LOCUS       40924_48303        9215 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYZ1N, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Zhao Y, Chen M, Elder RT, Cao J.
  TITLE     Fission yeast expression vectors adapted for large scale random 
            cloning and GFP fusion with positive selection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Zhao Y, Chen M, Elder RT, Cao J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-1998) Departments of Pediatrics and 
            Microbiology-Immunology, Northwestern University Medical School, 
            2430 N Halsted Street, #218, Chicago, IL 60614, USA
REFERENCE   3  (bases 1 to 9215)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-FEB-1998) Departments of Pediatrics and Microbiology-Immunology,
            Northwestern University Medical School, 2430 N Halsted Street, #218,
            Chicago, IL 60614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1212
                     /label=nmt1 promoter
                     /note="wild-type nmt1 promoter from Schizosaccharomyces
                     pombe, conferring strong thiamine-repressible expression"
     primer_bind     1357..1373
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1374..1430
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1443..1459)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1467..1483)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1491..1521)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1536..1557)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     regulatory      1656..2663
                     /label=from nmt1
                     /note="from nmt1"
                     /regulatory_class="terminator"
     rep_origin      complement(2736..3519)
                     /direction=LEFT
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(3869..3885)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4098..4553
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4835..4939
                     /label=AmpR promoter
     CDS             4940..5797
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5971..6559
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6847..6868
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6883..6913
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6921..6937
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6945..6961
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7465..8556)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(8569..8973)
                     /label=LEU2 promoter
ORIGIN
        1 agcttgcatg cctgcaggtc gatcgactct agaggatcag aaaattatcg ccataaaaga
       61 cagaataagt catcagcggt tgtttcattt cctatatttt ttttttattt ttttattttt
      121 taataaggga aaatttaacg tctaaggata cagaagattg ttagcacatt aaagtaataa
      181 aggcttaagt agtaagtgcc ttagcatgtt attgtatttc aaaggacata atctaaaata
      241 ataacaatat catttctcac aagttattca attttctttt ttttttctaa taatatcaag
      301 aatgtattat ttgtttgaca taagtcaact aatttattta atatgctgga ttaatcttgc
      361 agacatgtaa attaacaagt tttagtcaaa taacgttgaa gtttcaatga actcaaataa
      421 tttctctttt tttttatata acnnnnnntc taatctgatt tatattttcc gcagggatca
      481 actgaagtta tgacatttgg attggatcac ttataacctt ggtcgccaaa taatacaaaa
      541 atcagcgtta taaaacaaag aaggtttttg ttaagaaatt aatcctcttt cttgataaga
      601 aagttgaacc gaaattgcag atactgatat atgaaaataa tacccacaat tttgggaata
      661 gcgcaagcct caatttaaac aataggtgag gacacatgat aatgacctca atgattgtta
      721 gaagaaaaga gcctcattac aaaatcgaaa aatgaatggt tgggtacaag tttccaaaac
      781 atggtaaagt ggactttgcg tatgagacgt aaatagaaaa aaacacttgt tatatgtttt
      841 ctagaattat tgttgtctct ttatggttgg atgatgcaaa atagtaattt cggttagttg
      901 ctgtaaaaca ccacgagaca aatagatatg gatatttatt aaatcaggaa aaacgtaact
      961 ctcggctact ggatggttca gtcacccaac gattactggg gagagaaaac agggcaaaag
     1021 caaagcttaa aggaatccga ttgtcattcg gcaatgtgca gcgaaactaa aaaccggata
     1081 atggacctgt taatcgaaac attgaagata tataaaggaa gaggaatcct ggcatatcat
     1141 caattgaata agttgaatta attatttcaa tctcattctc actttctgac ttatagtcgc
     1201 tttgttaaat catatgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     1261 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     1321 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     1381 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca
     1441 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1501 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1561 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1621 atcggccaac gcgcggggag aggcggtttg cgtattgcgg ccgctaaaag gaatgtctcc
     1681 cttgccagta ctgctagggt ttttctttca aactatggaa gcccattcaa gctgcatatt
     1741 acgattttgt ttttcgcttt tagaaagtgg tttagatgag ataatagaaa aattcttgat
     1801 ctccgacaac gagtactttt attttttttg ctaatcactt tactcaatat tagctcgaaa
     1861 tcgtagaaac gtagacgggt gcgggatacc gagtggtgta gttaagaatt tttataaacc
     1921 acgtggccca aaaatatgaa cccaaaacgt ttatacatga gtatacttta agaaggctat
     1981 accccttcgt gttagatgta gttttagcta cccaacccga gtctatgagc ttgacttcag
     2041 atgtagaagg cattaaatcg ttttgaatat taattaaaaa acgatgaaaa ttaaatattt
     2101 aaaagcaatc atacgctgaa aatttagtgc tgtggctaat ccttcaacat ggaaatgcca
     2161 taaaagtgac tttgacaaaa aaaaaagtat atacaggtag taaactcatc tacttcattg
     2221 actttgttta cagcatgtgg aaggaggaat atttattgct aaatcgtagt ttaacattca
     2281 ataagtaata ctattgaaat tcgacaagat tggccgcatg gatgaaaaag aggcattttg
     2341 ctttgggaga attagttcaa attagaactg aaaaaaaaaa ctttacgagg caaaaatgtc
     2401 ggattgagat cgtaaaagtt cgctcgtcgt cttttgcttt gtgattgttt tcatggatac
     2461 atcttgctgg atatttaaat tttagtacta tgtataagat attctataaa tgttttatca
     2521 cccaaacctg ttagcgcctt cttaattcta ttcaatctgg cttttgctct gagactactt
     2581 cttggacttt cactacttgt tagttatacg gaatttgtgt aattagaagt gaaataatcc
