LOCUS       40924_48173       17089 bp DNA     circular SYN 13-MAY-2021
DEFINITION  expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17089)
  AUTHORS   Ma X, Zhang Q, Zhu Q, Liu W, Chen Y, Qiu R, Wang B, Yang Z, Li H, 
            Lin Y, Xie Y, Shen R, Chen S, Wang Z, Chen Y, Guo J, Chen L, Zhao X,
            Dong Z, Liu YG
  TITLE     A robust CRISPR/Cas9 system for convenient, high-efficiency 
            multiplex genome editing in monocot and dicot plants.
  JOURNAL   Mol Plant. 2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007.
  PUBMED    25917172
REFERENCE   2  (bases 1 to 17089)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 17089)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2015 Apr 24. pii: S1674-2052(15)00204-X. doi: 
            10.1016/j.molp.2015.04.007."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..17089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(287..835)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(880..1556)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    1747..1768
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1783..1813
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1821..1837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1826..1848
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1845..1861
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1845..1861
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2009..3996
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             4003..4023
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4048..8148
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8149..8196
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8209..8461
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8579..8600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8615..8645
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8653..8669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        8711..8729
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             8803..9102
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    9315..9348
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(9392..9409)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(9392..9408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9401..9423)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9611..9635
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10936..11562
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11994..13064
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13133..13327
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13563..13585)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13671..13811
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13826..13843)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13997..14585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(14675..15466)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagtcaaatc tcggtgacgg
      301 gcaggaccgg acggggcggt accggcaggc tgaagtccag ctgccagaaa cccacgtcat
      361 gccagttccc gtgcttgaag ccggccgccc gcagcatgcc gcggggggca tatccgagcg
      421 cctcgtgcat gcgcacgctc gggtcgttgg gcagcccgat gacagcgacc acgctcttga
      481 agccctgtgc ctccagggac ttcagcaggt gggtgtagag cgtggagccc agtcccgtcc
      541 gctggtggcg gggggagacg tacacggtcg actcggccgt ccagtcgtag gcgttgcgtg
      601 ccttccaggg gcccgcgtag gcgatgccgg cgacctcgcc gtccacctcg gcgacgagcc
      661 agggatagcg ctcccgcaga cggacgaggt cgtccgtcca ctcctgcggt tcctgcggct
      721 cggtacggaa gttgaccgtg cttgtctcga tgtagtggtt gacgatggtg cagaccgccg
      781 gcatgtccgc ctcggtggca cggcggatgt cggccgggcg tcgttctggg ctcatggtag
      841 actcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
      901 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
      961 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     1021 ttctttttcc acgatgctct tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     1081 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     1141 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     1201 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     1261 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt
     1321 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     1381 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     1441 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     1501 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     1561 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     1621 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc
     1681 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1741 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     1801 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     1861 catgattacg aattcgagct cggtaccgta actataacgg tcctaaggta gcgaaggatc
     1921 cgctcgctac cttaagagag gatatccctc catcctataa tgtaggctat aggaactagg
     1981 gcaaggccgg ccatgcggcc gcaagctggg tgcagcgtga cccggtcgtg cccctctcta
     2041 gagataatga gcattgcatg tctaagttat aaaaaattac cacatatttt ttttgtcaca
     2101 cttgtttgaa gtgcagttta tctatcttta tacatatatt taaactttac tctacgaata
     2161 atataatcta tagtactaca ataatatcag tgttttagag aatcatataa atgaacagtt
     2221 agacatggtc taaaggacaa ttgagtattt tgacaacagg actctacagt tttatctttt
     2281 tagtgtgcat gtgttctcct ttttttttgc aaatagcttc acctatataa tacttcatcc
     2341 attttattag tacatccatt tagggtttag ggttaatggt ttttatagac taattttttt
     2401 agtacatcta ttttattcta ttttagcctc taaattaaga aaactaaaac tctattttag
     2461 tttttttatt taataattta gatataaaat agaataaaat aaagtgacta aaaattaaac
