LOCUS       40924_48258       11783 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gene deletion vector pYS1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Shenkerman Y, Elharar Y, Vishkautzan M, Gur E.
  TITLE     Efficient and simple generation of unmarked gene deletions in 
            Mycobacterium smegmatis
  JOURNAL   Gene 533 (1), 374-378 (2014)
  PUBMED    24100088
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Shenkerman Y, Elharar Y, Vishkautzan M, Gur E.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2013) Life Sciences, Ben-Gurion University of the 
            Negev, Rager Blvd, Beer-Sheva 84105, Israel
REFERENCE   3  (bases 1 to 11783)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11783)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "2014"; volume: "533"; issue: "1"; pages: "374-378"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-AUG-2013) Life Sciences, Ben-Gurion University of the Negev, 
            Rager Blvd, Beer-Sheva 84105, Israel"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        204..648
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             649..2043
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILE"
     rep_origin      complement(2210..2798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2964..5717
                     /label=mycobacterial PAL5000ts replication origin
                     /note="mycobacterial PAL5000ts replication origin"
     CDS             complement(5956..6768)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      complement(6929..6975)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(7040..8101)
                     /codon_start=1
                     /gene="che9c 61"
                     /product="Che9c 61"
                     /label=che9c 61
                     /protein_id="AGY30910.1"
                     /translation="MAENAVTKQDSPKAPETISQVLQVLVPQLARAVPKGMDPDRIARI
                     VQTEIRKSRNAKAAGIAKQSLDDCTQESFAGALLTSAALGLEPGVNGECYLVPYRDTRR
                     GVVECQLIIGYQGIVKLFWQHPRASRIDAQWVGANDEFHYTMGLNPTLKHVKAKGDRGN
                     PVYFYAIVEVTGAEPLWDVFTADEIRELRRGKVGSSGDIKDPQRWMERKTALKQVLKLA
                     PKTTRLDAAIRADDRPGTDLSQSQALALPSTVKPTADYIDGEIAEPHEVDTPPKSSRAQ
                     RAQRATAPAPDVQMANPDQLKRLGEIQKAEKYNDADWFKFLADSAGVKATRAADLTFDE
                     AKAVIDMFDGPNA"
     gene            complement(7040..8101)
                     /gene="che9c 61"
                     /label=che9c 61
     CDS             complement(8094..9038)
                     /codon_start=1
                     /gene="che9c 60"
                     /product="Che9c 60"
                     /label=che9c 60
                     /protein_id="AGY30909.1"
                     /translation="MSVPTQDGMHRFVDEDVYHADRGSLSVSGAKLLLPPSCPAKFRWE
                     MDNTRKPKKVWDFGHVAHKLVLGKGAEFEILDPEVHGLKADGTPSEKPTATGMWRKAEA
                     EARKQGKVPIHVDLFTKAYDMAEKVRQHPTAGPIFANPDGEAEVALYYTDPETGVRLRG
                     RIDWLTDDIDDYKTSMTANPAELKTKFYKLGYFMQAAWYIDLLVALGLAENPRFRFITQ
                     EKEPPYVVTPIQYDDEAIEEGRRRNRQAIRLYADCMESGKWPDYSDDVVTISLPSWGLP
                     RPQTVGDVVTDSYIYDTDPLEEADPIEGDYIYG"
     gene            complement(8094..9038)
                     /gene="che9c 60"
                     /label=che9c 60
     CDS             complement(9127..9768)
