LOCUS       62056_23170       14254 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             192..218
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             309..1019
                     /codon_start=1
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     protein_bind    2314..2437
                     /label=attR5
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2455..2473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2478..2494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2925..3713
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3809..4397
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4727..4754)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4846..4932)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    5028..5127
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5266..9366
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9592..9645
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9649..10035
                     /codon_start=1
                     /label=MS2-N55K
                     /note="bacteriophage MS2 coat protein"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             10036..10056
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             10099..10125
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             10144..10164
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             10171..10191
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             10255..10311
                     /codon_start=1
                     /label=GCN4_v4
                     /note="GCN4 peptide optimized to improve solubility while 
                     preserving binding to an scFv-GFP fusion protein (Tanenbaum
                     et al., 2014)"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11041..11061
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     promoter        11701..13698
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
ORIGIN
        1 ttatctgggg cgacggaata acagattata actctgcttt gaaggacaga ttcatcatta
       61 gcaaggacaa cgggaagaac acggtgtacc tccagatgag caaagtgcgc agtgatgata
      121 ctgccctgta ttactgcgtc acaggtttgt ttgattactg gggtcaaggc actcttgtga
      181 cagtctcttc gtatccttat gacgtgcccg actacgctgg cggagggggc ggcagcggag
      241 gcggtggatc cggaggcggt ggctcagggg gcggcgggtc gcttgatcct ggtggcggag
      301 gttctgggag caaaggagag gagctcttta caggtgtcgt ccctattttg gtggagcttg
      361 atggagatgt caatggccat aaattttccg tcaggggcga aggggagggg gacgcgacta
      421 atggaaaatt gaccctcaag tttatctgca cgacaggaaa gttgccggtc ccctggccca
      481 ctctggttac caccctcact tatggggtcc agtgctttag cagataccct gatcatatga
      541 aacgccatga cttcttcaaa tcagccatgc cagagggtta tgtgcaggaa agaactataa
      601 gctttaagga cgatgggacg tacaaaacgc gggcagaggt taagtttgag ggggatactc
      661 ttgtgaatag gatcgaactt aagggcatcg attttaaaga agacggtaat attcttggcc
      721 acaagttgga gtataatttc aattctcaca acgtgtacat aacagcagat aaacagaaaa
      781 atgggatcaa ggccaatttt aaaattaggc ataatgttga agatgggtct gtgcaactgg
      841 cagatcatta tcagcagaat actccaattg gcgatggccc tgtcttgttg cctgacaatc
      901 attacctttc cacgcagtca gtcctgtcca aagatccgaa tgagaagcgg gaccacatgg
      961 tcttgctcga attcgttact gccgccggaa tcactcatgg catggacgag ctttacaagg
     1021 gaggtggaag gactggaggt ggcggagggg ggctgcttga ccccggaacc cctatggatg
     1081 ccgacttggt ggcctcctcc acggttgttt gggaacagga tgccgaccct tttgcgggca
     1141 ctgctgacga ttttcccgct ttcaatgagg aggagttggc ttggctgatg gaacttctcc
     1201 cacagggtgg tagtggtggc ctcctggatc cggggacacc gatggacgca gacctggtgg
     1261 cgtcgtcaac ggttgtgtgg gaacaggatg ctgatccgtt cgcaggaact gccgacgatt
     1321 ttcctgcatt caacgaagag gaactcgcat ggctcatgga gcttctgcct cagggatcgg
     1381 gtggcgggtc tcgaacggaa gaatacaagc ttatattgaa cggtaagaca ctgaaaggtg
     1441 aaactacgac agaagccgtt gacgcggcaa ctgcggagaa agtctttaag cagtatgcta
     1501 acgataatgg cgtggacggg gagtggacgt atgacgacgc aaccaaaaca ttcacggtca
     1561 ccgagggggg cggcagcgga ggcggtacct cgcccaaaac ccggagacgc cctaggcgct
     1621 cgcagcgcaa aagacctccc acgtaaagag ctttcgttcg tatcatcggt ttcgacaacg
     1681 ttcgtcaagt tcaatgcatc agtttcattg cgcacacacc agaatcctac tgagtttgag
     1741 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
     1801 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
     1861 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
     1921 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
     1981 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgta
     2041 ccattatgct tattcactag gcaacaaata tattttcaga cctagaaaag ctgcaaatgt
     2101 tactgaatac aagtatgtcc tcttgtgttt tagacattta tgaactttcc tttatgtaat
     2161 tttccagaat ccttgtcaga ttctaatcat tgctttataa ttatagttat actcatggat
     2221 ttgtagttga gtatgaaaat attttttaat gcattttatg acttgccaat tgttccggaa
     2281 ctctagataa gcttaccgga aagggcgaat tcgcaacttt gtatacaaaa gttgaacgag
     2341 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     2401 cataatactg taaaacacaa catatccagt cactatgcca tccagctgat atcccctata
     2461 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2521 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctgg accagccagg