     2521 aaataccctt taagaaatta aaaaaactaa ggaaacattt ttcttgtttc gagtagataa
     2581 tgccagcctg ttaaacgccg tcgacgagtc taacggacac caaccagcga accagcagcg
     2641 tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg tcgctgcctc tggacccctc
     2701 tcgagagttc cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc
     2761 ggagcggcag acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca
     2821 gctacggggg attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa
     2881 atagacaccc cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac
     2941 acaaccagat ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt
     3001 cctccccccc cccctnctct accttctcta gatcggcgtt ccggtccatg gttagggccc
     3061 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc
     3121 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag
     3181 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca
     3241 tgattttttt tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat
     3301 gccgtgcact tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat
     3361 gtggtctggt tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg
     3421 gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag
     3481 atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg
     3541 catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg
     3601 ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt
     3661 ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat
     3721 atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc
     3781 atatgcagca tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt
     3841 atgttttata attattttga tcttgatata cttggatgat ggcatatgca gcagctatat
     3901 gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt
     3961 cgatgctcac cctgttgttt ggtgttactt ctgcagatgg ctcctaagaa gaagcggaag
     4021 gttggtattc acggggtgcc tgcggctgac aagaagtact ccatcggcct cgacatcggc
     4081 accaacagcg tcggctgggc ggtgatcacc gacgagtaca aggtcccgtc caagaagttc
     4141 aaggtcctgg gcaacaccga ccgccactcc atcaagaaga acctcatcgg cgccctcctc
     4201 ttcgactccg gcgagacggc ggaggcgacc cgcctcaagc gcaccgcccg ccgccgctac
     4261 acccgccgca agaaccgcat ctgctacctc caggagatct tctccaacga gatggcgaag
     4321 gtcgacgact ccttcttcca ccgcctcgag gagtccttcc tcgtggagga ggacaagaag
     4381 cacgagcgcc accccatctt cggcaacatc gtcgacgagg tcgcctacca cgagaagtac
     4441 cccactatct accaccttcg taagaagctt gttgactcta ctgataaggc tgatcttcgt
     4501 ctcatctacc ttgctctcgc tcacatgatc aagttccgtg gtcacttcct tatcgagggt
     4561 gaccttaacc ctgataactc cgacgtggac aagctcttca tccagctcgt ccagacctac
     4621 aaccagctct tcgaggagaa ccctatcaac gcttccggtg tcgacgctaa ggcgatcctt
     4681 tccgctaggc tctccaagtc caggcgtctc gagaacctca tcgcccagct ccctggtgag
     4741 aagaagaacg gtcttttcgg taacctcatc gctctctccc tcggtctgac ccctaacttc
     4801 aagtccaact tcgacctcgc tgaggacgct aagcttcagc tctccaagga tacctacgac
     4861 gatgatctcg acaacctcct cgctcagatt ggagatcagt acgctgatct cttccttgct
     4921 gctaagaacc tctccgatgc tatcctcctt tcggatatcc ttagggttaa cactgagatc
     4981 actaaggctc ctctttctgc ttccatgatc aagcgctacg acgagcacca ccaggacctc
     5041 accctcctca aggctcttgt tcgtcagcag ctccccgaga agtacaagga gatcttcttc
     5101 gaccagtcca agaacggcta cgccggttac attgacggtg gagctagcca ggaggagttc
     5161 tacaagttca tcaagccaat ccttgagaag atggatggta ctgaggagct tctcgttaag
     5221 cttaaccgtg aggacctcct taggaagcag aggactttcg ataacggctc tatccctcac
     5281 cagatccacc ttggtgagct tcacgccatc cttcgtaggc aggaggactt ctaccctttc
     5341 ctcaaggaca accgtgagaa gatcgagaag atccttactt tccgtattcc ttactacgtt
     5401 ggtcctcttg ctcgtggtaa ctcccgtttc gcttggatga ctaggaagtc cgaggagact
     5461 atcacccctt ggaacttcga ggaggttgtt gacaagggtg cttccgccca gtccttcatc
     5521 gagcgcatga ccaacttcga caagaacctc cccaacgaga aggtcctccc caagcactcc
     5581 ctcctctacg agtacttcac ggtctacaac gagctcacca aggtcaagta cgtcaccgag
     5641 ggtatgcgca agcctgcctt cctctccggc gagcagaaga aggctatcgt tgacctcctc
     5701 ttcaagacca accgcaaggt caccgtcaag cagctcaagg aggactactt caagaagatc
     5761 gagtgcttcg actccgtcga gatcagcggc gttgaggacc gtttcaacgc ttctctcggt
     5821 acctaccacg atctcctcaa gatcatcaag gacaaggact tcctcgacaa cgaggagaac
     5881 gaggacatcc tcgaggacat cgtcctcact cttactctct tcgaggatag ggagatgatc
     5941 gaggagaggc tcaagactta cgctcatctc ttcgatgaca aggttatgaa gcagctcaag
     6001 cgtcgccgtt acaccggttg gggtaggctc tcccgcaagc tcatcaacgg tatcagggat
     6061 aagcagagcg gcaagactat cctcgacttc ctcaagtctg atggtttcgc taacaggaac
     6121 ttcatgcagc tcatccacga tgactctctt accttcaagg aggatattca gaaggctcag
     6181 gtgtccggtc agggcgactc tctccacgag cacattgcta accttgctgg ttcccctgct
     6241 atcaagaagg gcatccttca gactgttaag gttgtcgatg agcttgtcaa ggttatgggt
     6301 cgtcacaagc ctgagaacat cgtcatcgag atggctcgtg agaaccagac tacccagaag
     6361 ggtcagaaga actcgaggga gcgcatgaag aggattgagg agggtatcaa ggagcttggt
     6421 tctcagatcc ttaaggagca ccctgtcgag aacacccagc tccagaacga gaagctctac
     6481 ctctactacc tccagaacgg tagggatatg tacgttgacc aggagctcga catcaacagg
     6541 ctttctgact acgacgtcga ccacattgtt cctcagtctt tccttaagga tgactccatc
     6601 gacaacaagg tcctcacgag gtccgacaag aacaggggta agtcggacaa cgtcccttcc
     6661 gaggaggttg tcaagaagat gaagaactac tggaggcagc ttctcaacgc taagctcatt
     6721 acccagagga agttcgacaa cctcacgaag gctgagaggg gtggcctttc cgagcttgac
     6781 aaggctggtt tcatcaagag gcagcttgtt gagacgaggc agattaccaa gcacgttgct
     6841 cagatcctcg attctaggat gaacaccaag tacgacgaga acgacaagct catccgcgag
     6901 gtcaaggtga tcaccctcaa gtccaagctc gtctccgact tccgcaagga cttccagttc
     6961 tacaaggtcc gcgagatcaa caactaccac cacgctcacg atgcttacct taacgctgtc