                     /codon_start=1
                     /gene="amiS"
                     /product="AmiS acetamidase regulator"
                     /label=amiS
                     /protein_id="AGY30908.1"
                     /translation="MGGVGLFYVGAVLIIDGLMLLGRISPRGATPLNFFVGGLQVVTPT
                     VLILQSGGDAAVIFAASGLYLFGFTYLWVAINNVTDWDGEGLGWFSLFVAIAALGYSWH
                     AFTAEADPAFGVIWLLWAVLWFMLFLLLGLGHDALGPAVGFVAVAEGVITAAVPAFLIV
                     SGNWETGPLPAAVIAVIGFAAVVLAYPIGRRLAAPSVTNPPPAALAATTR"
     gene            complement(9127..9768)
                     /gene="amiS"
                     /label=amiS
     CDS             complement(9773..10057)
                     /codon_start=1
                     /gene="amiD"
                     /product="AmiD acetamidase regulator"
                     /label=amiD
                     /protein_id="AGY30907.1"
                     /translation="MPTYTFRCSHCGPFDLTCAISERDAAATCPECRTPARRVFGSVGL
                     TTFTAGHHRAFDAASASAESPTVVKSIPAGADRPRAPRRNPGLPSLPRY"
     gene            complement(9773..10057)
                     /gene="amiD"
                     /label=amiD
     CDS             complement(10137..10565)
                     /codon_start=1
                     /gene="amiA"
                     /product="AmiA acetamidase regulator"
                     /label=amiA
                     /protein_id="AGY30906.1"
                     /translation="MAAGQQRRPNLLLPLVRLTHLAESAIERVLADSSLKIEDWRVLDE
                     LAGRRTVPMSDLAQATLITGPTLTRTVDRLVSQGIIYRTADLHDRRRVLVALTPRGRTL
                     RNRLVDAVAEAECAAFESCGLDVDQLRELVDTTSNLTS"
     gene            complement(10137..10565)
                     /gene="amiA"
                     /label=amiA
     CDS             10668..11741
                     /codon_start=1
                     /gene="amiC"
                     /product="AmiC acetamidase regulator"
                     /label=amiC
                     /protein_id="AGY30905.1"
                     /translation="MQDGEVEFRVGLVIPLQGPAGIFAPSCEAVAELAAKEVNDRGGLQ
                     GRKVTIEVLDGGRPGDDVARTVADRLRGHGLDAVTGWHISAVRNRISPVVRDRIPYVYT
                     SLYEGGERTPGVFCTGETPQIQIAPALAWLRDHFGIRSWCLVGDDYIWPRRSAAAARAY
                     CRDLDLELRREIYVPYGTDDFRAPVRKAIASGAQAVLMLLVGQDAVLFNREFARAGGHD
                     RMARFSPLMEENMLLASGAGSTENLYVAAAYFSSLATAGAMDLMGSYVARYGADAPPLN
                     AMAESCYEGLVALEAIFQRAHSPEIPDLMASAHDVGFDGPRGPMCMRDSQFDQQVYIAS
                     ADGYDFDILDTLTTLDA"
     gene            10668..11741
                     /gene="amiC"
                     /label=amiC
ORIGIN
        1 ggtaccgata tctgatttag gatacatttc tagccacctg acgtcggggg ggggggggga
       61 aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      121 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      181 tcaacgggaa acgtcttgct cgaccatata cctgccgttc actattattt agtgaaatga
      241 gatattatga tattttctga attgtgatta aaaaggcaac tttatgccca tgcaacagaa
      301 actataaaaa atacagagaa tgaaaagaaa cagatagatt ttttagttct ttaggcccgt
      361 agtctgcaaa tccttttatg attttctatc aaacaaaaga ggaaaataga ccagttgcaa
      421 tccaaacgag agtctaatag aatgaggtcg aaaagtaaat cgcgcgggtt tgttactgat
      481 aaagcaggca agacctaaaa tgtgtaaagg gcaaagtgta tactttggcg tcacccctta
      541 catattttag gtcttttttt attgtgcgta actaacttgc catcttcaaa caggagggct
