LOCUS       62056_23185        9897 bp DNA     circular SYN 19-JUL-2021
DEFINITION  Biosensor vector pYQS040, complete sequence.
ACCESSION   MW810319
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Sekurova ON, Sun YQ, Zehl M, Ruckert C, Stich A, Busche T, 
            Kalinowski J, Zotchev SB.
  TITLE     Coupling of the engineered DNA 'mutator' to a biosensor as a new 
            paradigm for activation of silent biosynthetic gene clusters in 
            Streptomyces
  JOURNAL   Nucleic Acids Res. (2021) In press
  PUBMED    34197612
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   Sekurova ON, Sun Y-Q., Zehl M, Ruckert C, Stich A, Busche T, 
            Kalinowski J, Zotchev SB.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2021) CeBiTec, Bielefeld University, 
            Universitaetsstr. 27, Bielefeld, NRW 33615, Germany
REFERENCE   3  (bases 1 to 9897)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2021) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2021) CeBiTec, Bielefeld University, Universitaetsstr. 27, 
            Bielefeld, NRW 33615, Germany"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: canu v. 2.1.1
            Sequencing Technology :: Illumina; ONT
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /note="attP; phiC31 attachment site"
     CDS             83..1897
                     /label=phage phi-C31 integrase
                     /note="integrase from phage phi-C31"
     primer_bind     2343..2359
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2517..2552
                     /regulatory_class="promoter"
     misc_feature    2553
                     /label=transcription start site
                     /note="transcription start site"
     gene            2659..6510
                     /gene="bpsA"
                     /label=bpsA
     CDS             2659..6510
                     /codon_start=1
                     /transl_table=11
                     /gene="bpsA"
                     /product="indigoidine synthase"
                     /label=bpsA
                     /protein_id="QXN60064.1"
                     /translation="MTLQETSVLEPTLRGTTTLPDLLAKRVAEHPEATAVAYRDEKLTY
                     RELASRSSALAEYLRHLGVSTDDCVGLFVEPSIDLMVGAWGILSAGAAYLPLSPEYPED
                     RLRYMIENSQAKIILAQQRLVTRLRELAPQDVRVVTLRESEAFVLPEGQVAPAIEGARP
                     DSLAYVIYTSGSTGKPKGVMIEHHSIVSQLGWLRETYGIDRSKTILQKTPMSFDAAQWE
                     ILSPANGATVVMGAPGVYADPEGLIETIVKYGVTTLQCVPTLLQGLLDTEKFPECTSLQ
                     QIFSGGEALSRLLAIQTTQEMPGRALINVYGPTECTINSSSYAVDPAELGEAPQSISIG
                     APVADTEYHILGKEDLKPVGVGEIGELYIGGGQLARGYLHRPDLTAERFLEIEVTEGAG
                     PVRLYKTGDLGQWNPDGTVQFAGRADNQVKLRGYRVELDEISLAIENHDWVRNAAVIVK
                     NDGRTGFQNLIACVELSEKEAALMDQGNHGSHHASKKSKLQVKAQLSNPGLRDDADLAA
                     RVAYDLPGAEPTPEQRSRVFARKTYRFYEGGAVTEADLLALLGGQVPAAYSRKAADLAP
                     AELGQILRWFGQYLSEERLLPKYGYASPGALYATQLYFELEGVGGLQPGYYYYQPQRHQ
                     LVLISEKAATGRPTAHIHFIGKRGGIEPVYKNNIQEVLEIETGHIVGLFEQVLPAYGLD
                     IRDLAYEPAVRDLLDVPEEDFYLGTFELVPHTGRREDHAEVYVQTHGSKVANLPEGQYR