     7021 gttggtaccg ctcttatcaa gaagtaccct aagcttgagt ccgagttcgt ctacggtgac
     7081 tacaaggtct acgacgttcg taagatgatc gccaagtccg agcaggagat cggcaaggcc
     7141 accgccaagt acttcttcta ctccaacatc atgaacttct tcaagaccga gatcaccctc
     7201 gccaacggcg agatccgcaa gcgccctctt atcgagacga acggtgagac tggtgagatc
     7261 gtttgggaca agggtcgcga cttcgctact gttcgcaagg tcctttctat gcctcaggtt
     7321 aacatcgtca agaagaccga ggtccagacc ggtggcttct ccaaggagtc tatccttcca
     7381 aagagaaact cggacaagct catcgctagg aagaaggatt gggaccctaa gaagtacggt
     7441 ggtttcgact cccctactgt cgcctactcc gtcctcgtgg tcgccaaggt ggagaagggt
     7501 aagtcgaaga agctcaagtc cgtcaaggag ctcctcggca tcaccatcat ggagcgctcc
     7561 tccttcgaga agaacccgat cgacttcctc gaggccaagg gctacaagga ggtcaagaag
     7621 gacctcatca tcaagctccc caagtactct cttttcgagc tcgagaacgg tcgtaagagg
     7681 atgctggctt ccgctggtga gctccagaag ggtaacgagc ttgctcttcc ttccaagtac
     7741 gtgaacttcc tctacctcgc ctcccactac gagaagctca agggttcccc tgaggataac
     7801 gagcagaagc agctcttcgt ggagcagcac aagcactacc tcgacgagat catcgagcag
     7861 atctccgagt tctccaagcg cgtcatcctc gctgacgcta acctcgacaa ggtcctctcc
     7921 gcctacaaca agcaccgcga caagcccatc cgcgagcagg ccgagaacat catccacctc
     7981 ttcacgctca cgaacctcgg cgcccctgct gctttcaagt acttcgacac caccatcgac
     8041 aggaagcgtt acacgtccac caaggaggtt ctcgacgcta ctctcatcca ccagtccatc
     8101 accggtcttt acgagactcg tatcgacctt tcccagcttg gtggtgataa gcgtcctgct
     8161 gccaccaaaa aggccggaca ggctaagaaa aagaagtagg atcctcccga tcgttcaaac
     8221 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     8281 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     8341 atgaggtggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     8401 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
     8461 cgggagcacc ggtaaggcgc gccgtagtgc tcgagagacc tctgaagtgg ccgattcatt
     8521 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8581 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8641 tgttgtgtgg aattgtgagc ggataacaat ttcacacaag aaacagctat gaccatgatt
     8701 acgccaagct atttaggtga cactatagaa tactcaagct atgcatcaag ctcaatgggt
     8761 ctagtctgta gatacccatc acactggcga ccgctcgaac atcagtttaa ggtttacacc
     8821 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     8881 ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     8941 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     9001 atggccagtg tgcctgtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     9061 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggcctgaat
     9121 ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgagcgt gggtctcgcg
     9181 gtatcattgg cgcgcctctc gagctagcgg ccgcatgcat cgatctccta catcgtataa
     9241 attagcctat acgaagttat tgcatctatg tcgggtgcgg agaaagaggt aatgaaatgg
     9301 cagtattaga tctgataact tcgtataatg tatgctatac gaagttatga ctgcaggtcg
     9361 acacccataa tagctgtttg ccaagcttgg cactggccgt cgttttacaa cgtcgtgact
     9421 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     9481 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     9541 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
     9601 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     9661 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
     9721 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
     9781 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
     9841 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
     9901 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
     9961 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
    10021 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
    10081 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
    10141 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
    10201 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
    10261 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
    10321 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
    10381 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
    10441 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
    10501 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
    10561 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
    10621 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
    10681 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc
    10741 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
    10801 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
    10861 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
    10921 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
    10981 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
    11041 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
    11101 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
    11161 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
    11221 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
    11281 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
    11341 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
    11401 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
    11461 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
    11521 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
    11581 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
    11641 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
    11701 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
    11761 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
    11821 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
    11881 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
