca
     8161 gtacctttag caaattgtgg cttgattgga gataagtcta aaagagagtc ggatgcaaag
     8221 ttacatggtc ttaagttggc gtacaattga agttctttac ggatttttag taaaccttgt
     8281 tcaggtctaa cactaccggt accccattta ggaccaccca cagcacctaa caaaacggca
     8341 tcaaccttct tggaggcttc cagcgcctca tctggaagtg ggacacctgt agcatcgata
     8401 gcagcaccac caattaaatg attttcgaaa tcgaacttga cattggaacg aacatcagaa
     8461 atagctttaa gaaccttaat ggcttcggct gtgatttctt gaccaacgtg gtcacctggc
     8521 aaaacgacga tcttcttagg ggcagacata ggggcagaca ttagaatggt atatccttga
     8581 aatatatata tatattgctg aaatgtaaaa ggtaagaaaa gttagaaagt aagacgattg
     8641 ctaaccacct attggaaaaa acaataggtc cttaaataat attgtcaact tcaagtattg
     8701 tgatgcaagc atttagtcat gaacgcttct ctattctata tgaaaagccg gttccggcct
     8761 ctcacctttc ctttttctcc caatttttca gttgaaaaag gtatatgcgt caggcgacct
     8821 ctgaaattaa caaaaaattt ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg
     8881 tgttctcgtt atgttgagga aaaaaataat ggttgctaag agattcgaac tcttgcatct
     8941 tacgatacct gagtattccc acagttaact gcggtcaaga tatttcttga atcaggcgcc
     9001 ttagaccgct cggccaaaca accaattact tgttgagaaa tagagtataa ttatcctata
     9061 aatataacgt ttttgaacac acatgaacaa ggaagtacag gacaattgat tttgaagaga
     9121 atgtggattt tgatgtaatt gttgggattc catttttaat aaggcaataa tattaggtat
     9181 gtagatatac tagaagttct cctcgaca
//