     2581 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2641 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2701 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2761 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2821 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2881 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2941 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     3001 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     3061 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     3121 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     3181 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3241 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3301 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3361 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3421 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3481 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3541 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3601 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3661 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3721 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3781 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3841 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3901 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4321 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4381 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4441 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4501 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4561 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4621 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4681 caattaatac gcgtaccgcg agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     4741 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4801 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4861 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4921 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgcttgca tggatgtttt
     4981 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     5041 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
     5101 tgccaacttt gtacaaaaaa gcaggctccg aattcgccct tcaccatgga ttacaaggac
     5161 cacgacgggg attacaagga ccacgacatt gattacaagg atgatgatga caagatggct
     5221 ccgaagaaga agaggaaggt tggcatccac ggggtgccag ctgctgacaa gaagtactcg
     5281 atcggcctcg ctattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag
     5341 gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat
     5401 ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc
     5461 accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc
     5521 tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc
     5581 gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt
     5641 gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca
     5701 gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc
     5761 catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc
     5821 cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc
     5881 gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc
     5941 gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg
     6001 gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc
     6061 tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac
     6121 gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc
     6181 agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac
     6241 gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag
     6301 tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg
     6361 gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg
     6421 gaggagctgc tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat
     6481 aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag
     6541 gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc
     6601 aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc
     6661 cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct
     6721 agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag
     6781 gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag
     6841 gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag
     6901 gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag
     6961 gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc
     7021 ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc
     7081 ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc
     7141 gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag
     7201 gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc
     7261 atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat
     7321 ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag
     7381 gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac
     7441 ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag
     7501 ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag
     7561 aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag
     7621 ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg
     7681 cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag
     7741 gagctggata ttaacaggct cagcgattac gacgtcgatg ctatcgttcc acagtcattc
     7801 ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag
     7861 tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc
     7921 ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc
     7981 gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag
     8041 atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat
     8101 gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc
     8161 aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac
     8221 gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc
     8281 gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag
     8341 caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc
     8401 aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac
     8461 ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt
     8521 ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct
     8581 aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg
     8641 gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg
     8701 gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc
     8761 acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc
     8821 tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg
     8881 gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg
     8941 gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag
     9001 ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc
     9061 gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat
     9121 ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct
     9181 gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac
     9241 ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc
     9301 ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg
     9361 ggcgacaagc ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaagaaggga
     9421 gacggctctg gatcggggtc gggttctggc tcagtcgacg atgctcttga cgattttgac
     9481 ctcgatatgc tcgacgctct tgatgatttt gatctcgaca tgctcgatgc acttgatgac
     9541 tttgaccttg acatgctcga cgcactcgat gacttcgacc tcgacatgct tgagggcaga
     9601 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatggc gtcaaatttc
     9661 acgcagtttg ttttggttga taacggcggg actggcgacg ttacagtagc tccatcaaat
     9721 tttgcgaacg gagtcgctga gtggattagc tcaaattcaa ggtcccaggc ctacaaggtt
     9781 acctgttctg ttaggcagag ttctgcgcaa aaaagaaaat ataccatcaa ggttgaagtc
     9841 cctaaagttg caacacaaac agtcggtggt gttgagctcc ctgtggcagc ctggagatct
     9901 tacttaaaca tggagctaac aattccaata ttcgctacaa actctgattg tgaactgatt
     9961 gttaaggcga tgcaaggtct cttgaaagat ggaaacccta taccgtccgc tatcgcagct
    10021 aacagcggta tctatcctaa gaagaagagg aaggttggct ctggatcggg gtcgggttct
    10081 ggctcaggat ccggtaccta tccctatgac gtgcccgatt atgccagcct gggcagcggc
    10141 tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt ggacggcatt
    10201 ggtagtggga gcaacggcag cagcggatcc aacggtccga ctgacgccgc ggaagaagaa
    10261 cttttgagca agaattatca tcttgagaac gaagtggctc gtcttaagaa aggttctggc
    10321 agtggagaag aactgctttc aaagaattac cacctggaaa atgaggtagc tagactgaaa
    10381 aaggggagcg gaagtgggga ggagttgctg agcaaaaatt atcatttgga gaacgaagta
    10441 gcacgactaa agaaagggtc cggatcgggt gaggagttac tctcgaaaaa ttatcatctc
    10501 gaaaacgaag tggctcggct aaaaaagggc agtggttctg gagaagagct attatctaaa
    10561 aactaccacc tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgaagag
    10621 ctactatcca agaattatca tcttgagaac gaggtagcgc gtttgaagaa gggttccggc
    10681 tcaggagagg aactgctctc gaagaactat catcttgaaa atgaggtcgc tcgattaaaa
    10741 aagggatcgg gcagtggtga ggaactactt tcaaagaatt accacctcga aaacgaagta
    10801 gctcgattaa agaaaggttc agggtcgggt gaagaattac tgagtaaaaa ttatcatctg
    10861 gaaaatgagg tagcgagact aaaaaagggg agtggttctg gcgaggaatt gctatcgaaa
    10921 aattatcatc ttgagaacga agttgctagg ctcaaaaagg gctcaggctc aggcaccgcg
    10981 gtaaacatag gtggtggaac cggtccgatg gatctacagc ggccgcaagg tggaggtgga
    11041 cccaagaaga agcgcaaggt gtgaactagt taaagagctt tcgttcgtat catcggtttc
    11101 gacaacgttc gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga
    11161 gtttgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
    11221 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
    11281 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
    11341 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
    11401 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt
    11461 agttgtacca ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg
    11521 caaatgttac tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt
    11581 atgtaatttt ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact
    11641 catggatttg tagttgagta tgaaaatatt ttttaatgca ttttatgact tgcccgtacg
    11701 ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta
    11761 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta
    11821 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa
    11881 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga
    11941 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt
    12001 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg
    12061 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt
    12121 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata
    12181 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa
    12241 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga
    12301 cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga
    12361 cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg
    12421 acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac
    12481 ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc
    12541 gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct
    12601 ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca
    12661 cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc ccctctctac
    12721 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
    12781 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
    12841 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
    12901 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
    12961 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
    13021 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
    13081 ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat
    13141 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
    13201 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
    13261 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
    13321 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
    13381 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
    13441 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
    13501 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
    13561 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
    13621 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
    13681 ttggtgttac ttctgcaggg cgccatgggg ccggacatag tcatgacaca aagtccgagt
    13741 tcgctttctg cgagtgttgg ggatagagtt acgataacct gcagatctag tacgggggcc
    13801 gtcaccacgt cgaattacgc gtcatgggtc caagaaaagc caggcaaact gtttaagggt
    13861 cttataggtg gcacgaacaa ccgggctccc ggagtccctt ccaggtttag tggatcactt
    13921 attggtgaca aggcaacgct gacgatatct tccctccagc cagaagattt cgcgacctac
    13981 ttttgtgcac tttggtattc taaccactgg gtctttggcc aagggacgaa ggtcgagctc
    14041 aaacggggtg gcggtgggag cggcggggga ggatcagggg gcggaggttc gagtggcggg
    14101 gggagcgaag tcaaactcct cgagtccggt ggaggattgg tgcaaccagg tggttcattg
    14161 aagctctcgt gtgcagtttc tggtttctct ttgacagact acggtgtcaa ttgggtccgg
    14221 caggcaccgg ggcgcggtct tgagtggatc ggcg
//