      601 ggaagaagca gaccgctaac acagtacata aaaaaggaga catgaacgat gaacatcaaa
      661 aagtttgcaa aacaagcaac agtattaacc tttactaccg cactgctggc aggaggcgca
      721 actcaagcgt ttgcgaaaga aacgaaccaa aagccatata aggaaacata cggcatttcc
      781 catattacac gccatgatat gctgcaaatc cctgaacagc aaaaaaatga aaaatatcaa
      841 gttcctgaat tcgattcgtc cacaattaaa aatatctctt ctgcaaaagg cctggacgtt
      901 tgggacagct ggccattaca aaacgctgac ggcactgtcg caaactatca cggctaccac
      961 atcgtctttg cattagccgg agatcctaaa aatgcggatg acacatcgat ttacatgttc
     1021 tatcaaaaag tcggcgaaac ttctattgac agctggaaaa acgctggccg cgtctttaaa
     1081 gacagcgaca aattcgatgc aaatgattct atcctaaaag accaaacaca agaatggtca
     1141 ggttcagcca catttacatc tgacggaaaa atccgtttat tctacactga tttctccggt
     1201 aaacattacg gcaaacaaac actgacaact gcacaagtta acgtatcagc atcagacagc
     1261 tctttgaaca tcaacggtgt agaggattat aaatcaatct ttgacggtga cggaaaaacg
     1321 tatcaaaatg tacagcagtt catcgatgaa ggcaactaca gctcaggcga caaccatacg
     1381 ctgagagatc ctcactacgt agaagataaa ggccacaaat acttagtatt tgaagcaaac
     1441 actggaactg aagatggcta ccaaggcgaa gaatctttat ttaacaaagc atactatggc
     1501 aaaagcacat cattcttccg tcaagaaagt caaaaacttc tgcaaagcga taaaaaacgc
     1561 acggctgagt tagcaaacgg cgctctcggt atgattgagc taaacgatga ttacacactg
     1621 aaaaaagtga tgaaaccgct gattgcatct aacacagtaa cagatgaaat tgaacgcgcg
     1681 aacgtcttta aaatgaacgg caaatggtac ctgttcactg actcccgcgg atcaaaaatg
     1741 acgattgacg gcattacgtc taacgatatt tacatgcttg gttatgtttc taattcttta
     1801 actggcccat acaagccgct gaacaaaact ggccttgtgt taaaaatgga tcttgatcct
     1861 aacgatgtaa cctttactta ctcacacttc gctgtacctc aagcgaaagg aaacaatgtc
     1921 gtgattacaa gctatatgac aaacagagga ttctacgcag acaaacaatc aacgtttgcg
     1981 ccaagcttcc tgctgaacat caaaggcaag aaaacatctg ttgtcaaaga cagcatcctt
     2041 gaagcggccg ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     2101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     2161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2641 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2821 cggggtctga cgctcagtgg aacgagtatc gagccgagaa cgttatcgaa gttggtcatg
     2881 tgtaatcccc tcgtttgaac tttggattaa gcgtagatac acccttggac aagccagttg
     2941 gattcggaga caagcaaatt cagccttaaa aagggcgagg cctgcggtgg tggaacaccg
     3001 cagggcctct aaccgctcga cgcgctgcac caaccagccc gcgaacggct ggcagccagc
     3061 gtaaggcgcg gctcatcggg cggcgttcgc cacgatgtcc tgcacttcga gccaagcctc
     3121 gaacacctgc tggtgtgcac gactcacccg gttgttgaca ccgcgcgcgg ccgtgcgggc
     3181 tcggtggggc ggctgtgtcg cccttgccag cgtgagtagc gcgtacctca cctcgcccaa
     3241 caggtcgcac acagccgatt cgtacgccat aaagccaggt gagcccacca gctccgtaag
     3301 ttcgggcgct gtgtggctcg tacccgcgca ttcaggcggc agggggtcta acgggtctaa
     3361 ggcggcgtgt acggccgcca cagcggctct cagcggcccg gaaacgtcct cgaaacgacg
     3421 catgtgttcc tcctggttgg tacaggtggt tgggggtgct cggctgtcgc tggtgttcca
     3481 ccaccagggc tcgacgggag agcgggggag tgtgcagttg tggggtggcc cctcagcgaa
     3541 atatctgact tggagctcgt gtcggaccat acaccggtga ttaatcgtgg tctactacca
     3601 agcgtgagcc acgtcgccga cgaatttgag cagctctggc tgccgtactg gccgctggca
     3661 agcgacgatc tgctcgaggg gatctaccgc caaagccgcg cgtcggccct aggccgccgg
     3721 tacatcgagg cgaacccaac agcgctggca aacctgctgg tcgtggacgt agaccatcca
