LOCUS       40924_48313        9211 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYZ41N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Zhao Y, Elder RT, Chen M, Cao J.
  TITLE     Fission yeast expression vectors adapted for positive identification
            of gene insertion and green fluorescent protein fusion
  JOURNAL   BioTechniques 25 (3), 438-440 (1998)
  PUBMED    9762442
REFERENCE   2  (bases 1 to 9211)
  AUTHORS   Zhao Y, Chen M.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-1998) Departments of Pediatrics and 
            Microbiology-Immunology, Northwestern University Medical School, 
            2430 N Halsted Street, #218, Chicago, IL 60614, USA
REFERENCE   3  (bases 1 to 9211)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9211)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "25"; issue: "3"; pages: 
            "438-440"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-1998) Departments of Pediatrics and Microbiology-Immunology,
            Northwestern University Medical School, 2430 N Halsted Street, #218,
            Chicago, IL 60614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1208
                     /label=nmt1 promoter
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     primer_bind     1353..1369
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1370..1426
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1439..1455)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1463..1479)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1487..1517)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1532..1553)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      1652..2659
                     /label=from Schizosaccharomyces pombe nmt1
                     /note="from Schizosaccharomyces pombe nmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(2732..3515)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(3865..3881)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4094..4549
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4831..4935
                     /label=AmpR promoter
     CDS             4936..5793
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5967..6555
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6843..6864
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6879..6909
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6917..6933
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6941..6957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7461..8552)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(8565..8969)
                     /label=LEU2 promoter
ORIGIN
        1 agcttgcatg cctgcaggtc gatcgactct agaggatcag aaaattatcg ccataaaaga
       61 cagaataagt catcagcggt tgtttcattt cctatatttt ttttttattt ttttattttt
      121 taataaggga aaatttaacg tctaaggata cagaagattg ttagcacatt aaagtaataa
      181 aggcttaagt agtaagtgcc ttagcatgtt attgtatttc aaaggacata atctaaaata
      241 ataacaatat catttctcac aagttattca attttctttt ttttttctaa taatatcaag
      301 aatgtattat ttgtttgaca taagtcaact aatttattta atatgctgga ttaatcttgc
      361 agacatgtaa attaacaagt tttagtcaaa taacgttgaa gtttcaatga actcaaataa
      421 tttctctttt tttttatata acnnnnnntc taatctgatt tatattttcc gcagggatca
      481 actgaagtta tgacatttgg attggatcac ttataacctt ggtcgccaaa taatacaaaa
      541 atcagcgtta taaaacaaag aaggtttttg ttaagaaatt aatcctcttt cttgataaga
      601 aagttgaacc gaaattgcag atactgatat atgaaaataa tacccacaat tttgggaata
      661 gcgcaagcct caatttaaac aataggtgag gacacatgat aatgacctca atgattgtta
      721 gaagaaaaga gcctcattac aaaatcgaaa aatgaatggt tgggtacaag tttccaaaac
      781 atggtaaagt ggactttgcg tatgagacgt aaatagaaaa aaacacttgt tatatgtttt
      841 ctagaattat tgttgtctct ttatggttgg atgatgcaaa atagtaattt cggttagttg
      901 ctgtaaaaca ccacgagaca aatagatatg gatatttatt aaatcaggaa aaacgtaact
      961 ctcggctact ggatggttca gtcacccaac gattactggg gagagaaaac agggcaaaag
     1021 caaagcttaa aggaatccga ttgtcattcg gcaatgtgca gcgaaactaa aaaccggata
     1081 atggacctgt taatcgaaac attgaagata aaggaagagg aatcctggca tatcatcaat
     1141 tgaataagtt gaattaatta tttcaatctc attctcactt tctgacttat agtcgctttg
     1201 ttaaatcata tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1261 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1321 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct
