LOCUS       V001882                 9748 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001882
VERSION     V001882
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Pomraning KR.
  TITLE     localization of lipid biogenesis in yarrowia lipolytica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   Pomraning KR.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2016) Environmental Molecular Science Lab, Pacific
            Northwest National Lab, 902 Battelle Blvd, Richland, WA 99352, USA
REFERENCE   3  (bases 1 to 9748)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-JAN-2016) Environmental Molecular Science Lab, Pacific Northwest
            National Lab, 902 Battelle Blvd, Richland, WA 99352, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             40..750
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     CDS             complement(1031..2245)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Yarrowia
                     lipolytica (strain CLIB 122 / E 150). Accession#: P18120"
     misc_feature    3037..3281
                     /note="modified version of ARS68, contains centromere and
                     origin of replication sequences for Yarrowia lipolytica"
     rep_origin      complement(3512..4100)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4277..5068)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5069..5173)
                     /label="AmpR promoter"
     primer_bind     5489..5505
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5516..5534
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5539..5550
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             complement(8036..8047)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
     regulatory      8995..9739
                     /gene="exp1"
                     /regulatory_class="promoter"
     gene            8995..9739
                     /gene="exp1"
                     /label="exp1"
ORIGIN
        1 ggggatatcg gcggaggcgg cggaggcgga ggcggaggca gaaagggtga ggagctgttt
       61 acaggcgttg tgcccatcct tgtggagctt gacggcgatg tcaacggtca taagttctcc
      121 gttcgaggag aaggcgaggg tgatgccacc aacggaaagc tcacccttaa gttcatctgc
      181 actaccggta agctgcccgt gccttggcct accctggtca ccactctcac ctatggtgtg
      241 cagtgcttcg cccgataccc cgatcacatg aagcagcatg acttttttaa gtctgccatg
      301 cctgaaggct acgtccagga gcgaaccatc tctttcaagg atgatggaac ctacaagact
      361 cgagctgagg tcaagtttga gggtgatact ctggtcaacc gaatcgagct gaagggaatt
      421 gattttaagg aggatggaaa catcctcggt cacaagctcg agtacaactt caactcccac
      481 aacgtctaca tcaccgctga taagcagaag aacggaatta aggctaactt caagattcga
      541 cacaatgtcg aagacggttc cgttcagctc gctgaccact accagcagaa cactcctatt
      601 ggtgacggac ccgtcctgct gcccgacaac cattacctct cgacccagtc ggtcctttct
      661 aaggacccta acgagaagcg agaccacatg gtccttctgg agttcgtcac tgccgctgga
      721 attacccacg gcatggatga gctttacaag taagatatca ataaaggatc cgtgcagtcg
      781 ccagcttaaa gatatctatc cacatcagcc acaactccct tcctttaata aaccgactac
      841 acccttggct attgaggtta tgagtgaata tactgtagac aagacacttt caagaagact
      901 gtttccaaaa cgtaccactg tcctccacta caaacacacc caatctgctt cttctagtca
      961 aggttgctac accggtaaat tataaatcat catttcatta gcagggcagg gcccttttta
     1021 tagagtctta tacactagcg gaccctgccg gtagaccaac ccgcaggcgc gtcagtttgc
