LOCUS       pYM26.        4298 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Plasmid with a TRP1 marker that provides a template for PCR to fuse 
            yeast enhanced GFP (yeGFP) to the C-terminus of a protein of 
            interest.
ACCESSION   .
VERSION     .
KEYWORDS    pYM26
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Janke C, Magiera MM, Rathfelder N, Taxis C, Reber S, Maekawa H, 
            Moreno-Borchart A, Doenges G, Schwob E, Schiebel E, Knop M.
  TITLE     A versatile toolbox for PCR-based tagging of yeast genes: new 
            fluorescent proteins, more markers and promoter substitution 
            cassettes.
  JOURNAL   Yeast 2004;21:947-62.
  PUBMED    15334558
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   EUROSCARF
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4298)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004"; volume: "21"; pages: "947-62"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             82..795
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     CDS             complement(886..1518)
                     /codon_start=1
                     /gene="Kluyveromyces lactis TRP1"
                     /product="phosphoribosylanthranilate isomerase, required
                     for tryptophan biosynthesis"
                     /label=Kluyveromyces lactis TRP1
                     /note="KlTRP1"
                     /note="K. lactis auxotrophic marker that also works in S. 
                     cerevisiae"
                     /translation="MLVKVCGLQTVEAAKTAVDDGADYLGIICVPGRKRTIDSSVAKGI
                     STAVHQQENVKGTKLVGVFRNQSVDDVLQLYHEYNLDVIQLHGDEDIKEYRSLIPSSIP
                     IIKRFQFPQDCELLLDLYEHVDNVLTLFDSGEGGTGEKLNWSAISSWSASHPEIKFIIA
                     GGLNPDNVSVAINMLPNAIGVDVSGGVETDGIKDLEKVKLFIQQASQ"
     promoter        complement(1936..1954)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(2212..2800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2974..3831)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3832..3936)
                     /label=AmpR promoter
     promoter        4282..4298
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
       61 ggtgctggtg ctggagcaat tctgtctaaa ggtgaagaat tattcactgg tgttgtccca
      121 attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt
      181 gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg
      241 ccagttccat ggccaacctt agtcactact ttcggttatg gtgttcaatg ttttgcgaga
      301 tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt
      361 caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag
      421 tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt taaagaagat
      481 ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt ttacatcatg
      541 gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc
      661 ttgttaccag acaaccatta cttatccact caatctgcct tatccaaaga tccaaacgaa
      721 aagacagacc acatggtctt gttagaattt gttactgctg ctggtattac ccatggtatg
      781 gatgaattgt acaaataact gcgcgcaaag tggaacgatc attcactata tatatatcaa
      841 tttatatata cgtatgtgta attgaagaaa gatacgtttt ttcctctatt gagaggcctg
      901 ctggatgaat agctttacct tttctaaatc cttgatacca tcagtctcta ctcctccact
      961 tacatcgaca ccgatcgcat ttggtaacat attaatggca acagaaacgt tatcaggatt
     1021 caatccacca gcgataatga attttatctc gggatgactt gcagaccaac tggaaattgc
     1081 actccaattc aatttctcac cagtgccacc ttcaccagaa tcgaacaacg tcagcacatt
     1141 gtctacgtgt tcatacaggt ccagtagtaa ttcacaatcc tgtgggaact ggaacctctt
     1201 aatgattgga attgaagatg ggatcaaaga tctgtattct ttaatatctt catctccatg
     1261 taattgtatc acatctagat tatattcgtg gtacagttga aggacatcat caacggactg
     1321 atttctaaac accccgacta gtttagtacc tttcacgttc tcttgttggt gaactgcagt
     1381 tgaaatacct ttcgcaacag atgagtcaat ggttcttttc ctaccgggaa cacaaatgat
     1441 acctaagtaa tcagcaccat catccacagc agtctttgca gcttcaacgg tttgcaaacc
     1501 acacacttta acgagcatca cagtagattt cgcaagaggg tagctcgctc agagtaccca
     1561 agtaaatgat tagtaaactg atgtttgata gttcaatttt tcaatgaaat aaccttatat
     1621 taaaattgat attactatta tacaaaaata aagaataaag gatttgagtt tatacataaa
     1681 ataccattat tatttgttca gtgagagata ccggggtata tgggatgtgt gtagtgatac
     1741 catgcaatca tgtatcaaac atggaagaag tgaaggggga gaaagaaact tgaaaaaaag
     1801 aaaaccattt gaaaagagga aaagtatcta attaagatct ttcacctctt agtcttctgg
     1861 cctcgaggcg agctcgaatt catcgatgat atcagatcca ctagtggcct atgcggccgc
     1921 ggatctgccg gtctccctat agtgagtcgt attaatttcg ataagccagg ttaacctgca
     1981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2401 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2641 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3961 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4021 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4081 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4141 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     4201 actgagagtg caccatatgg acatattgtc gttagaacgc ggctacaatt aatacataac
     4261 cttatgtatc atacacatac gatttaggtg acactata
//
