LOCUS       Exported                4866 bp DNA     circular SYN 22-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   P30247
VERSION     .
KEYWORDS    pYM35
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Self
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 22, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished."
COMMENT     A versatile toolbox for PCR-based tagging of yeast genes: new 
            fluorescent proteins, more markers and promoter substitution 
            cassettes. Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, 
            Maekawa H, Moreno-Borchart A, Doenges G, Schwob E, Schiebel E,
            Knop M. Yeast (2004) 21, 947 - 962
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..759
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     terminator      781..968
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     gene            1043..2399
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1043..1386
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1387..2196
                     /gene="kanMX"
                     /label=kanMX
     terminator      2202..2399
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(2504..2522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2780..3368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3539..4399)
                     /gene="AmpR"
                     /label=AmpR
     promoter        complement(4400..4504)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4850..4866,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
       61 ggtgctggtg ctggagcaat ggtgcgctcc tccaagaacg tcatcaagga gttcatgcgc
      121 ttcaaggtgc gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc
      181 gagggccgcc cctacgaggg ccacaacacc gtgaagctga aggtgaccaa gggcggcccc
      241 ctgcccttcg cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg
      301 aagcaccccg ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg
      361 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccaaga ctcctccctg
      421 caggacggct gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc
      481 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
      541 gacggcgtgc tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac
      601 ctggtggagt tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac
      661 tacgtggact ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag
      721 tacgagcgca ccgagggccg ccaccacctg ttcctgtagc tgcgcgccac ttctaaataa
      781 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
      841 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
      901 ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc
      961 tctaccggca gatccgctag ggataacagg gtaatataga tctgtttagc ttgcctcgtc
     1021 cccgccgggt cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga
     1081 cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca
     1141 tgtataatca tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg
     1201 ctcctcgctg cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc
     1261 ccacgccgcg cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt
     1321 tcatatactt ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa
     1381 acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat
     1441 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1501 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1561 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1621 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1681 atccccggca aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1741 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1801 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1861 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     1921 gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     1981 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2041 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2101 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2161 ttgcagtttc atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc
     2221 ttgttttcaa gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat
     2281 caaatgttag cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt
     2341 taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc
     2401 tgtcgattcg atactaacgc cgccatccag tttaaacgag ctcgaattca tcgatgatat
     2461 cagatccact agtggcctat gcggccgcgg atctgccggt ctccctatag tgagtcgtat
     2521 taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2581 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2641 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2701 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2761 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2821 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2881 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2941 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     3001 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3061 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3121 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3181 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3241 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3301 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3361 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3421 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3481 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3541 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3601 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3661 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3721 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3781 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3841 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3901 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3961 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4021 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4081 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4141 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4201 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4261 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4321 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4381 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4441 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4501 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     4561 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     4621 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     4681 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     4741 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatggac atattgtcgt
     4801 tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac
     4861 actata
//
