LOCUS       40924_48213        7331 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pYM70, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Basso LR Jr., Gast CE, Mao Y, Wong B.
  TITLE     Fluconazole transport into Candida albicans secretory vesicles by 
            the membrane proteins Cdr1p, Cdr2p, and Mdr1p
  JOURNAL   Eukaryotic Cell 9 (6), 960-970 (2010)
  PUBMED    20348384
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Basso LR Jr., Bartiss A, Mao Y, Gast CE, Coelho PS, Snyder M, Wong 
            B.
  TITLE     Transformation of Candida albicans with a synthetic hygromycin B 
            resistance gene
  JOURNAL   Yeast 27 (12), 1039-1048 (2010)
  PUBMED    20737428
REFERENCE   3  (bases 1 to 7331)
  AUTHORS   Mao Y, Basso LR Jr., Gast CE, Wong B.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-FEB-2010) Medicine, Oregon Health and Science 
            University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
REFERENCE   4  (bases 1 to 7331)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7331)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2010"; volume: "9"; issue: "6"; pages: "960-970"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Yeast"; 
            date: "2010"; volume: "27"; issue: "12"; pages: "1039-1048"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (24-FEB-2010) Medicine, Oregon Health and Science University, 3181 
            SW Sam Jackson Park Road, Portland, OR 97239, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      189..1390
                     /label=Candida albicans autonomous replication origin 2
                     /note="Candida albicans autonomous replication origin 2"
     primer_bind     1587..1603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1637..2652
                     /label=Candida albicans ACT1 promoter
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     regulatory      2684..3009
                     /label=Candida albicans TEF2 terminator
                     /note="Candida albicans TEF2 terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3037..3053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3061..3077)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3085..3115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3130..3151)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3439..4027)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4201..5058)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5059..5163)
                     /label=AmpR promoter
     regulatory      5195..5835
                     /label=Candida albicans TEF2 promoter
                     /note="Candida albicans TEF2 promoter"
                     /regulatory_class="promoter"
     CDS             5842..6864
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     regulatory      6874..7261
                     /label=Candida albicans ACT1 terminator
                     /note="Candida albicans ACT1 terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgga tcataccaaa aaaaaatccg gggtagcgat gaggtagtgc aagttatacc
      241 agagagcctc aatttgaacg tggtactgca cacaaaccgt agccctaacc ctaattaaat
      301 gcacgtgacc cacacaattt tcaaccacca acaacacacc aaaaatgtat gtacactcgg
      361 agtggaaaat atttccccag gcataattgt ggttgccaat tatagtggag tgtttgttat
      421 tagtatagga gtactccttt tatgtggaaa aatcgaaaaa cataaaacag tgcagctata
      481 cactaacaaa tggtataccc atggtcagac aacaccaaac aacacacccg taatggttgg
      541 tttggctaaa tagagcatct cattttgtgt attattttat gttttggata tttttagttt
      601 gaaatttata taaaaatatt ttacttcaat tttcttgcca aattttgtac aaaaagtaaa
      661 aaatagaact tccaattttt gttagacaaa cttcaatcta acaaccggga tagtgttttg
      721 ggggctgtgt aacaccactg gtgtaataaa agtgccaata tttggaaatt aattttttgg
      781 tggtggacgt ttcttcctaa aacacctaga tgtgctagta taattgcaca accagcacgc
      841 acactgcgtc tggccggccc tggactacat tttgtctcta acattcgtag ctgcagcact
      901 gctacaaccc agtcaactgg ccacccggac atacatattc ccgttttgtg ccacagccac
      961 ccaactttcc ggctcaaaaa atggctccac acaatttggg caccccgatt acccctcccc
     1021 tgcagcgaca gcacatacac tttggtggtt gtggggctag agccctaaaa gtgaatttgg
     1081 tcacgtgaga gacagcagat aaggttcgca tttttttatt ttttcggttg cggccatatc
     1141 tagcagaaag caccgttccc cgttcgatca accgtagtta agctgctaag agcaataccg
     1201 agtagtgtag tgggagacca tacgcgaaac tattgtgctg caatctattt tttttagtaa
     1261 ggtatatttt ttttttgcat gaaccaaggg taatcgaccc tgctctatcc agtgatggtg
     1321 ataaagttgg gtggtgtcta acaaaaaggg ggagaaccag agggtgtata aataaggaga
     1381 agctgggatc catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     1441 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     1501 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     1561 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct
     1621 gcaggtcgac tctagaagag ctattaagat caccagcctc gtttataata aacttagtct
     1681 aatatttctt ttggaccaaa atagaaactg ttctcaggtg gcaaaaaaca gcttaaagtc
     1741 tgtgttaaaa aaagatctcg gctatgccaa tcaaaaagga gtgacattat tatgtaaaag
     1801 aagaaatgtt gtaattccgc gaaacaacaa ttttaatgct tactgtgccg ccaccaatat
     1861 cctatggcca aggggccttt aaacatgcca cggaaatata cagtcaaagt aaacctacta
     1921 tgtctttaga gccttcagga tcaattgttt ttaaatgaat gactgcaaaa ctagaaaagt
     1981 agcctcttac ataagcaagt catttgtaca ccgagagttt agtaaccagt gaagcaagta
     2041 tatctggcaa acctgaaact tatctgtgaa ttataaagct cataagtaaa aagtgtagaa
     2101 aagaagataa ggcagaccaa atacaaattt gcaacagtac aacgaatatt tcagcgtcaa
     2161 aactagagaa taataaagaa aacgatcttt tcaaaaagaa aaaacctttt agttttcctt
     2221 tgttgttgtt gtgggtgtgt gctatttata ttatatagtt tactcataat accataaaat
     2281 attcggtttg attaggttat tttaataagc taatttgttt ctaatcgtgt aatttatgct
     2341 gtgtatatta agtagtgtgt gcactgccca aaaatgtttg ttgtttatag tcggttaaag
