LOCUS       V001880                 6275 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001880
VERSION     V001880
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Liu Y, Charpin-El Hamri G, Ye H, Fussenegger M.
  TITLE     A synthetic free fatty acid-regulated transgene switch in mammalian
            cells and mice
  JOURNAL   Nucleic Acids Res. (2018) In press
   PUBMED   30219861
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Liu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2018) Department of Biosystems Science and
            Engineering, ETH Zurich, Mattenstrasse 26, basel 4058, Switzerland
REFERENCE   3  (bases 1 to 6275)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUL-2018) Department of Biosystems Science and Engineering, ETH
            Zurich, Mattenstrasse 26, basel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..919
                     /gene="FFAR1"
                     /label="Free fatty acid receptor 1"
                     /note="Free fatty acid receptor 1 from Homo sapiens.
                     Accession#: O14842"
     polyA_signal    960..1184
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1230..1658
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1672..2001
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2068..2859
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3036..3169
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3206..3222)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3230..3246)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3254..3284)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3299..3320)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3608..4193)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4367..5224)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5225..5329)
                     /label="AmpR promoter"
     enhancer        5595..5974
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5975..6178
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        6223..6241
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tcgaatgaat tcgcccacca tggacctgcc cccgcagctc tccttcggcc tctatgtggc
       61 cgcctttgcg ctgggcttcc cgctcaacgt cctggccatc cgaggcgcga cggcccacgc
      121 ccggctccgt ctcaccccta gcctggtcta cgccctgaac ctgggctgct ccgacctgct
      181 gctgacagtc tctctgcccc tgaaggcggt ggaggcgcta gcctccgggg cctggcctct
      241 gccggcctcg ctgtgccccg tcttcgcggt ggcccacttc ttcccactct atgccggcgg
      301 gggcttcctg gccgccctga gtgcaggccg ctacctggga gcagccttcc ccttgggcta
      361 ccaagccttc cggaggccgt gctattcctg gggggtgtgc gcggccatct gggccctcgt
      421 cctgtgtcac ctgggtctgg tctttgggtt ggaggctcca ggaggctggc tggaccacag
      481 caacacctcc ctgggcatca acacaccggt caacggctct ccggtctgcc tggaggcctg
      541 ggacccggcc tctgccggcc cggcccgctt cagcctctct ctcctgctct tttttctgcc
      601 cttggccatc acagccttct gctacgtggg ctgcctccgg gcactggccc gctccggcct
      661 gacgcacagg cggaagctgc gggccgcctg ggtggccggc ggggccctcc tcacgctgct
      721 gctctgcgta ggaccctaca acgcctccaa cgtggccagc ttcctgtacc ccaatctagg
      781 aggctcctgg cggaagctgg ggctcatcac gggtgcctgg agtgtggtgc ttaatccgct
      841 ggtgaccggt tacttgggaa ggggtcctgg cctgaagaca gtgtgtgcgg caagaacgca
      901 agggggcaag tcccagaagt aatctagagg gcccgtttaa acccgctgat cagcctcgac
      961 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1021 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1081 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1141 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     1201 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     1261 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1321 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     1441 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     1501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     1561 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     1621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     1681 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     1741 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     1801 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     1861 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     1921 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     1981 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     2041 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     2101 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     2161 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     2221 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     2281 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     2341 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     2401 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     2461 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     2521 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     2581 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     2641 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     2701 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     2761 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     2821 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     2881 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     2941 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     3001 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     3061 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     3121 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     3181 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3301 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3421 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4141 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4201 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4261 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4441 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4501 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4561 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4621 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4681 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4741 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4801 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4861 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4921 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4981 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5041 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5101 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5161 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5221 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5281 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5341 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     5401 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     5461 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     5521 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     5581 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     5641 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     5701 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     5761 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     5821 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     5881 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     5941 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     6001 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     6061 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     6121 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     6181 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
     6241 ggagacccaa gctggctagc gtttaaactt aagct
//