    11941 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
    12001 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
    12061 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
    12121 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
    12181 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
    12241 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
    12301 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
    12361 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
    12421 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
    12481 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
    12541 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
    12601 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
    12661 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
    12721 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
    12781 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
    12841 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
    12901 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
    12961 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
    13021 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
    13081 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa agttctcttt cctgtggata
    13141 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
    13201 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
    13261 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
    13321 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
    13381 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
    13441 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
    13501 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
    13561 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
    13621 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
    13681 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
    13741 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    13801 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    13861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    13921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    13981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    14041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    14101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    14161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    14221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    14281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    14341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    14401 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    14461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    14521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    14581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    14641 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
    14701 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    14761 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    14821 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    14881 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    14941 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    15001 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    15061 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    15121 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    15181 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    15241 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    15301 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    15361 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    15421 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    15481 ctcttttcta cagtatttaa agatacccca agaagctaag gaaggtgcga acaagtccct
    15541 gatatgagat catgtttgtc atctggagcc atagaacagg gttcatcatg agtcatcaac
    15601 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
    15661 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
    15721 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
    15781 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
    15841 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgccggac cgcaccacca
    15901 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
    15961 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
    16021 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
    16081 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
    16141 ccaagagtgg cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc
    16201 agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
    16261 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
    16321 ccggcaaggt aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg
    16381 aatacatgaa agccagcatc cgggccaggg tggagcaccc atttcgcatc atcaagcgac
    16441 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
    16501 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
    16561 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
    16621 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
    16681 ccgagaagaa tttccccgct tattcgcacc ttccctaatt ataacaagac gaactccaat
    16741 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    16801 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
    16861 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
    16921 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
    16981 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
    17041 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctgg
//
