LOCUS       40924_48253       12563 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYPX4062, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Yao Q, Peng R, Xiong A.
  TITLE     Broad-host-range expression vector pYPX4062 which can express two 
            different genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   Yao Q, Peng R, Xiong A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, Beidi Rd 2901, Shanghai 201106, 
            China
REFERENCE   3  (bases 1 to 12563)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12563)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, Beidi Rd 2901, Shanghai 201106, 
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      23..275
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    297..321
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1621..2247
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2684..3748
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3817..4011
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4355..4495
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4681..5269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5359..6150)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     terminator      complement(6359..6386)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6478..6564)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(6775..7305)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(7496..7524)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        7688..7759
                     /label=AmpR promoter
     CDS             7760..8617
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             9056..12127
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     terminator      12344..12430
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      12522..12549
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
       61 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
      121 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
      181 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
      241 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc agtgtttgac
      301 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta
      361 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
      421 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
      481 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
      541 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
      601 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
      661 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
      721 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
      781 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
      841 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
      901 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
      961 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     1021 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     1081 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     1141 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     1201 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     1261 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     1321 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     1381 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     1441 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     1501 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     1561 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     1621 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     1681 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     1741 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     1801 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     1861 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     1921 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     1981 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     2041 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     2101 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     2161 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     2221 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     2281 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     2341 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     2401 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     2461 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     2521 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     2581 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc
     2641 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca aaccatccgg
     2701 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     2761 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     2821 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     2881 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     2941 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     3001 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     3061 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     3121 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     3181 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     3241 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     3301 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     3361 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     3421 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     3481 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     3541 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     3601 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     3661 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     3721 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     3781 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa
     3841 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     3901 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     3961 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     4021 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     4081 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     4141 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     4201 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     4381 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4441 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     4501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4921 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4981 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5041 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5101 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5161 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5221 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5281 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5341 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
     5401 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
     5461 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
     5521 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
     5581 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
     5641 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
     5701 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
     5761 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
     5821 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
     5881 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
     5941 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
     6001 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
     6061 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
     6121 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
     6181 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
     6241 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
     6301 aacatagtat cgacggagcc gattttgaaa ccgcgggcgg ccgctttgta gaaacgcaaa
     6361 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
     6421 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
     6481 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     6541 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagacc
     6601 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
     6661 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
     6721 taatctgtat caggctgaaa atcttctctc atccgccaaa acagcgagct cttaggtggc
     6781 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     6841 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     6901 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     6961 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag
     7021 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     7081 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     7141 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     7201 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     7261 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatggatc cgctgctcca
     7321 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     7381 ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     7441 gcgttcggtc aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt
     7501 acgaaccgaa caggcttatg tcaactgggt tcgtgccttc atccgtttcc acgcgtgcgg
     7561 ccgctgcagc tcgagggaat gtctgtcaga gatctattct gccgacatgg aagccatcac
     7621 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     7681 atttgcccat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7741 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7801 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     7861 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     7921 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     7981 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8041 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8101 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8161 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8221 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8281 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8341 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8401 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8461 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8521 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8581 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8641 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8701 cctttttgat aatctcatga ccaaaatcca ggtggcactt ttcggggaaa tgtgcgcgcc
     8761 cgcgttaagc ttagatctct cacctaccaa acaatgcccc cctgcaaaaa ataaattcat
     8821 ataaaaaaca tacagataac catctgcggt gataaattat ctctggcggt gttgacataa
     8881 ataccactgg cggtgatact gagcacatca gcaggacgca ctgaccacca tgaaggtgac
     8941 gctcttaaaa attaagccct gaagaagggc agcattcaaa gcagaaggct ttggggtgtg
     9001 tgatacgaaa cgaagcattg gttaaaaatt aaggaggggg ggcccccccg aattcatgac
     9061 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9121 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9181 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
     9241 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
     9301 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
     9361 caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg
     9421 ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat
     9481 tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
     9541 cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga
     9601 aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc agttatctgg aagatcagga
     9661 tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgactacaca
     9721 aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg ctgtactgga
     9781 ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag tttctttatg
     9841 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
     9901 gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc cgaaactgtg
     9961 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac
    10021 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
    10081 gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg agcatcatcc
    10141 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
    10201 gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct
    10261 gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt
    10321 gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac
    10381 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
    10441 atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc
    10501 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
    10561 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
    10621 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
    10681 gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc agtatccccg
    10741 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
    10801 cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt
    10861 ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cggaagcaaa
    10921 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
    10981 atacctgttc cgtcatagcg ataacgaact cctgcactgg atggtggcgc tggatggtaa
    11041 gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac agttgattga
    11101 actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag tacgcgtagt
    11161 gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc agcagtggcg
    11221 tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc cgcatctgac
    11281 caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca
    11341 gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga cgccgctgcg
    11401 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
    11461 tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
    11521 gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga ccgctcacgc
    11581 gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
    11641 tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc atccggcgcg
    11701 gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc tcggattagg
    11761 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
    11821 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
    11881 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg
    11941 ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
    12001 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
    12061 gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg
    12121 tcaaaaataa taataaggta ccgctgtttt ggcggatgag agaagatttt cagcctgata
    12181 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
    12241 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
    12301 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
    12361 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag
    12421 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg
    12481 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga
    12541 tggccttttt gcgtttctac aaa
//