                     YADGTLTRFSDDIVLKKQVIAINQSVYQAASFGISVISRAPEEWMHYVTLGKKLQHLMM
                     NGLGLGFMSSGYSSKTGNPLPASRRIDSVLQANGVESGPSYFFVGGRVSDEQLGHEGMR
                     EDSVHMRGPAELIRDDLVSFLPDYMIPNRVVVFERLPLSANGKIDAKALAASDQVNAEL
                     VERPFVAPRTETEKEIAEVWAKSLRRESVSVQDDFFESGGNSLIAVGLIRELNSRLGVS
                     LPLQSVLESPTVEKLSRRLEREVAQESSRLVRLHAETGKDRPVLCWPGLGGYPMNLRTL
                     AGEIGLGRSFYGIQAHGINEGEAPYATITEMAKADIEAIKELQPKGPYTLWGYSFGARV
                     AFETAYQLEQAGEKVDNLFLIAPGSPTVRAENGKVYGREASFANRAYTTILFSVFTGTI
                     SGPDLEKCLESATDEESFAGFISELKGIDVDLAKRIISVVGQTYEFEYSFRELAERTLA
                     APVTIFKARGDDYSFIENSNGYSAEPPTVIDLDADHYSLLRTPDIGELVKHIRYLLGE"
     regulatory      6526..6573
                     /regulatory_class="terminator"
     primer_bind     complement(6607..6623)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6631..6647)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6655..6685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6700..6721)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7009..7597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             7801..8601
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(9038..9406)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(9439..9548)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(9786..9797)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     protein_bind    9868..9897
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 gccccaactg gggtaacctt tgttgagttc tctcagttgg gggcgtaggg tcgccgacat
       61 gacacaaggg gttgtgaccg gggtggacac gtacgcgggt gcttacgacc gtcagtcgcg
      121 cgagcgcgaa aattcgagcg cagcaagccc agcgacacag cgtagcgcca acgaagacaa
      181 ggcggccgac cttcagcgcg aagtcgagcg cgacgggggc cggttcaggt tcgtcgggca
      241 tttcagcgaa gcgccgggca cgtcggcgtt cgggacggcg gagcgcccgg agttcgaacg
      301 catcctgaac gaatgccgcg ccgggcggct caacatgatc attgtctatg acgtgtcgcg
      361 cttctcgcgc ctgaaggtca tggacgcgat tccgattgtc tcggaattgc tcgccctggg
      421 cgtgacgatt gtttccactc aggaaggcgt cttccggcag ggaaacgtca tggacctgat
      481 tcacctgatt atgcggctcg acgcgtcgca caaagaatct tcgctgaagt cggcgaagat
      541 tctcgacacg aagaaccttc agcgcgaatt gggcgggtac gtcggcggga aggcgcctta
      601 cggcttcgag cttgtttcgg agacgaagga gatcacgcgc aacggccgaa tggtcaatgt
      661 cgtcatcaac aagcttgcgc actcgaccac tccccttacc ggacccttcg agttcgagcc
      721 cgacgtaatc cggtggtggt ggcgtgagat caagacgcac aaacaccttc ccttcaagcc
      781 gggcagtcaa gccgccattc acccgggcag catcacgggg ctttgtaagc gcatggacgc
      841 tgacgccgtg ccgacccggg gcgagacgat tgggaagaag accgcttcaa gcgcctggga
      901 cccggcaacc gttatgcgaa tccttcggga cccgcgtatt gcgggcttcg ccgctgaggt
      961 gatctacaag aagaagccgg acggcacgcc gaccacgaag attgagggtt accgcattca
     1021 gcgcgacccg atcacgctcc ggccggtcga gcttgattgc ggaccgatca tcgagcccgc
     1081 tgagtggtat gagcttcagg cgtggttgga cggcaggggg cgcggcaagg ggctttcccg
     1141 ggggcaagcc attctgtccg ccatggacaa gctgtactgc gagtgtggcg ccgtcatgac
     1201 ttcgaagcgc ggggaagaat cgatcaagga ctcttaccgc tgccgtcgcc ggaaggtggt
     1261 cgacccgtcc gcacctgggc agcacgaagg cacgtgcaac gtcagcatgg cggcactcga