     1381 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt
     1441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1501 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1561 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1621 gccaacgcgc ggggagaggc ggtttgcgta ttgcggccgc taaaaggaat gtctcccttg
     1681 ccagtactgc tagggttttt ctttcaaact atggaagccc attcaagctg catattacga
     1741 ttttgttttt cgcttttaga aagtggttta gatgagataa tagaaaaatt cttgatctcc
     1801 gacaacgagt acttttattt tttttgctaa tcactttact caatattagc tcgaaatcgt
     1861 agaaacgtag acgggtgcgg gataccgagt ggtgtagtta agaattttta taaaccacgt
     1921 ggcccaaaaa tatgaaccca aaacgtttat acatgagtat actttaagaa ggctataccc
     1981 cttcgtgtta gatgtagttt tagctaccca acccgagtct atgagcttga cttcagatgt
     2041 agaaggcatt aaatcgtttt gaatattaat taaaaaacga tgaaaattaa atatttaaaa
     2101 gcaatcatac gctgaaaatt tagtgctgtg gctaatcctt caacatggaa atgccataaa
     2161 agtgactttg acaaaaaaaa aagtatatac aggtagtaaa ctcatctact tcattgactt
     2221 tgtttacagc atgtggaagg aggaatattt attgctaaat cgtagtttaa cattcaataa
     2281 gtaatactat tgaaattcga caagattggc cgcatggatg aaaaagaggc attttgcttt
     2341 gggagaatta gttcaaatta gaactgaaaa aaaaaacttt acgaggcaaa aatgtcggat
     2401 tgagatcgta aaagttcgct cgtcgtcttt tgctttgtga ttgttttcat ggatacatct
     2461 tgctggatat ttaaatttta gtactatgta taagatattc tataaatgtt ttatcaccca
     2521 aacctgttag cgccttctta attctattca atctggcttt tgctctgaga ctacttcttg
     2581 gactttcact acttgttagt tatacggaat ttgtgtaatt agaagtgaaa taatcctttc
     2641 tattagtaat gcgagctcga attcgagtct aactccttaa ccactcggac atagtgactt
     2701 atctgacact cattgtaaat taatatatat ataggcattt tgtttagtta aaggtactta
     2761 agtaattagt ataaacgaac caattttata atcaggaagt taagtgaatg gtagcacatg
     2821 tcgtaaaaat tgtgaatttt tattgaataa tattttaaat acaagccttt ctagactagg
     2881 tatactcata aacatatatg agcaaaagga tagaggagat tacattgcat cttctacaaa
     2941 tttatttatt gccctttact gaaaaattaa ataatgagta ctaaatgata aaaagcgctc
     3001 agtacaagaa agattgcaaa aatattgcat tcttcatgaa ttaaagttgc atataaggca
     3061 tattgaaagt aatagtacta aaacagcagt tagcgaaaat taatagaatt atattcgcaa
     3121 gacaattgtg acattaaatt aaaaaattgt agaattttta ctatcctctt taacgccatg
     3181 agcctttata aaaaggttaa attagtttta acattctttt tttgagtaag agtttacaat
     3241 ttatcaaaac ctgtgttatt atattcatta gtttcaattt attagcatct agagaaaaat
     3301 caattggcag ttacattgtt ggaatttatg aagaaaaaga atctacaacg gagaataagt
     3361 tgctgatcgc tttccctaaa attgtatatt ttgctgagct tattttgaca tttcgttgaa
     3421 gttttctcta attcgcattc attttaagta aaacaatgag aaataaaatt acaaaaaata
     3481 caaattaaaa tacaattttt agctataatt atagacgatg cccttgtatc ccattctgtc
     3541 tcgcttgccc ctacttttta tcttttatat accataatga acgctgccgc tactaaccat
     3601 accccgattt tacatttcgg actcccaagg acgtacaaaa tagaaaacta tagaaaaaaa
     3661 taatcagaaa atagcatgtc atctctttgt aaaacgcgtt tgcaagaaga aaggaaacaa
     3721 tggagaagag atcatccatt tgtatgtaaa ttttagtaaa cttgaagaaa tcactaacaa
     3781 cttctcttac ttagggattc tatgcaaaac cttgtaaatc atctgatgga ggactcgatt
     3841 taatgaattg gaaggttgga attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3901 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4021 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     4081 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4141 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     4201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     4321 tcacgtagtg ggacatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     4441 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     4501 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     4561 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4621 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4681 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4741 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4801 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4861 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4921 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4981 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5041 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5101 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5161 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5221 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5281 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5341 