     1081 tccttccatc aatgcgtcgt agaaacgact tactccttct tgagcagctc cttgaccttg
     1141 ttggcaacaa gtctccgacc tcggaggtgg aggaagagcc tccgatatcg gcggtagtga
     1201 taccagcctc gacggactcc ttgacggcag cctcaacagc gtcaccggcg ggcttcatgt
     1261 taagagagaa cttgagcatc atggcggcag acagaatggt ggcaatgggg ttgaccttct
     1321 gcttgccgag atcgggggca gatccgtgac agggctcgta cagaccgaac gcctcgttgg
     1381 tgtcgggcag agaagccaga gaggcggagg gcagcagacc cagagaaccg gggatgacgg
     1441 aggcctcgtc ggagatgata tcgccaaaca tgttggtggt gatgatgata ccattcatct
     1501 tggagggctg cttgatgagg atcatggcgg ccgagtcgat cagctggtgg ttgagctcga
     1561 gctgggggaa ttcgtccttg aggactcgag tgacagtctt tcgccaaagt cgagaggagg
     1621 ccagcacgtt ggccttgtca agagaccaca cgggaagagg ggggttgtgc tgaagggcca
     1681 ggaaggcggc cattcgggca attcgctcaa cctcaggaac ggagtaggtc tcggtgtcgg
     1741 aagcgacgcc agatccgtca tcctcctttc gctctccaaa gtagatacct ccgacgagct
     1801 ctcggacaat gatgaagtcg gtgccctcaa cgtttcggat gggggagaga tcggcgagct
     1861 tgggcgacag cagctggcag ggtcgcaggt tggcgtacag gttcaggtcc tttcgcagct
     1921 tgaggagacc ctgctcgggt cgcacgtcgg ttcgtccgtc gggagtggtc catacggtgt
     1981 tggcagcgcc tccgacagca ccgagcataa tagagtcagc ctttcggcag atgtcgagag
     2041 tagcgtcggt gatgggctcg ccctccttct caatggcagc tcctccaatg agtcggtcct
     2101 caaacacaaa ctcggtgccg gaggcctcag caacagactt gagcaccttg acggcctcgg
     2161 caatcacctc ggggccacag aagtcgccgc cgagaagaac aatcttcttg gagtcagtct
     2221 tggtcttctt agtttcgggt tccattgtgg atgtgtgtgg ttgtatgtgt gatgtggtgt
     2281 gtggagtgaa aatctgtggc tggcaaacgc tcttgtatat atacgcactt ttgcccgtgc
     2341 tatgtggaag actaaacctc cgaagattgt gactcaggta gtgcggtatc ggctagggac
     2401 ccaaaccttg tcgatgccga tagcgctatc gaacgtaccc cagccggccg ggagtatgtc
     2461 ggaggggaca tacgagatcg tcaagggttt gtggccaact ggtaaataaa tgatgactca
     2521 ggcgacgacg gaattcgaca gcaactactc ctttcaccaa ccatgtgcat tttagctcga
     2581 ataacattca caggcttggt gatctacatc catggtgtct ggccgattac cgtggtgttt
     2641 tggcagtaac gagaatattg agtgaactct tcccatcacc aataaagact catactacaa
     2701 tcacgagcgc ttcagctgcc actatagtgt tggtgacaca atacccctcg atgctgggca
     2761 ttactgtagc aagagatatt catttcatgg cgcattttcc agtctacctg actttttagt
     2821 gccgatttct tctccacatt ttacgctcag tgtgaaaagt tggagtgcac acttaattat
     2881 cgccggtttt cggaaaagta ctatgtgctc aaggttgcac cccacgttac gtatgcagca
     2941 cattgagcag cctttggacc gtggagataa cggtgtggag atagcaacgg gtggatcctc
     3001 tagactgcag ggattaccgc aacacgctat aataaaccaa ttatgttatg tctgataaaa
     3061 ggatgtaaca taggcaagct gctcgtgagt gttgagtacg aaccttagat ccaaatcacc
     3121 cgcaccctac ggatatactt gcttgaatat acagtagtat ttgtaataag ttcataatta
     3181 atatcttacc aaattaggta atctgcaaaa gttcagactg tgaaatgtaa cattttatat
     3241 atcaagctct atttaatgcc tcacagtagt taacataaag acagctgctg ggcctcatgg
     3301 gccttccttt cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat
     3361 ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg
     3421 cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca
     3481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4261 ggtctgacag ttattagaaa aattcatcca gcagacgata aaacgcaata cgctggctat
     4321 ccggtgccgc aatgccatac agcaccagaa aacgatccgc ccattcgccg cccagttctt
     4381 ccgcaatatc acgggtggcc agcgcaatat cctgataacg atccgccacg cccagacggc