     1321 caagttcgtt gcggaacgca tcttcaacaa gatcaggcac gccgaaggcg acgaagagac
     1381 gttggcgctt ctgtgggaag ccgcccgacg cttcggcaag ctcactgagg cgcctgagaa
     1441 gagcggcgaa cgggcgaacc ttgttgcgga gcgcgccgac gccctgaacg cccttgaaga
     1501 gctgtacgaa gaccgcgcgg caggcgcgta cgacggaccc gttggcagga agcacttccg
     1561 gaagcaacag gcagcgctga cgctccggca gcaaggggcg gaagagcggc ttgccgaact
     1621 tgaagccgcc gaagccccga agcttcccct tgaccaatgg ttccccgaag acgccgacgc
     1681 tgacccgacc ggccctaagt cgtggtgggg gcgcgcgtca gtagacgaca agcgcgtgtt
     1741 cgtcgggctc ttcgtagaca agatcgttgt cacgaagtcg actacgggca gggggcaggg
     1801 aacgcccatc gagaagcgcg cttcgatcac gtgggcgaag ccgccgaccg acgacgacga
     1861 agacgacgcc caggacggca cggaagacgt agcggcgtag cgagacaccc gggaagcctg
     1921 atctacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     1981 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     2041 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     2101 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattta tgcggtgtga
     2161 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
     2221 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     2281 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     2341 ttgtaaaacg acggccagtg ccaagcttgg gctgcaggtc gactctagag gatccgtcgt
     2401 gctccgtggt cgcgatcgtc gagccccaag gcgggataag tggcacccca gtgtcacgtg
     2461 ccgcccccac cgggcagtaa tcgcgcgacc accacgcgct ccctctggcg gatctcatgc
     2521 tgtccaccag cggcagggtc caccctggtc ccgtctgctc cggtcctggc ccgcaccgcg
     2581 tcgggttcgc cgcgggcggg ccgcactcgt cgaacggtaa ggggaaaagg cgactagtcg
     2641 agcaacggag gtacggacat gactcttcag gagaccagcg tgcttgagcc caccctgcgc
     2701 ggcaccacca cgttgcccga cctgctggcg aagcgggtcg cggagcaccc agaggcgacc
     2761 gccgtcgcct accgggacga gaagctcacc taccgcgagc tggcgtccag aagctccgcc
     2821 ctcgccgagt acctcagaca cctcggggtc tcgacggacg actgcgtcgg cctcttcgtc
     2881 gagccgtcca tcgacctgat ggtcggcgcc tggggcatcc tgtccgccgg cgccgcctac
     2941 ctgccgctgt ccccggagta ccccgaggac cggctccgct acatgatcga gaacagccag
     3001 gcgaagatca tcctggcgca gcagcgcctg gtcacccgcc tgcgcgagct ggccccgcag
     3061 gacgtcaggg tcgtcaccct gcgcgagtcc gaggcgttcg tcctgcccga gggccaggtg
     3121 gccccggcca tcgagggcgc ccgccccgac agcctcgcct acgtcatcta cacctccggc
     3181 agcacgggca agccgaaggg cgtgatgatc gagcaccaca gcatcgtcag ccagctcggc
     3241 tggctgcgcg agacctacgg catcgaccgc agcaagacca tcctgcagaa gaccccgatg
     3301 agcttcgacg ccgcccagtg ggagatcctc tccccggcca acggtgccac cgtcgtcatg
     3361 ggcgcccccg gcgtctacgc cgaccccgag ggcctcatcg agaccatcgt caagtacggc
     3421 gtgaccaccc tgcagtgcgt ccccaccctg ctccagggcc tgctcgacac cgagaagttc
     3481 cccgagtgca cctccctgca gcagatcttc agcggtggcg aggccctctc gcgcctcctg
     3541 gcgatccaga ccacgcagga gatgcccggc cgcgccctga tcaacgtcta cgggccgacc
     3601 gagtgcacca tcaactcctc ctcgtacgcg gtcgaccccg ccgagctggg cgaggcgccg
     3661 cagtccatct ccatcggcgc cccggttgcc gacaccgagt accacatcct cggcaaggag
     3721 gacctcaagc ccgtcggcgt cggcgagatc ggcgagctgt acatcggcgg cggtcagctg
     3781 gcgcgcggct acctgcaccg ccccgacctg accgccgagc gcttcctgga gatcgaggtc