     2641 tttctattag taatgcgagc tcgaattcga gtctaactcc ttaaccactc ggacatagtg
     2701 acttatctga cactcattgt aaattaatat atatataggc attttgttta gttaaaggta
     2761 cttaagtaat tagtataaac gaaccaattt tataatcagg aagttaagtg aatggtagca
     2821 catgtcgtaa aaattgtgaa tttttattga ataatatttt aaatacaagc ctttctagac
     2881 taggtatact cataaacata tatgagcaaa aggatagagg agattacatt gcatcttcta
     2941 caaatttatt tattgccctt tactgaaaaa ttaaataatg agtactaaat gataaaaagc
     3001 gctcagtaca agaaagattg caaaaatatt gcattcttca tgaattaaag ttgcatataa
     3061 ggcatattga aagtaatagt actaaaacag cagttagcga aaattaatag aattatattc
     3121 gcaagacaat tgtgacatta aattaaaaaa ttgtagaatt tttactatcc tctttaacgc
     3181 catgagcctt tataaaaagg ttaaattagt tttaacattc tttttttgag taagagttta
     3241 caatttatca aaacctgtgt tattatattc attagtttca atttattagc atctagagaa
     3301 aaatcaattg gcagttacat tgttggaatt tatgaagaaa aagaatctac aacggagaat
     3361 aagttgctga tcgctttccc taaaattgta tattttgctg agcttatttt gacatttcgt
     3421 tgaagttttc tctaattcgc attcatttta agtaaaacaa tgagaaataa aattacaaaa
     3481 aatacaaatt aaaatacaat ttttagctat aattatagac gatgcccttg tatcccattc
     3541 tgtctcgctt gcccctactt tttatctttt atataccata atgaacgctg ccgctactaa
     3601 ccataccccg attttacatt tcggactccc aaggacgtac aaaatagaaa actatagaaa
     3661 aaaataatca gaaaatagca tgtcatctct ttgtaaaacg cgtttgcaag aagaaaggaa
     3721 acaatggaga agagatcatc catttgtatg taaattttag taaacttgaa gaaatcacta
     3781 acaacttctc ttacttaggg attctatgca aaaccttgta aatcatctga tggaggactc
     3841 gatttaatga attggaaggt tggaattcac tggccgtcgt tttacaacgt cgtgactggg
     3901 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     3961 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4021 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4081 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4141 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4201 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4261 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4321 tggttcacgt agtgggacat cgccctgata gacggttttt cgccctttga cgttggagtc
     4381 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     4441 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     4501 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     4561 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     4621 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     4681 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     4741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4801 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5161 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     6121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6601 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6661 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6721 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6781 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6841 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6901 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6961 ccatgattac gccaagcttg tcgatcgact acgtcgttaa ggccgtttct gacagagtaa
     7021 aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt gacttaaact
     7081 ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt tttttagaga
     7141 aaatcctcca atatcaaatt aggaatcgta gtttcatgat tttctgttac acctaacttt
     7201 ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc
     7261 acgttgagcc attagtatca atttgcttac ctgtattcct ttactatcct cctttttctc
     7321 cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg aacatattcc
     7381 attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata
     7441 tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc
     7501 accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa
     7561 aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt
     7621 caacatcatt gcagcagaca agatagtggc gatagggtca accttattct ttggcaaatc
     7681 tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga
     7741 ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga
     7801 gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt
     7861 aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg tagggaattc
     7921 gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc
     7981 tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat
     8041 tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc
     8101 accatcgtct tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac
     8161 gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga
     8221 tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa
     8281 accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag cacctaacaa
     8341 aacggcatca accttcttgg aggcttccag cgcctcatct ggaagtggga cacctgtagc
     8401 atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac
     8461 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc
     8521 acctggcaaa acgacgatct tcttaggggc agacataggg gcagacatta gaatggtata
     8581 tccttgaaat atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag
     8641 acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca
     8701 agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt
     8761 ccggcctctc acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag
     8821 gcgacctctg aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc
     8881 ccctgtgtgt tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct
     8941 tgcatcttac gatacctgag tattcccaca gttaactgcg gtcaagatat ttcttgaatc
     9001 aggcgcctta gaccgctcgg ccaaacaacc aattacttgt tgagaaatag agtataatta
     9061 tcctataaat ataacgtttt tgaacacaca tgaacaagga agtacaggac aattgatttt
     9121 gaagagaatg tggattttga tgtaattgtt gggattccat ttttaataag gcaataatat
     9181 taggtatgta gatatactag aagttctcct cgaca
//