     4441 cgcaatcaat aaagccgcta aaacggccat tttccaccat aatgttcggc aggcacgcat
     4501 caccatgggt caccaccaga tcttcgccat ccggcatgct cgctttcaga cgcgcaaaca
     4561 gctctgccgg tgccaggccc tgatgttctt catccagatc atcctgatcc accaggcccg
     4621 cttccatacg ggtacgcgca cgttcaatac gatgtttcgc ctgatgatca aacggacagg
     4681 tcgccgggtc cagggtatgc agacgacgca tggcatccgc cataatgctc actttttctg
     4741 ccggcgccag atggctagac agcagatcct gacccggcac ttcgcccagc agcagccaat
     4801 cacggcccgc ttcggtcacc acatccagca ccgccgcaca cggaacaccg gtggtggcca
     4861 gccagctcag acgcgccgct tcatcctgca gctcgttcag cgcaccgctc agatcggttt
     4921 tcacaaacag caccggacga ccctgcgcgc tcagacgaaa caccgccgca tcagagcagc
     4981 caatggtctg ctgcgcccaa tcatagccaa acagacgttc cacccacgct gccgggctac
     5041 ccgcatgcag gccatcctgt tcaatcatac tcttcctttt tcaatattat tgaagcattt
     5101 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5161 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     5221 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     5281 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg gccgctacag
     5341 ggcgctccca ttcgccattc aggctgcgca actgttggga agggcgtttc ggtgcgggcc
     5401 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     5461 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgacgtaata
     5521 cgactcacta tagggcgaat tgaaggaagg ccgtcaaggc cgcatcagct ggaattcaag
     5581 gagtttggcg cccgtttttt cgagccccac acgtttcggt gagtatgagc ggcggcagat
     5641 tcgagcgttt ccggtttccg cggctggacg agagcccatg atgggggctc ccaccaccag
     5701 caatcagggc cctgattaca cacccacctg taatgtcatg ctgttcatcg tggttaatgc
     5761 tgctgtgtgc tgtgtgtgtg tgttgtttgg cgctcattgt tgcgttatgc agcgtacacc
     5821 acaatattgg aagcttactc tctacaaaca cgccgacgct acgttggcaa ctgcgtgttt
     5881 gtatggacgt tgcgttgcta cagaagaaac atacgaaagc tgatctcatg gtgtcaatca
     5941 tcattcatga attcgttaat aacttagtcg taattagttc agagaaggca taggcgcatg
     6001 cagaatctgg gtgacaaact tcttgccgta gcggttgacg gaggctacag caagaacatt
     6061 gtgcttgata gatgaggtgg ccaaatggtt caaaacaaca tggttggggg caggtagcac
     6121 agagctgtca tccttctcgt tggagttggt gttgagaatc accgtatcaa gatgaggagg
     6181 tagatgtgga ggcactgcct ggctctcggg aatgtcgagt tcggccacgt ccagcgtcag
     6241 gaacatactg gggatctcgt tggtgtactc cagtggtggg gggatgggag tctcgtcctc
     6301 gtattgcgtg attccgtcgt cgccatggct gtggtggtta gatgatgcat ggtgtgtggt
     6361 ttggggctga gtggcagacg tggtttgggg cttcttgagg tcgccctctc ggcaaagagg
     6421 aatgttctct tctgagaacg atgcagggac attgtcagag tcatcgttac cgacatccag
     6481 atagttgacg aggaagccag acgagtctgt ggccgtacca atgtcgtcag cacacttgag
     6541 ctctccgtca atcacaaacc ggaaccgatg agtgccttct gggagatcca gggtgacgga
     6601 aaaggtgccg tctgactggc gatccatagg cagcatcttt ctccagccgg tgaaggtgcc
     6661 tgtgatgtat gccttgttgc ctccttgctt gtagcagatt ttggttctca cagcaccagg
     6721 ccgaggtcca tgtccactgc tactgctcac atcatcatgg ccctggtacc ggagctcaac
     6781 ctcatcgccc gagtcgaccc gcgacagcgg ctttgagacg gcgtcttgtc ccaccacctc
     6841 aatgttcatg ttggcgacat cggtgcctag cttgagctgt ttgaggccgt aggtgaggtt
     6901 gtttttgtag tagtagtctt ctgagtcgtc ttcgtcgtct tctgcgggcg agtcatctcg
     6961 cagctggatc ttggagttga caatgttgcg attatctgag tctcgtcggt ttccctggag
     7021 accctcggcg acttgggctc ccacgtggag cttaccgaag ggcgctgcct cagagggctc
     7081 caggtatttg ggcgagtctt ctggcgtcag aatggaaggg atttctgtcg caacaaccac
