LOCUS       62056_23140        8643 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8643)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(996..1853)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1854..1958)
                     /label=AmpR promoter
     rep_origin      complement(2704..3217)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     enhancer        3815..4118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4119..4322
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    complement(8269..8403)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   8508..8643
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2281 cgcatcagga attccaacat ccaataaatc atacaggcaa ggcaaagaat tagcaaaatt
     2341 aagcaataaa gcctcagagc ataaagctaa atcggttgta ccaaaaacat tatgaccctg
     2401 taatactttt gcgggagaag cctttatttc aacgcaagga taaaaatttt tagaaccctc
     2461 atatatttta aatgcaatgc ctgagtaatg tgtaggtaaa gattcaaacg ggtgagaaag
     2521 gccggagaca gtcaaatcac catcaatatg atattcaacc gttctagctg ataaattcat
     2581 gccggagagg gtagctattt ttgagaggtc tctacaaagg ctatcaggtc attgcctgag
     2641 agtctggagc aaacaagaga atcgatgaac ggtaatcgta aaactagcat gtcaatcata
     2701 tgtaccccgg ttgataatca gaaaagcccc aaaaacagga agattgtata agcaaatatt
     2761 taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     2821 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     2881 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     2941 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3001 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3061 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3121 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3181 acacccgccg cgcttaatgc gccgctacag ggcgcgtact atggttgctt tgacgagcac
     3241 gtataacgtg ctttcctcgt tagaatcaga gcgggagcta aacaggaggc cgattaaagg
     3301 gattttagac aggaacggta cgccagaatc ctgagaagtg tttttataat cagtgaggcc
     3361 accgagtaaa agagtctgtc catcacgcaa attaaccgtt gtcgcaatac ttctttgatt
     3421 agtaataaca tcacttgcct gagtagaaga actcaaacta tcggccttgc tggtaatatc
     3481 cagaacaata ttaccgccag ccattgcaac aggaaaaacg ctcatggaaa tacctacatt
     3541 ttgacgctca atcgtctgga attccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3601 cgatcggtgc gggcctcttc gctattacgc cagctggcgc gctcgctcgc tcactgaggc
     3661 cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg
     3721 agcgcgcaga gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa
     3781 cccgccatgc tacttatcta cggccgcggt accgcgttac ataacttacg gtaaatggcc
     3841 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3901 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3961 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     4021 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     4081 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     4141 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4201 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4261 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     4321 ctcgtttagt gaaccgtcta gacggccgcg gtttttttta tcgctgcctt gatatacact
     4381 ttccaccatg ctttggtggg aagaagtaga ggactgttat gaaagagaag atgttcaaaa
     4441 gaaaacattc acaaaatggg taaatgcaca attttctaag tttgggaagc agcatattga
     4501 gaacctcttc agtgacctac aggatgggag gcgcctccta gacctcctcg aaggcctgac
     4561 agggcaaaaa ctgccaaaag aaaaaggatc cacaagagtt catgccctga acaatgtcaa
     4621 caaggcactg cgggttttgc agaacaataa tgttgattta gtgaatattg gaagtactga
     4681 catcgtagat ggaaatcata aactgactct tggtttgatt tggaatataa tcctccactg
     4741 gcaggtcaaa aatgtaatga aaaatatcat ggctggattg caacaaacca acagtgaaaa
     4801 gattctcctg agctgggtcc gacaatcaac tcgtaattat ccacaggtta atgtaatcaa
     4861 cttcaccacc agctggtctg atggcctggc tttgaatgct ctcatccata gtcataggcc
     4921 agacctattt gactggaata gtgtggtttg ccagcagtca gccacacaac gactggaaca
     4981 tgcattcaac atcgccagat atcaattagg catagagaaa ctactcgatc ctgaagatgt
     5041 tgataccacc tatccagata agaagtccat cttaatgtac atcacatcac tcttccaagt
     5101 tttgcctcaa caagtgagca ttgaagccat ccaggaagtg gaaatgttgc caaggccacc
     5161 taaagtgact aaagaagaac attttcagtt acatcatcaa atgcactatt ctcaacagat
     5221 cacggtcagt ctagcacagg gatatgagag aacttcttcc cctaagcctc gattcaagag