     3781 gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc cgctgcccaa cgcgatcgtg
     3841 ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac tcaacgcccc tgttccacgc
     3901 accgaatacg cgcggcgtaa gccgctcgca tacatggcgg cgtgcgccga aggccttcgg
     3961 cgcgccgtcg acggcgaccg cagttactca ggcctcatga ccaaaaaccc cggccacatc
     4021 gcctgggaaa cggaatggct ccactcagat ctctacacac tcagccacat cgaggccgag
     4081 ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga ccacgtacaa agcggctccg
     4141 acgccgctag ggcggaattg cgcactgttc gattccgtca ggttgtgggc ctatcgtccc
     4201 gccctcatgc ggatctacct gccgacccgg aacgtggacg gactcggccg cgcgatctat
     4261 gccgagtgcc acgcgcgaaa cgccgaattc ccgtgcaacg acgtgtgtcc cggaccgcta
     4321 ccggacagcg aggtccgcgc catcgccaac agcatttggc gttggatcac aaccaagtcg
     4381 cgcatttggg cggacgggat cgtggtctac gaggccacac tcagtgcgcg ccagtcggcc
     4441 atctcgcgga agggcgcagc agcgcgcacg gcggcgagca cagttgcgcg gcgcgcaaag
     4501 tccgcgtcag ccatggaggc attgctatga gcgacggcta cagcgacggc tacagcgacg
     4561 gctacaaccg gcagccgact gtccgcaaaa agcggcgcgt gaccgccgcc gaaggcgctc
     4621 gaatcaccgg actatccgaa cgccacgtcg tccggctcgt ggcgcaggaa cgcagcgagt
     4681 ggctcgccga gcaggctgca cgccgcgaac gcatccgcgc ctatcacgac gacgagggcc
     4741 actcttggcc gcaaacggcc aaacatttcg ggctgcatct ggacaccgtt aagcgactcg
     4801 gctatcgggc gaggaaagag cgtgcggcag aacaggaagc ggctcaaaag gcccacaacg
     4861 aagccgacaa tccaccgctg ttctaacgca attggggagc gggtgtcgcg ggggttccgt
     4921 ggggggttcc gttgcaacgg gtcggacagg taaaagtcct ggtagacgct agttttctgg
     4981 tttgggccat gcctgtctcg ttgcgtgttt cgttgcgccc gttttgaata ccagccagac
     5041 gagacggggt tctacgaatc ttggtcgata ccaagccatt tccgctgaat atcggggagc
     5101 tcaccgccag aatcggtggt tgtggtgatg tacgtggcga actccgttgt agtgcctgtg
     5161 gtggcatccg tggccactct cgttgcacgg ttcgttgtgc cgttacaggc cccgttgaca
     5221 gctcaccgaa cgtagttaaa acatgctggt caaactaggt ttaccaacga tacgagtcag
     5281 ctcatctagg gccagttcta ggcgttgttc gttgcgcggt tcgttgcgca tgtttcgtgt
     5341 ggttgctaga tggctccgca accacacgct tcgaggttga gtgcttccag cacgggcgcg
     5401 atccagaaga acttcgtcgt gcgactgtcc tcgttaacgc tcgaagcgat gccgatcaaa
     5461 tcggctgtgc cgtcgcggtt cttgcggtac agcggcgcgc ctgagtcgcc atgtacggtg
     5521 cgcagctgag cggtgatcgt gtacttggca gccacaacga ccggcccaca ggtctcgccg
     5581 gatttcatcc cgtacttgca gacctcggtg ccgtacggaa catctttgcg gtccttcagc
     5641 accgcacgca cggggtatct tccgccgatt ttgaacgaag cgatcggtgt tcccggagca
     5701 aacgcaatca ccccagcgtc ggtgtagacc ggcaatagat catcgtcgcc ggtaatcgtg
     5761 gtgccttcgt ctctcgacgc aacgtaggta cccaacgtcc gctgtgtatc cggtttctct
     5821 gcaaccgaat acagcaccgg gccccccctc gagtttaaac atttttctaa atacattcaa
     5881 atatgtatcc gctcatgaga caataaccat gcatgtgcta gcccagtgtt acaaccaatt
     5941 aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     6001 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     6061 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     6121 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     6181 atgagtgacg actgaatccg gtgagaatgg caaaagatta tgcatttctt tccagacttg
     6241 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     6301 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     6361 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     6421 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     6481 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     6541 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     6601 atgtttcaga aacaactctg gcgcatgggg cttcccatac aagcgataga ttgtcgcacc
