LOCUS       40924_48163       12607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  C-terminal TAP T-DNA vector pYL436, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12607)
  AUTHORS   Rubio V, Shen Y, Saijo Y, Liu Y, Gusmaroli G, Dinesh-Kumar SP, Deng 
            XW.
  TITLE     An alternative tandem affinity purification strategy applied to 
            Arabidopsis protein complex isolation
  JOURNAL   Plant J. 41 (5), 767-778 (2005)
  PUBMED    15703063
REFERENCE   2  (bases 1 to 12607)
  AUTHORS   Liu Y, Dinesh-Kumar SP.
  TITLE     A C-terminal tandem affinity purification (TAP) T-DNA vector pYL436
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 12607)
  AUTHORS   Liu Y, Dinesh-Kumar SP.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-AUG-2004) MCDB, Yale University, New Haven, CT 06520, 
            USA
REFERENCE   4  (bases 1 to 12607)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 12607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2005"; volume: "41"; issue: "5"; pages: "767-778"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (28-AUG-2004) MCDB, Yale University, New Haven, CT 06520, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..769
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    791..846
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     protein_bind    845..969
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1143..1245
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1246..1902
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2247..2549
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2696..2716)
                     /label=attB2
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             2755..2784
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2791..2820
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2827..2856
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2875..2904
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2911..2940
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2947..2976
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2995..3024
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3031..3060
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3067..3096
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3163..3180
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             3181..3204
                     /codon_start=1
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
                     /translation="LEVLFQGP"
     CDS             3244..3417
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             3421..3591
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPK"
     terminator      3675..3927
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3943..3959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3967..3983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3991..4021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4036..4057)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4222..4246
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5546..6172
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6609..7673
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7742..7936
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8280..8420
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8606..9194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9441..10229)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    10757..10781
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10879..11053)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11091..11621)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(11681..12359)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     primer_bind     12588..12604
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagaggc cggtctagag
      781 tatttttaca acaattacca acaacaacaa acaacaaaca acattacaat tactatttac
      841 aattacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa atatcaatat
      901 attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac aacatatcca
      961 gtcactatgg cggccgcggg tgatgctgcc aacttagcgg ccgctaagtt ggcagcatca
     1021 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
     1081 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     1141 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     1201 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     1261 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     1321 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     1381 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     1441 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     1501 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     1561 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     1621 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     1681 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     1741 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     1801 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     1861 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaatctag aggatccggc
     1921 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
     1981 tatactgata tgtatacccg aagtatgtca aaaagaggtg tgctatgaag cagcgtatta
     2041 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
     2101 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
     2161 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
     2221 tgctgacgag aacagggact ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
     2281 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
     2341 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
     2401 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
     2461 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
     2521 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctccgttata cacagccagt
     2581 ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
     2641 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
     2701 ttcttgtaca aagtggttga gctcaagctt gcgggccgct ctagaactag tggtgaacaa
     2761 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
     2821 aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatcctctag aggtgaacaa
     2881 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
     2941 aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatcctctag aggtgaacaa
     3001 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
     3061 aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatccactag tggatccccc
     3121 gggctgcagc ctagggatta cgatatccca acgaccgcca gtcatcacca tcaccatcac
     3181 ctggaagttc tgttccaggg gcccgagctt aagacggccg ccctagcgca acacgatgaa
     3241 gccgtagaca acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta
     3301 cctaacttaa acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc
     3361 caaagcgcta accttttagc agaagctaaa aagctaaatg atgctcaggc gccgaaagta
     3421 gacaacaaat tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac
     3481 ttaaacgaag aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc
     3541 gctaaccttt tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacgcg
     3601 aattcgagct cggtatgacc caaacccgct gatcctagag gacgtctcga ggtaccgccc
     3661 gggcgagctc ggaagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     3721 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     3781 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     3841 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     3901 gcgcggtgtc atctatgtta ctagatcgaa ttcgtaatca tggtcatagc tgtttcctgt
     3961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4141 aggcggtttg cgtattggct agagcagctt gagcttggat cagattgtcg tttcccgcct
     4201 tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     4261 cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     4321 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     4381 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     4441 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     4501 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     4561 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     4621 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     4681 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     4741 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     4801 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     4861 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     4921 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc
     4981 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     5041 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     5101 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     5161 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     5221 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     5281 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     5341 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     5401 cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     5461 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     5521 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     5581 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     5641 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     5701 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     5761 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     5821 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     5881 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     5941 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     6001 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     6061 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     6121 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     6181 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     6241 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     6301 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     6361 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     6421 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     6481 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     6541 aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     6601 ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     6661 tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     6721 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     6781 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     6841 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     6901 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     6961 acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg gattacgacc
     7021 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     7081 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     7141 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     7201 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     7261 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     7321 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     7381 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     7441 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     7501 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     7561 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     7621 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     7681 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     7741 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     7801 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     7861 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     7921 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     7981 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     8041 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     8101 cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat
     8161 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     8221 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     8281 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     8341 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     8401 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     8461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     8521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     8581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     9001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     9061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     9121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     9181 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     9241 aaaactcacg ttaagggatt ttggtcatgc atgatatatc tcccaatttg tgtagggctt
     9301 attatgcacg cttaaaaata ataaaagcag acttgacctg atagtttggc tgtgagcaat
     9361 tatgtgctta gtgcatctaa tcgcttgagt taacgccggc gaagcggcgt cggcttgaac
     9421 gaatttctag ctagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg
     9481 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     9541 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     9601 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     9661 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     9721 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     9781 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     9841 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     9901 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     9961 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
    10021 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
    10081 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
    10141 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
    10201 gatacttcgg cgatcaccgc ttcccccatg atgtttaact ttgttttagg gcgactgccc
    10261 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
    10321 tgctgcttgg atgcccgagg catagactgt accccaaaaa aacatgtcat aacaagaagc
    10381 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
    10441 gcgtgacggc agttacgcta cttgcattac agcttacgaa ccgaacgagg cttatgtcca
    10501 ctgggttcgt gcccgaattg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    10561 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    10621 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    10681 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    10741 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    10801 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga
    10861 tctataggga ctttaggtga tctggatttt agtactggat tttggtttta ggaattagaa
    10921 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
    10981 aacacgtgag cgaaacccta taagaaccct aattccctta tctgggaact actcacacat
    11041 tattatggag aaactcgatg tcgatcgact ctagctagaa cgaattgtta ggtggcggta
    11101 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
    11161 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
    11221 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
    11281 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg
    11341 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
    11401 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
    11461 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
    11521 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
    11581 cgactgccct gctgcgtaac atcgttgctg ctgcgtacca tggtcgatcg acagatctgc
    11641 gaatcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
    11701 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
    11761 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
    11821 cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag
    11881 aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt
    11941 ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt
    12001 ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat
    12061 ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttatca catcaatcca
    12121 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    12181 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    12241 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    12301 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    12361 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    12421 gctccaccat gttggcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    12481 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    12541 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    12601 cagtgcc
//