     2401 agaaaaaaga aaaaaagatc catacacaca cgttaattag ttgttcaacg taatacactc
     2461 atattttgtt cttatttgct ttcggtcgct gttctcacca agatttattg ccaacgaaac
     2521 aatttttttt tatatatttt cagatttttc tttttttcct ttcctttcct tttctaattt
     2581 tcactcctgg ttttctttct ttcttagaaa cattatctcg atattaatat taaaaaaata
     2641 taatcattca aattaattaa ccgcggacta gtggatcccc gggtaaacta attaaatacc
     2701 tttgtttaaa tagttgtgat tatattattt ttttaaaata tatataaata agagaataat
     2761 catacaacat gtagtaagga gcgaagagtc ccagctctgt aaataaaaaa aagaagagga
     2821 gttatgtgct tctttgttta tgtatatata tatattctta ccagttagtt aatcttacac
     2881 atatgttgat ggtgctatat tgtacataca aactctaaat catatcagaa ctgcttggaa
     2941 acctttccca ctaacaacac ccatccaatc cccttggttc tgtctccaac acacgttatt
     3001 gatttccaag tcgaggagct cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     3061 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3121 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3181 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3241 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     3301 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     3361 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     3421 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     3481 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     3541 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     3601 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     3661 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3721 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3781 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3841 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     3901 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3961 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4021 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4081 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4141 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4201 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4261 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4321 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     4381 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     4441 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     4501 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     4561 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     4621 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     4681 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4741 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4801 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4861 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4921 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4981 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5041 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5101 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5161 gcgcacattt ccccgaaaag tgccacctga cgtcgtatag tgcttgctgt tcgatattgc
     5221 tagaggcaaa gaaaaaggat tacctcacgc ctaaaatgga atttgaaaat tgaggagatg
     5281 agttgagctt ttgtctttta cactttccct attcaattgt gccgcaaaaa cttcaatatc
     5341 taacacagtg tgaaatattt gttatgtata ccgaaagcca aataccattt aaactggtat
     5401 ttcaagattt cataaatcaa gtttctggtt aaattaattg ctgttaaaca gaacaagagc
     5461 ttcacattga tgttggaata actactatag aattataaaa tacacaaaac caggtcgtgt
     5521 acgcaataac ttttcaatgt atgaaaaaat tttttttttt tttttgcttc ttctcttcgc
     5581 ttcttttttc tagttatcaa tttggcaatg aattgatttg attgcccttt gtttagttta
     5641 ctatgaaaaa aaattttttt tttgtctctg tcagattttc tctcattttt ttttttgcct
     5701 cataatgaac aactcaaaaa agaatataaa tttctcatca aagacctttc caattgaaaa
     5761 ttttttcttt ccttcttttc cttttttttt ttctttcttt cttcttttct aagtatacat
     5821 tttatataat caatcctcga gatgaaaaaa ccagaattga ctgctacttc tgttgaaaaa
     5881 tttttgattg aaaaatttga ttctgtttct gatttgatgc aattgtctga aggtgaagaa
     5941 tcaagagctt tttcttttga tgttggtggt agaggttatg ttttgagagt taattcttgt
     6001 gctgatggtt tttataaaga tagatatgtt tatagacatt ttgcttctgc tgctttgcca
     6061 attccagaag ttttggatat tggtgaattt tctgaatctt tgacttattg tatttcaaga
     6121 agagctcaag gtgttacttt gcaagatttg ccagaaactg aattgccagc tgttttgcaa
     6181 ccagttgctg aagctatgga tgctattgct gctgctgatt tgtctcaaac ttctggtttt
     6241 ggtccatttg gtccacaagg tattggtcaa tatactactt ggagagattt tatttgtgct
     6301 attgctgatc cacatgttta tcattggcaa actgttatgg atgatactgt ttctgcttct
     6361 gttgctcaag cattggatga attgatgttg tgggctgaag attgtccaga agttagacat
     6421 ttggttcatg ctgattttgg ttctaataat gttttgactg ataatggtag aattactgct
     6481 gttattgatt ggtctgaagc tatgtttggt gattctcaat atgaagttgc taatattttt
     6541 ttttggagac catggttggc ttgtatggaa caacaaacta gatattttga aagaagacat
     6601 ccagaattgg ctggttctcc aagattgaga gcttatatgt tgagaattgg tttggatcaa
     6661 ttgtatcaat ctttggttga tggtaatttt gatgatgctg cttgggctca aggtagatgt
     6721 gatgctattg ttagatcagg tgctggtact gttggtagaa ctcaaattgc tagaagatca
     6781 gctgctgttt ggactgatgg ttgtgttgaa gttttggctg attctggtaa tagaagacca
     6841 tctactagac caagagctaa agaataagga tccgagtgaa attctggaaa tctggaaatc
     6901 tggttttgta ttcttgttat tcttcttttt gttattacat atataacttg ttactttttt
     6961 aaaaaaatct ttgtttattt tataaatata taaaactaaa tttaagaaaa agagaaaaat
     7021 gttttatttg agagattgaa attttacttg aatttagctt agcttttata aagtattatt
     7081 atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga ctataaaata gccacccaag
     7141 gcatttctat atcttgttgt tgttgttttc atcttctgta tcagaggaac ttattttatt
     7201 attttcgtca cgggtatttt ctcttgtttg atgattcatc ccattcattc catcataaaa
     7261 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7321 gccctttcgt c
//
