LOCUS       V001865                 4921 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001865
VERSION     V001865
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN.
  TITLE     Metabolic Engineering of Cofactor F(420) Production in Mycobacterium
            smegmatis
  JOURNAL   PLoS ONE 5 (12), E15803 (2010)
   PUBMED   21209917
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Bashiri G, Baker EN.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-SEP-2010) School of Biological Sciences, The
            University of Auckland, 3a Symonds St., Auckland, Auckland 1010, New
            Zealand
REFERENCE   3  (bases 1 to 4921)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4921)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2010"; volume: "5"; issue: "12"; pages: "E15803"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-SEP-2010) School of Biological Sciences, The University of
            Auckland, 3a Symonds St., Auckland, Auckland 1010, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        61..79
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             187..204
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             208..240
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             289..306
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      373..420
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      457..885
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             join(3928..4921,1..2)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      181 agcggcctgg tgccgcgcgg cagccatatg gctagcatga ctggtggaca gcaaatgggt
      241 cgggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      301 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      361 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      421 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatatcta
      901 gatttaaaga tctggtaccg cggccgcgcc acggatgcca ccacaagcac tacaacggag
      961 ttcgccacgt acatcaccac aaccaccgat tctggcggtg agctccacga tattcagcgg
     1021 aaatggcttg gtatcgacca agattcgtag aaccccgtct cgtctggctg gtattcaaaa
     1081 cggacgcaac gaaacacgca acgagacagg catggcccaa accagaaaac tagcgtctac
     1141 caggactttt acctgtccga cccgttgcaa cggaaccccc cacggaaccc ccgcgacacc
     1201 cgctccccaa ttgcgttaga acagcggtgg attgtcggct tcgttgtggg ccttttgagc
     1261 cgcttcctgt tctgccgcac gctctttcct cgcccgatag ccgagtcgct taacggtgtc
     1321 cagatgcagc ccgaaatgtt tggccgtttg cggccaagag tggccctcgt cgtcgtgata
     1381 ggcgcggatg cgttcgcggc gtgcagcctg ctcggcgaac cactcgctgc gttcctgcgc
     1441 cacgagccgg acgacgtggc gttcggatag tccggtgatt cgagcgcctt cggcggcggt
     1501 cacgcgccgc tttttgcgga cagtcggctg ccagttgtag ccgtcgctgt agccgtcgct
     1561 gtagccgtcg ctcatagcaa tgcctccatg catggctgac gcggactttg cgcgccgcgc
     1621 aactgtgctc gccgccgtgc gcgctgctgc gcccttccgc gagatggccg catggcgcgc
     1681 actgagtgtg gcctcgtaga ccacgatccc gtccgcccaa atgcgcgact tggttgtgat
     1741 ccaacgccaa atgctgttgg cgatggcgcg gacctcgctg tccggtagcg gtccgggaca
     1801 cacgtcgttg cacggaaatt cggcgtttcg cgcgtggcac tcggcataga tcgcgcggcc
     1861 gagtccgtcc acgttccggg tcggcaggta gatccgcatg agggcgggaa gataggccca
     1921 caacctgacg gaatcgaaca gtgcgcaatt ccgccctagc ggcgtcggag ccgctttgta
     1981 cgtggtctgc tgacgccagc gcggcggtgg catgttcgcg ccgagctcgg cctcgatgtg
     2041 gctgagtgtg tagagatctg agtggagcca ttccgtttcc caggcgatgt ggccggggtt
     2101 tttggtcatg aggcctgagt aactgcggtc gccatcgacg gcgcgccgaa ggccttcggc
     2161 gcacgccgcc atgtatgcga gcggcttacg ccgcgcgtat tcggtgcgtg gaacaggggc
     2221 gttgagtgcc cacactgcgt gtgcgtggcc gttggcgcga ttgcccacga tcgcgttggg
     2281 cagcggatgg gacccccggg cgctgagcgc tcggagcgct gcgtctggat ggtctacgtc
     2341 cacgaccagc aggtttgcca gcgctgttgg gttcgcctcg atgtaccggc ggcctagggc
     2401 cgacgcgcgg ctttggcggt agatcccctc gagcagatcg tcgcttgcca gggaccagta
     2461 cggcagccag agctgctcaa attcgtcggc gacgtggctc acgcttgata ataaaccacg
     2521 attaatcacc ggtgtatggt ccgacacgag ctccaagtca gatatttcgc tgaggggcca
     2581 ccccacaact gcacactccc ccgctctccc gtcgagccct gatgatgaaa caccagcgac
     2641 agccgagcac ccccaaccac ctgtaccaac caggaggaac acatgcgtcg tttcgaggac
     2701 gtttccgggc cgctaagagc cgctgtggcg gccgtacacg ccgccttaga cccgttagac
     2761 cccctgccgc ctgaatgcgc gggtacgagc cacacagcac ccgaacttac ggagctggtg
     2821 ggctcacgcc gtgcggccgc acgcgttgcg ctcggtcgtt cggctgcggc gagcggtatc
     2881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3181 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3601 gatcttttct acggggtctg acgctcagtg gaactagagc atgagattat caaaaaggat
     3661 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3721 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3781 tctatttcgt tcatccatag ttgcctgact ccccgacgtg gccgaccagc ccgtcatcgt
     3841 caacgcctga ccgcggtgcg gacaggccgt gtcgcgaccg gccgtgcgga attaagccgg
     3901 cccgtaccct gtgaatagag gtccgctgtg acacaagaat ccctgttact tctcgaccgt
     3961 attgattcgg atgattccta cgcgagcctg cggaacgacc aggaattctg ggagccgctg
     4021 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
     4081 gagagcacca accccgtact ggtcggcgag cccgacccgg tgatcaagct gttcggcgag
     4141 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
     4201 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
     4261 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
     4321 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     4381 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     4441 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     4501 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     4561 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     4621 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     4681 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     4741 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     4801 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     4861 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     4921 g
//