     3841 accgagggcg ccggtccggt ccgcctctac aagacgggcg acctgggcca gtggaacccg
     3901 gacggcaccg tgcagttcgc cggccgcgcc gacaaccagg tcaagctgcg cggctaccgc
     3961 gtcgagctgg acgagatatc cctggcgatc gagaaccacg actgggtccg caacgccgcc
     4021 gtcatcgtca agaacgacgg ccgcaccggc ttccagaacc tcatcgcctg cgtcgagctg
     4081 agcgagaagg aagccgccct gatggaccag ggcaaccacg gctcccacca cgcctcgaag
     4141 aagagcaagc tccaggtcaa ggcgcagctg tccaacccgg gcctgcgcga cgacgccgac
     4201 ctcgccgccc gcgtcgccta cgacctgccg ggcgccgagc cgacccccga gcagcgcagc
     4261 cgggtcttcg cccgcaagac gtaccgcttc tacgagggcg gcgcggtcac cgaggccgac
     4321 ctgctggcgc tcctcggcgg ccaggtcccc gcggcctact cgcgcaaggc cgccgacctg
     4381 gcgccggccg aactgggcca gatcctgcgc tggttcggcc agtacctcag cgaggagcgg
     4441 ctgctgccca agtacggcta cgcctccccg ggcgcgctgt acgcgacgca gctgtacttc
     4501 gagctggagg gcgtcggcgg gctgcagccg ggctactact actaccagcc gcagcgccac
     4561 cagctggtcc tgatcagcga gaaggccgcc accggccggc ccaccgccca catccacttc
     4621 atcggcaagc gcggcggcat cgagccggtc tacaagaaca acatccagga ggtcctggag
     4681 atcgagaccg gccacatcgt cggcctcttc gagcaggtgc tgccggcgta cggcctcgac
     4741 atccgggacc tggcctacga gccggccgtc cgcgacctgc tggacgtgcc cgaggaggac
     4801 ttctacctcg gcaccttcga gctggtcccg cacacgggcc ggcgcgagga ccacgcggag
     4861 gtctacgtcc agacccacgg cagcaaggtc gcgaacctcc ccgagggcca gtaccgctac
     4921 gcggacggca cgctcacccg cttctcggac gacatcgtcc tcaagaagca ggtcatcgcg
     4981 atcaaccagt cggtgtacca ggccgccagc ttcggcatct cggtgatcag ccgtgccccc
     5041 gaggagtgga tgcactacgt caccctcggc aagaagctcc agcacctgat gatgaacggc
     5101 ctcggcctcg gcttcatgtc ctcgggctac agctcgaaga ccggcaaccc gctgccggcc
     5161 tcccgccgga tcgactccgt cctgcaggcg aacggcgtcg agagcggtcc gtcgtacttc
     5221 ttcgtcggcg gccgcgtcag cgacgagcag ctcggccacg agggcatgcg cgaggacagc
     5281 gtccacatgc gcggtccggc ggagctgatc cgtgacgacc tcgtcagctt cctgccggac
     5341 tacatgatcc cgaaccgggt cgtggtgttc gagcggctgc cgctgtccgc caacggcaag
     5401 atcgacgcga aggcgctggc cgcctccgac caggtcaacg ccgagctcgt ggagcggccc
     5461 ttcgtcgccc cgcgcaccga gaccgagaag gagatcgcgg aggtctgggc caagtccctg
     5521 cgccgcgaga gcgtctcggt ccaggacgac ttcttcgagt cgggcggcaa ctcgctgatc
     5581 gccgtcggcc tcatccgcga gctcaactcg cgcctgggcg tctccctgcc gctgcagtcc
     5641 gtcctggagt cccccacggt cgagaagctc tcccgccgcc tggagcgcga ggtcgcgcag
     5701 gagtcctccc ggctggtgcg cctgcacgcg gagaccggca aggaccgtcc ggtgctgtgc
     5761 tggccgggcc tgggcggcta cccgatgaac ctgcgcaccc tggccggcga gatcggcctc
     5821 ggccgctcgt tctacggcat ccaggcgcac gggatcaacg agggcgaggc cccgtacgcg
     5881 accatcacgg agatggccaa ggccgacatc gaggccatca aggagctcca gccgaagggc
     5941 ccctacaccc tgtggggcta ctccttcggc gcccgcgtcg ccttcgagac cgcctaccag
     6001 ctggagcagg cgggcgagaa ggtggacaac ctcttcctga tcgccccggg ctcgccgacc
     6061 gtgcgcgccg agaacggcaa ggtgtacggc cgtgaggcgt ccttcgccaa ccgcgcctac
     6121 acgaccatcc tcttctcggt gttcaccggc accatttcgg gtccggacct ggaaaagtgc
     6181 ctggaatccg ccacggacga ggagtccttc gccgggttca tcagcgaact gaagggaatc
     6241 gacgtcgatc tggcgaagcg gatcatctcg gtcgtggggc agacctacga gttcgagtac