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5401 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5461 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5521 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5581 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5641 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5701 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5761 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5821 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5881 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5941 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6001 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6061 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     6121 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6181 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6241 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6301 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6361 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6421 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6481 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6541 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6601 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6661 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6721 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6781 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6841 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6901 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6961 gattacgcca agcttgtcga tcgactacgt cgttaaggcc gtttctgaca gagtaaaatt
     7021 cttgagggaa ctttcaccat tatgggaaat ggttcaagaa ggtattgact taaactccat
     7081 caaatggtca ggtcattgag tgttttttat ttgttgtatt tttttttttt tagagaaaat
     7141 cctccaatat caaattagga atcgtagttt catgattttc tgttacacct aactttttgt
     7201 gtggtgccct cctccttgtc aatattaatg ttaaagtgca attctttttc cttatcacgt
     7261 tgagccatta gtatcaattt gcttacctgt attcctttac tatcctcctt tttctccttc
     7321 ttgataaatg tatgtagatt gcgtatatag tttcgtctac cctatgaaca tattccattt
     7381 tgtaatttcg tgtcgtttct attatgaatt tcatttataa agtttatgta caaatatcat
     7441 aaaaaaagag aatcttttta agcaaggatt ttcttaactt cttcggcgac agcatcaccg
     7501 acttcggtgg tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc
     7561 tttttaactg catcttcaat ggccttacct tcttcaggca agttcaatga caatttcaac
     7621 atcattgcag cagacaagat agtggcgata gggtcaacct tattctttgg caaatctgga
     7681 gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc
     7741 aaggacgcag atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg
     7801 atatcaccaa acatgttgct ggtgattata ataccattta ggtgggttgg gttcttaact
     7861 aggatcatgg cggcagaatc aatcaattga tgttgaacct tcaatgtagg gaattcgttc
     7921 ttgatggttt cctccacagt ttttctccat aatcttgaag aggccaaaac attagcttta
     7981 tccaaggacc aaataggcaa tggtggctca tgttgtaggg ccatgaaagc ggccattctt
     8041 gtgattcttt gcacttctgg aacggtgtat tgttcactat cccaagcgac accatcacca
     8101 tcgtcttcct ttctcttacc aaagtaaata cctcccacta attctctgac aacaacgaag
     8161 tcagtacctt tagcaaattg tggcttgatt ggagataagt ctaaaagaga gtcggatgca
     8221 aagttacatg gtcttaagtt ggcgtacaat tgaagttctt tacggatttt tagtaaacct
     8281 tgttcaggtc taacactacc ggtaccccat ttaggaccac ccacagcacc taacaaaacg
     8341 gcatcaacct tcttggaggc ttccagcgcc tcatctggaa gtgggacacc tgtagcatcg
     8401 atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga acgaacatca
     8461 gaaatagctt taagaacctt aatggcttcg gctgtgattt cttgaccaac gtggtcacct
     8521 ggcaaaacga cgatcttctt aggggcagac ataggggcag acattagaat ggtatatcct
     8581 tgaaatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
     8641 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
     8701 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
     8761 cctctcacct ttcctttttc tcccaatttt tcagttgaaa aaggtatatg cgtcaggcga
     8821 cctctgaaat taacaaaaaa tttccagtca tcgaatttga ttctgtgcga tagcgcccct
     8881 gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg aactcttgca
     8941 tcttacgata cctgagtatt cccacagtta actgcggtca agatatttct tgaatcaggc
     9001 gccttagacc gctcggccaa acaaccaatt acttgttgag aaatagagta taattatcct
     9061 ataaatataa cgtttttgaa cacacatgaa caaggaagta caggacaatt gattttgaag
     9121 agaatgtgga ttttgatgta attgttggga ttccattttt aataaggcaa taatattagg
     9181 tatgtagata tactagaagt tctcctcgac a
//