     6661 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     6721 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     6781 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     6841 aatgtaacat cagagatttt gagacacaac gtggctttcc cccccccccg acgtcaggtg
     6901 gctagctgat caccgcggcc atgatggcat aaaacgaaag gcccagtctt tcgactgagc
     6961 ctttcgtttt atttgatgcc tggcagtcga tcgtacgcta gttgcagatc aaccaccgcg
     7021 tcggaattcg ccggggcgct catgcgttgg gcccgtcgaa catgtcgatg acagccttcg
     7081 cctcgtcgaa cgtgaggtcg gcggcgcgcg tggctttgac gccggccgaa tcggcgagga
     7141 acttgaacca atcggcgtcg ttgtactttt cggccttctg gatctcgccg aggcgcttca
     7201 gctgatcggg attggccatc tgcacgtcgg gcgccggcgc ggtggcgcgc tgcgcgcgtt
     7261 gtgcgcgcga cgacttcggt ggcgtatcga cctcgtgcgg ctcggcgatc tcgccgtcga
     7321 tgtagtcggc cgtcggcttg acggtcgacg gcagcgcgag cgcctgcgac tgtgacaggt
     7381 cggtgcccgg gcgatcgtcg gcgcggatcg ccgcgtcgag ccgcgtcgtc ttcggtgcca
     7441 gcttcagcac ctgtttgagc gcggtcttcc gctccatcca gcgctgcggg tccttgatgt
     7501 cgcccgagga tccgaccttg ccgcgacgca attccctgat ctcgtcggcg gtgaacacgt
     7561 cccacagcgg ctcagcgccg gtcacctcga cgatcgcgta gaagtagacc ggattacccc
     7621 gatcaccctt ggccttcacg tgtttcagcg tcggattgag gcccatggtg taatggaatt
     7681 cgtcgttcgc gccgacccac tgcgcgtcga tccgggaggc gcgcgggtgc tgccagaaca
     7741 gtttgacgat gccctggtag ccgatgatca gctggcactc gaccacgccg cgccgggtgt
     7801 cgcggtaggg cacgaggtag cactcgccgt tgacaccggg ctcgagaccg agcgcggccg
     7861 aggtcagcag cgcaccggca aatgactctt gcgtgcagtc gtcgagggac tgcttagcga
     7921 tgccagcagc tttcgcgttg cgcgacttgc ggatctcggt ttgcacgatc cgcgcgatgc
     7981 ggtcggggtc catacccttg ggcactgcac gcgccagctg cggcacgagc acctgcagca
     8041 cttgcgagat cgtctcgggt gccttgggcg aatcctgctt ggtgacagca ttttcagcca
     8101 tagatgtaat ccccttcaat cgggtcggcc tcttcgagcg ggtcggtgtc gtagatatag
     8161 ctgtcggtga cgacgtcgcc gacggtctgc ggtcgcggca gcccccacga gggcaggctg
     8221 atcgtgacca cgtcgtcgct gtagtcaggc cacttgcccg attccatgca gtcggcgtag
     8281 agccggatcg cctggcggtt gcggcgccgc ccctcttcga tcgcctcgtc gtcgtactgg
     8341 atcggagtca cgacgtaggg cggttctttc tcctgcgtga tgaaccggaa tcgcgggttc
     8401 tcggcgagcc cgagggcgac cagtagatcg atgtaccagg ccgcctgcat gaaatagccg
     8461 agcttgtaga acttggtttt cagctcggcc gggttcgcgg tcatcgacgt cttgtaatca
     8521 tcgatatcgt cagtgagcca gtcgatccgg ccacgcagcc gcacgccggt ctcggggtcg
     8581 gtgtagtaca gcgcgacctc ggcctcgccg tcaggattgg cgaagatcgg gccggctgtc
     8641 gggtgctgac gcaccttttc ggccatgtcg tacgccttcg tgaacaggtc gacgtgaatc
     8701 ggcaccttgc cctgtttgcg agcctcagcc tcggccttgc gccacatgcc cgtcgcggtc
     8761 ggcttctccg acggcgtacc gtccgccttc agcccgtgca cctcggggtc gaggatctcg
     8821 aactcggcac ccttgccgag caccagtttg tgcgcgacat gtccgaagtc ccagaccttt
     8881 ttcggcttcc gggtgttgtc catctcccag cggaatttcg cgggacacga cggcggcaac
     8941 agcagcttcg cgccggatac cgacagcgag ccccggtcag cgtggtagac gtcctcgtcg
     9001 acgaaccggt gcattccgtc ctgtgtgggc acactcatat gcggccgccg atctcgtaac
     9061 tacgtcgaca tcgataagct tcgaattctg cagctggatc cgttacatat gtggactccc
     9121 tttctcttat cgggtggtgg ccgcgagcgc ggccggtgga gggttggtga ctgacggcgc
