LOCUS       40924_48148        5821 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pYL2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Pomraning KR.
  TITLE     Yarrowia lipolytica functional genomics toolbox
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Pomraning KR.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2016) Environmental Molecular Science Lab, Pacific
            Northwest National Lab, 902 Battelle Blvd, Richland, WA 99352, USA
REFERENCE   3  (bases 1 to 5821)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5821)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-JAN-2016) Environmental Molecular Science Lab, Pacific Northwest
            National Lab, 902 Battelle Blvd, Richland, WA 99352, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5821
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     regulatory      382..1382
                     /gene="exp1"
                     /regulatory_class="promoter"
     gene            382..1382
                     /gene="exp1"
                     /label=exp1
     CDS             1431..2141
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     regulatory      2175..2579
                     /gene="tef"
                     /regulatory_class="promoter"
     gene            2175..2579
                     /gene="tef"
                     /label=tef
     CDS             2592..3614
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    3662..3906
                     /note="modified version of ARS68, contains centromere and
                     origin of replication sequences for Yarrowia lipolytica"
     rep_origin      complement(4137..4725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4902..5693)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5694..5798)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcatc agctggaatt caaggagttt ggcgcccgtt ttttcgagcc ccacacgttt
      421 cggtgagtat gagcggcggc agattcgagc gtttccggtt tccgcggctg gacgagagcc
      481 catgatgggg gctcccacca ccagcaatca gggccctgat tacacaccca cctgtaatgt
      541 catgctgttc atcgtggtta atgctgctgt gtgctgtgtg tgtgtgttgt ttggcgctca
      601 ttgttgcgtt atgcagcgta caccacaata ttggaagctt attagccttt ctattttttc
      661 gtttgcaagg cttaacaaca ttgctgtgga gagggatggg gatatggagg ccgctggagg
      721 gagtcggaga ggcgttttgg agcggcttgg cctggcgccc agctcgcgaa acgcacctag
      781 gaccctttgg cacgccgaaa tgtgccactt ttcagtctag taacgcctta cctacgtcat
      841 tccatgcatg catgtttgcg ccttttttcc cttgcccttg atcgccacac agtacagtgc
      901 actgtacagt ggaggttttg ggggggtctt agatgggagc taaaagcggc ctagcggtac
      961 actagtggga ttgtatggag tggcatggag cctaggtgga gcctgacagg acgcacgacc
     1021 ggctagcccg tgacagacga tgggtggctc ctgttgtcca ccgcgtacaa atgtttgggc
     1081 caaagtcttg tcagccttgc ttgcgaacct aattcccaat tttgtcactt cgcaccccca
     1141 ttgatcgagc cctaacccct gcccatcagg caatccaatt aagctcgcat tgtctgcctt
     1201 gtttagtttg gctcctgccc gtttcggcgt ccacttgcac aaacacaaac aagcattata
     1261 tataaggctc gtctctccct cccaaccaca ctcacttttt tgcccgtctt cccttgctaa
     1321 cacaaaagtc aagaacacaa acaaccaccc caaccccctt acacacaaga catatctaca
     1381 gcggtacccc cggggatatc ggcggaggcg gcggaggcgg aggcggaggc agaaagggtg
     1441 aggagctgtt tacaggcgtt gtgcccatcc ttgtggagct tgacggcgat gtcaacggtc
     1501 ataagttctc cgttcgagga gaaggcgagg gtgatgccac caacggaaag ctcaccctta
     1561 agttcatctg cactaccggt aagctgcccg tgccttggcc taccctggtc accactctca
     1621 cctatggtgt gcagtgcttc gcccgatacc ccgatcacat gaagcagcat gactttttta
     1681 agtctgccat gcctgaaggc tacgtccagg agcgaaccat ctctttcaag gatgatggaa
     1741 cctacaagac tcgagctgag gtcaagtttg agggtgatac tctggtcaac cgaatcgagc
     1801 tgaagggaat tgattttaag gaggatggaa acatcctcgg tcacaagctc gagtacaact
     1861 tcaactccca caacgtctac atcaccgctg ataagcagaa gaacggaatt aaggctaact
     1921 tcaagattcg acacaatgtc gaagacggtt ccgttcagct cgctgaccac taccagcaga