     7141 cggagctttt gcggtggggc caatatcggc gtccttttcg tcgtcaggag acacggtgac
     7201 aggcttgatg taagtaaccc gagcagagca gggctgggcc ggggtaacaa gtcggttcat
     7261 gagagctgat ttctcctgaa aagactttgt ggggatgggt actgcggggt gcgcagaggc
     7321 gggtctctgt gttcgtttgg ggcgagagga ggctttgatg gcggcggaat gtgtttgtgt
     7381 ttgagtctgt gccggaagcg actgggtgct ggtttgggag ctttcgctgc ccgtggaccg
     7441 ctgcttgcgc cggggtttgg attctgcgtt tcccatggtc gaaggggcga gtgttgaggc
     7501 agaagtcaag gtctctgggt gctatagata ttccaagagt atgttaaact tgatccaaaa
     7561 gagattagaa tgatgctggg gtcatgtcag agtgctggaa ccttgtcctt ctccacacca
     7621 agtctgagct taaagacaga aagtcaacct ctggtacact ctacgttttg tccttacagt
     7681 ggggtaaaag catttttgtg gacaacggac aggtgtctga ataaagatgt cgaatttggt
     7741 ttacagcatt agtggcatat cttgttatca aaatgacaat ttatcaaggg gttaatggtt
     7801 tggacagaca tcaactgcgg aggggagggg gcgtgagagt tgatgttctc tctgcaccgg
     7861 tttaaacacc caaactgacg actgatttac attccgtctt tcctaacaaa gtgcaccaca
     7921 cgacccagtc ggaccactta atatttcttt tcgccgtaac cctttttttt ctatcttttt
     7981 tttttttaac tctcttgcca caagatgcac atcgctgccg ctttcggcaa ccacactgca
     8041 actcccatct cccacgcagc cacatccaca gcaatcttgg atgtatttgt ggaaagagtt
     8101 gatgggggca cccgagagaa acttgtcgtg ctgggtacat ggtttccccc ccattttgaa
     8161 ctcaaggacc ctggatcagc taccattgtt gccccaaact ggccaatcaa ctcgagccaa
     8221 caatagccct ttgtatgcac gttaaattgt cttatttgct cgatacaagg gagagcatca
     8281 gataggagac agggtaacaa tggcaacaat agaacctgtt ttgaggctga aacagaggct
     8341 gcggccgctg acgtcaactt ggacagtgtg tgagaacgct accaacgcca aagaccagtg
     8401 ccaaactatt ctcaaccgct ctagagagac aaaagtcatc agtggaacgc cacatcctat
     8461 taagccaaat ccgtgaaact ccttgctcac tttacccttt gctttttctc tgcctctgcc
     8521 tccagtgtct cacatgccgg tagctattgt tgctcggtgg tgcaggggga tgaccagcta
     8581 aacccgatga gatagacacc cacaagacgt caactaaagc gtcatgtaag gacgagtatt
     8641 aggggtagag ccaagaacaa aaaaagagac gcatataaat acgagccata caaggagccg
     8701 tacaaggaag gtcaatacgt ctacagtaca acaacaccac ccccacatac agtgcaacaa
     8761 gcagtacgag gaagtacaac aagcagtgca acaggcagta caagaagcac acggcatatc
     8821 tcgtatgtac tcgctcttgt agccttctta cacacgtgaa gtccacttta tcgaggttag
     8881 ttgttttaat gtggggaaat agcaaattta gtcgtcgaga aagggtccta aaacacacac
     8941 cccatagctc tactcatctc atacgtatca taacaaagaa ccagcgaagg gaagcttatt
     9001 agcctttcta ttttttcgtt tgcaaggctt aacaacattg ctgtggagag ggatggggat
     9061 atggaggccg ctggagggag tcggagaggc gttttggagc ggcttggcct ggcgcccagc
     9121 tcgcgaaacg cacctaggac cctttggcac gccgaaatgt gccacttttc agtctagtaa
     9181 cgccttacct acgtcattcc atgcatgcat gtttgcgcct tttttccctt gcccttgatc
     9241 gccacacagt acagtgcact gtacagtgga ggttttgggg gggtcttaga tgggagctaa
     9301 aagcggccta gcggtacact agtgggattg tatggagtgg catggagcct aggtggagcc
     9361 tgacaggacg cacgaccggc tagcccgtga cagacgatgg gtggctcctg ttgtccaccg
     9421 cgtacaaatg tttgggccaa agtcttgtca gccttgcttg cgaacctaat tcccaatttt
     9481 gtcacttcgc acccccattg atcgagccct aacccctgcc catcaggcaa tccaattaag
     9541 ctcgcattgt ctgccttgtt tagtttggct cctgcccgtt tcggcgtcca cttgcacaaa
     9601 cacaaacaag cattatatat aaggctcgtc tctccctccc aaccacactc acttttttgc
     9661 ccgtcttccc ttgctaacac aaaagtcaag aacacaaaca accaccccaa cccccttaca
     9721 cacaagacat atctacagcg gtaccccc
//