     6301 tcgttccgtg agctggccga gcgcaccctg gccgcgccgg tgaccatatt caaggcccgc
     6361 ggcgacgact actcgttcat cgagaacagc aacggttact ccgccgagcc cccgacggtc
     6421 atcgacctcg acgccgacca ctacagcctg ctccgcaccc cggacatcgg cgagctggtc
     6481 aagcacatcc ggtacctgct cggagagtag gggaaagccg gtcacagaac acggccgccc
     6541 cgtcggagaa atccggcggg gcggccgtgt ttttgcattt caggacctgt cggaattcgt
     6601 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7201 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7441 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7681 aaaaaggatc ttcacctaga tccttttggt tcatgtgcag ctccatcagc aaaaggggat
     7741 gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg
     7801 atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc
     7861 ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc
     7921 cgtagcgtcc ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctgcg
     7981 ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc
     8041 gatcctgcca cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc
     8101 ctgccaaatg taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca
     8161 gagcagatca tctctgatcc attgcccctg ccacctcact cgcctgcaag cccggtcgcc
     8221 cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg
     8281 acgctgcatc ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc
     8341 attcttcagg atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag
     8401 cgctttgcct tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc
     8461 ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa
     8521 ctgggccgag atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat
     8581 gccgctcgcc agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc
     8641 aaggtcgcgc tgattgctgg ggcaacacgt ggagcggatc ggggattgtc tttcttcagc
     8701 tcgctgatga tatgctgacg ctcaatgccg tttggcctcc gactaacgaa aatcccgcat
     8761 ttggacggct gatccgattg gcacggcgga cggcgaatgg cggagcagac gctcgtccgg
     8821 gggcaatgag atatgaaaaa gcctgaactc accgcgacgt atcgggccct ggccagctag
     8881 ctagagtcga cctgcaggtc cccggggatc ggtcttgcct tgctcgtcgg tgatgtactt
     8941 caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac
     9001 gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca
     9061 cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga
     9121 ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca
     9181 ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt
     9241 ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca
     9301 tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
     9361 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
     9421 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     9481 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     9541 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     9601 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
     9661 agcagggtta tgcagcggaa aagatccgtc gacctgcagg catgcaagct ctagcgattc
     9721 cagacgtccc gaaggcgtgg cgcggcttcc ccgtgccgga gcaatcgccc tgggtgggtt
     9781 acacgacgcc cctctatggc ccgtactgac ggacacaccg aagccccggc ggcaaccctc
     9841 agcggatgcc ccggggcttc acgttttccc aggtcagaag cggttttcgg gagtagt
//