     5281 ctatgcctac acacaggctg cttatgtcac cacctctgac cctacacgga gcccatttcc
     5341 ttcacagcat ttggaagctc ctgaagacaa gtcatttggc agttcattga tggagagtga
     5401 agtaaacctg gaccgttatc aaacagcttt agaagaagta ttatcgtggc ttctttctgc
     5461 tgaggacaca ttgcaagcac aaggagagat ttctaatgat gtggaagtgg tgaaagacca
     5521 gtttcatact catgaggggt acatgatgga tttgacagcc catcagggcc gggttggtaa
     5581 tattctacaa ttgggaagta agctgattgg aacaggaaaa ttatcagaag atgaagaaac
     5641 tgaagtacaa gagcagatga atctcctaaa ttcaagatgg gaatgcctca gggtagctag
     5701 catggaaaaa caaagcaatt tacatagagt tttaatggat ctccagaatc agaaactgaa
     5761 agagttgaat gactggctaa caaaaacaga agaaagaaca aggaaaatgg aggaagagcc
     5821 tcttggacct gatcttgaag acctaaaacg ccaagtacaa caacataagg tgcttcaaga
     5881 agatctagaa caagaacaag tcagggtcaa ttctctcact cacatggtgg tggtagttga
     5941 tgaatctagt ggagatcacg caactgctgc tttggaagaa caacttaagg tattgggaga
     6001 tcgatgggca aacatctgta gatggacaga agaccgctgg gttcttttac aagacgaaat
     6061 ttcttatgtg ccttctactt atttgactga aatcactcat gtctcacaag ccctattaga
     6121 agtggaacaa cttctcaatg ctcctgacct ctgtgctaag gactttgaag atctctttaa
     6181 gcaagaggag tctctgaaga atataaaaga tagtctacaa caaagctcag gtcggattga
     6241 cattattcat agcaagaaga cagcagcatt gcaaagtgca acgcctgtgg aaagggtgaa
     6301 gctacaggaa gctctctccc agcttgattt ccaatgggaa aaagttaaca aaatgtacaa
     6361 ggaccgacaa gggcgatttg acagatctgt tgagaaatgg cggcgttttc attatgatat
     6421 aaagatattt aatcagtggc taacagaagc tgaacagttt ctcagaaaga cacaaattcc
     6481 tgagaattgg gaacatgcta aatacaaatg gtatcttaag gaactccagg atggcattgg
     6541 gcagcggcaa actgttgtca gaacattgaa tgcaactggg gaagaaataa ttcagcaatc
     6601 ctcaaaaaca gatgccagta ttctacagga aaaattggga agcctgaatc tgcggtggca
     6661 ggaggtctgc aaacagctgt cagacagaaa aaagaggcta gaagaaaccc ttgaaagact
     6721 ccaggaactt caagaggcca cggatgagct ggacctcaag ctgcgccaag ctgaggtgat
     6781 caagggatcc tggcagcccg tgggcgatct cctcattgac tctctccaag atcacctcga
     6841 gaaagtcaag gcacttcgag gagaaattgc gcctctgaaa gagaacgtga gccacgtcaa
     6901 tgaccttgct cgccagctta ccactttggg cattcagctc tcaccgtata acctcagcac
     6961 tctggaagac ctgaacacca gatggaagct tctgcaggtg gccgtcgagg accgagtcag
     7021 gcagctgcat gaagcccaca gggactttgg tccagcatct cagcactttc tttccacgtc
     7081 tgtccagggt ccctgggaga gagccatctc gccaaacaaa gtgccctact atatcaacca
     7141 cgagactcaa acaacttgct gggaccatcc caaaatgaca gagctctacc agtctttagc
     7201 tgacctgaat aatgtcagat tctcagctta taggactgcc atgaaactcc gaagactgca
     7261 gaaggccctt tgcttggatc tcttgagcct gtcagctgca tgtgatgcct tggaccagca
     7321 caacctcaag caaaatgacc agcccatgga tatcctgcag attattaatt gtttgaccac
     7381 tatttatgac cgcctggagc aagagcacaa caatttggtc aacgtccctc tctgcgtgga
     7441 tatgtgtctg aactggctgc tgaatgttta tgatacggga cgaacaggga ggatccgtgt
     7501 cctgtctttt aaaactggca tcatttccct gtgtaaagca catttggaag acaagtacag
     7561 ataccttttc aagcaagtgg caagttcaac aggattttgt gaccagcgca ggctgggcct
     7621 ccttctgcat gattctatcc aaattccaag acagttgggt gaagttgcat cctttggggg
     7681 cagtaacatt gagccaagtg tccggagctg cttccaattt gctaataata agccagagat
     7741 cgaagcggcc ctcttcctag actggatgag actggaaccc cagtccatgg tgtggctgcc
     7801 cgtcctgcac agagtggctg ctgcagaaac tgccaagcat caggccaaat gtaacatctg
     7861 caaagagtgt ccaatcattg gattcaggta caggagtcta aagcacttta attatgacat
     7921 ctgccaaagc tgcttttttt ctggtcgagt tgcaaaaggc cataaaatgc actatcccat
     7981 ggtggaatat tgcactccga ctacatcagg agaagatgtt cgagactttg ccaaggtact
     8041 aaaaaacaaa tttcgaacca aaaggtattt tgcgaagcat ccccgaatgg gctacctgcc
     8101 agtgcagact gtcttagagg gggacaacat ggaaactgac acaatgtagg aagtcttttc
     8161 cacatggcag atgatttggg cagagcgatg gagtccttag tatcagtcat gacagatgaa
     8221 gaaggagcag aataaatgtt ttacaactcc tgattcccgc atgcggccga tccagacatg
     8281 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     8341 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     8401 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     8461 ttttgcggcc gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
     8521 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     8581 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
     8641 ctg
//