     9181 tgcgagacgg cgcccgatgg ggtatgcgag aacaactgcg gcaaaaccga tcacggcgat
     9241 gaccgcggcg gggagcgggc cggtttccca gttgcccgac acgatcagga aggccggcac
     9301 ggcggcggtg atcacgcctt cggccaccgc gacgaacccg acggcgggcc ccagtgcgtc
     9361 gtgccccagg ccgagcagca ggaacagcat gaaccacagc actgcccaca gcagccagat
     9421 caccccgaac gccgggtcgg cctcggcggt gaacgcgtgc cacgagtagc cgagtgcggc
     9481 gatcgcgacg aacagcgaga accatccgag accttctccg tcccagtcgg tcacgttgtt
     9541 gatggccacc cacaggtagg tgaagccgaa caggtagagc ccggaggccg cgaagatcac
     9601 ggccgcgtct ccgccggact gcaggatcag caccgtaggc gtcaccacct gcagtccgcc
     9661 gacgaagaag ttcagcggtg ttgcgcctcg tgggctgatg cggcccagca gcatcagccc
     9721 gtcgatgatg agcaccgcac ccacgtagaa gagcccgacg ccacccatgt cgctagtacc
     9781 tcggcagact cggtagaccg ggattgcggc gcggggcccg cgggcggtcc gcgcctgcgg
     9841 gaatcgactt caccaccgtg ggactttcgg cgctcgcgga cgccgcgtcg aatgcgcggt
     9901 gatgtcccgc ggtgaatgtc gtcagcccta ccgaaccgaa gacccggcgc gccggcgtcc
     9961 ggcactccgg acaggtcgcc gccgcatcgc gctcggagat cgcgcaggtg agatcgaagg
    10021 gaccgcagtg ggaacaacgg aatgtgtagg tcggcatggt cacccctttc cattcacccg
    10081 aaatatatcc agatgaaaat aaaagtcaag ggtgaacgcc ggccgggcgc gggtggttac
    10141 gaagtcaaat tcgaggtggt gtcgacgagt tcgcgcaact ggtcgacgtc cagcccgcac
    10201 gattcaaaag ccgcacactc ggcctcggct accgcgtcca ccaggcggtt gcgcagcgtc
    10261 cgcccccgcg gggtcaacgc cacgagcacc cgccggcggt catgcagatc ggcagtccgg
    10321 tagatgatcc cttgcgacac aaggcgatcg acggttctgg tgagagtcgg acccgtgatc
    10381 agcgtggcct gcgcgagatc gctcatgggc acggtgcgcc gtccggccaa ctcgtcgagc
    10441 acgcgccagt cctcgatctt gagcgacgag tccgcgagca cgcgttcgat cgccgactcc
    10501 gcgaggtggg tcagacgcac caacggcagc aggaggttgg ggcggcgctg ctgtccggcg
    10561 gccatggtgg gtgtctcctc gtgtcgacgg cggggtctgt tcagaatgaa ttatttctgc
    10621 tagaaagctt tgaagcgcag actacacggg atcgacggtg atctggagtg caggacggcg
    10681 aggttgagtt ccgcgtcggg ctggtgattc cgcttcaagg gccagcggga atcttcgcgc
    10741 cttcgtgcga ggccgtggcc gagctcgcgg ccaaggaggt caacgaccga ggcggcctgc
    10801 agggccgcaa ggtcaccatc gaggtgctcg acggcggacg gcccggcgac gacgtcgccc
    10861 ggaccgtcgc cgaccggttg cgaggtcacg gtctcgacgc cgtgaccggc tggcacatct
    10921 cggccgtgcg caaccgcatc tccccggtgg tccgcgaccg catcccgtac gtctacacct
    10981 cgttgtacga gggcggtgaa cgcacaccgg gcgtgttctg cacaggcgag acaccgcaga
    11041 tccagatcgc gcccgcgctc gcctggctgc gcgaccactt cggcatccgg tcctggtgcc
    11101 tggtcggcga tgactacatc tggccgcgcc gttccgccgc ggccgcccgc gcgtactgcc
    11161 gagatctgga cctcgagctc agacgggaga tctacgtccc gtacggcacc gacgatttcc
    11221 gcgcaccggt ccgcaaggcc atcgcgtcgg gggcgcaggc cgtgttgatg ctgctcgtcg
    11281 gccaggacgc cgtgttgttc aaccgcgagt tcgcgcgcgc cggcgggcac gaccgcatgg
    11341 cgcggttcag cccgctgatg gaggagaaca tgctgctggc cagcggcgcc ggctccaccg
    11401 aaaacctcta tgtcgccgcg gcgtacttca gttcactggc caccgcgggt gccatggacc
    11461 tgatgggcag ctacgtcgcc cgctacggcg ccgacgcgcc accgctcaac gcgatggccg
    11521 aatcctgtta cgagggtctc gttgcgctcg aggccatctt ccagcgggcc cactcccccg
    11581 agataccgga cctgatggca tccgcgcacg atgtcgggtt cgacgggccc cggggcccga
    11641 tgtgtatgcg cgacagtcaa ttcgaccagc aggtctacat cgccagcgcc gacggctacg
    11701 acttcgacat cctcgacacg ctgacgacgc tcgacgcttg agaccgcgtc acttctttat
    11761 ctagatttaa agatctggta ccg
//
