LOCUS       40924_48273        5298 bp DNA     circular SYN 27-JUL-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Pastrana DV, Buck CB, Pang YY, Thompson CD, Castle PE, FitzGerald 
            PC, Kruger Kjaer S, Lowy DR, Schiller JT
  TITLE     Reactivity of human sera in a sensitive, high-throughput 
            pseudovirus-based papillomavirus neutralization assay for HPV16 and 
            HPV18.
  JOURNAL   Virology. 2004 Apr 10;321(2):205-16.
  PUBMED    15051381
REFERENCE   2  (bases 1 to 5298)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5298)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2004-04-10"; volume: "321(2)"; pages: "205-16"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        681..1859
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1940..3448
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="PTMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKL
                     QPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNV
                     DKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVG
                     VVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGG
                     RKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSV
                     THLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHE
                     SRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPG
                     KARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAV
                     FARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     polyA_signal    complement(3506..3627)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4046..4634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4666..5061)
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5080..5127)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     promoter        complement(join(5175..5298,1..206))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
       61 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
      121 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      181 gactttccac accctaactg acacacattc cacagaattg cgttgacatt gattattgac
      241 tagttagtta ttactagccg cgtgcaaagc accggcggcg gtagatgcgg ggtaagtact
      301 gaattttaat tcgacctatc ccggtaaagc gaaagcgaca cgcttttttt tcacacatag
      361 cgggaccgaa cacgttataa gtatcgaact cctaaagaag cagtgtagtt ttctgcagat
      421 gcaaaaaaga tctcatgaag gaggaacttg tgcagtttac ttaatctgct ttaacacagc
      481 taaaagcaga gaaacagtcc ggaatctgat ggcaaacatg ctaaatgtaa gagaagagtg
      541 tttgatgctg cagccaccta aaattcgagg actcagcgca gctctattct ggtttagctt
      601 tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg
      661 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      721 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
      781 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
      841 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
      901 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
      961 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     1021 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     1081 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     1141 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     1201 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     1261 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     1321 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     1381 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     1441 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     1501 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     1561 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     1621 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     1681 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     1741 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     1801 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     1861 gaattctcta gagatccctc gacctcgaga tccattgtgc tggatctgcg atctaagtaa
     1921 gcttcgaatc gcgaattcgc ccaccatgct gctgctgctg ctgctgctgg gcctgaggct
     1981 acagctctcc ctgggcatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
     2041 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
     2101 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
     2161 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
     2221 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
     2281 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
     2341 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
     2401 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
     2461 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
     2521 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
     2581 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
     2641 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
     2701 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
     2761 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
     2821 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
     2881 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
     2941 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
     3001 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
     3061 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
     3121 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
     3181 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
     3241 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
     3301 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
     3361 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     3421 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggttactcta gagtcggggc
     3481 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     3541 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3601 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3661 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     3721 atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3781 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3841 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtttagccct cccacacata
     4681 accagagggc agcaattcac gaatcccaac tgccgtcggc tgtccatcac tgtccttcac
     4741 tatggctttg atcccaggat gcagatcgag aagcacctgt cggcaccgtc cgcaggggct
     4801 caagatgccc ctgttctcat ttccgatcgc gacgatacaa gtcaggttgc cagctgccgc
     4861 agcagcagca gtgcccagca ccacgagttc tgcacaaggt cccccagtaa aatgatatac
     4921 attgacacca gtgaagatgc ggccgtcgct agagagagct gcgctggcga cgctgtagtc
     4981 ttcagagatg gggatgctgt tgattgtagc cgttgctctt tcaatgaggg tggattcttc
     5041 ttgagacaaa ggcttggcca tggtttagtt cctcaccttg tcgtattata ctatgccgat
     5101 atactatgcc gatgattaat tgtcaacacg tgctgatcag atccgaaaat ggatatacaa
     5161 gctcccggga gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     5221 aatagctcag aggcagaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     5281 tggggcggag aatgggcg
//