     1981 acactcctat tggtgacgga cccgtcctgc tgcccgacaa ccattacctc tcgacccagt
     2041 cggtcctttc taaggaccct aacgagaagc gagaccacat ggtccttctg gagttcgtca
     2101 ctgccgctgg aattacccac ggcatggatg agctttacaa gtaagatatc aataaaggat
     2161 cctctagact gcagagagac cgggttggcg gcgcatttgt gtcccaaaaa acagccccaa
     2221 ttgccccaat tgacccaaat tgacccagta gcgggcccaa ccccggcgag agcccccttc
     2281 tccccacata tcaaacctcc cccggttccc acacttgccg ttaagggcgt agggtactgc
     2341 agtctggaat ctacgcttgt tcagactttg tactagtttc tttgtctggc catccgggta
     2401 acccatgccg gacgcaaaat agactactga aaattttttt gctttgtggt tgggacttta
     2461 gccaagggta taaaagacca ccgtccccga attacctttc ctcttctttt ctctctctcc
     2521 ttgtcaactc acacccgaaa tcgttaagca tttccttctg agtataagaa tcattcaaaa
     2581 gatctgagct catgaagaag cccgagctga ccgctacatc tgtggagaag tttctgatcg
     2641 agaagttcga ctctgtctcc gacctcatgc agctctccga aggtgaggag tcccgagctt
     2701 tctcgttcga cgtgggtgga agaggatacg tccttcgagt taactcgtgt gcagatggat
     2761 tctacaagga tcggtacgtg taccgacatt ttgctagcgc cgccctgccc attcctgagg
     2821 tgcttgacat cggtgagttt tcggagtccc ttacctactg tatctcccgg cgagcccagg
     2881 gagttacact ccaggacctg cccgagaccg agctgcccgc cgtcctccag cccgtggctg
     2941 aggctatgga cgccatcgcc gctgctgatc tgagccaaac ctccggattt ggtccttttg
     3001 gccctcaggg cattggtcag tacacaactt ggcgggattt catctgcgct atcgccgatc
     3061 ctcacgttta ccactggcaa accgttatgg acgacaccgt gagcgcctct gtcgcccagg
     3121 ctctcgacga gctgatgctt tgggctgagg actgtcccga ggtcagacac ctggtccacg
     3181 ctgactttgg atctaacaac gttctcactg ataacggcag aattaccgct gtcatcgact
     3241 ggtctgaggc aatgtttgga gattcccagt acgaggtggc aaacatcttc ttttggcgac
     3301 cctggcttgc ctgtatggaa cagcagaccc gatatttcga acgacgacat cccgagcttg
     3361 ctggttctcc tcgactgcgg gcttatatgc tccgaattgg acttgatcaa ctctaccagt
     3421 ctctggttga cggtaacttt gacgacgccg cctgggctca gggacgatgt gacgccattg
     3481 tccgaagcgg agccggtact gtgggccgaa cccagatcgc ccgacgatct gccgctgtct
     3541 ggaccgatgg ttgtgttgag gtcctggcag actctggaaa cagacgacct tctacccgac
     3601 ccagagccaa agagtaagga tcctctagac tgcagggatt accgcaacac gctataataa
     3661 accaattatg ttatgtctga taaaaggatg taacataggc aagctgctcg tgagtgttga
     3721 gtacgaacct tagatccaaa tcacccgcac cctacggata tacttgcttg aatatacagt
     3781 agtatttgta ataagttcat aattaatatc ttaccaaatt aggtaatctg caaaagttca
     3841 gactgtgaaa tgtaacattt tatatatcaa gctctattta atgcctcaca gtagttaaca
     3901 taaagacagc tgctgggcct catgggcctt cctttcactg cccgctttcc agtcgggaaa
     3961 cctgtcgtgc cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc
     4021 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa
     4081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4861 atctaaagta tatatgagta aacttggtct gacagttatt agaaaaattc atccagcaga
     4921 cgataaaacg caatacgctg gctatccggt gccgcaatgc catacagcac cagaaaacga
     4981 tccgcccatt cgccgcccag ttcttccgca atatcacggg tggccagcgc aatatcctga
     5041 taacgatccg ccacgcccag acggccgcaa tcaataaagc cgctaaaacg gccattttcc
     5101 accataatgt tcggcaggca cgcatcacca tgggtcacca ccagatcttc gccatccggc
     5161 atgctcgctt tcagacgcgc aaacagctct gccggtgcca ggccctgatg ttcttcatcc
     5221 agatcatcct gatccaccag gcccgcttcc atacgggtac gcgcacgttc aatacgatgt
     5281 ttcgcctgat gatcaaacgg acaggtcgcc gggtccaggg tatgcagacg acgcatggca
     5341 tccgccataa tgctcacttt ttctgccggc gccagatggc tagacagcag atcctgaccc
     5401 ggcacttcgc ccagcagcag ccaatcacgg cccgcttcgg tcaccacatc cagcaccgcc
     5461 gcacacggaa caccggtggt ggccagccag ctcagacgcg ccgcttcatc ctgcagctcg
     5521 ttcagcgcac cgctcagatc ggttttcaca aacagcaccg gacgaccctg cgcgctcaga
     5581 cgaaacaccg ccgcatcaga gcagccaatg gtctgctgcg cccaatcata gccaaacaga
     5641 cgttccaccc acgctgccgg gctacccgca tgcaggccat cctgttcaat catactcttc
     5701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5821 c
//
