LOCUS       40924_48123        8200 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Neurospora expression vector pYFPN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Bardiya N, Alexander WG, Perdue TD, Barry EG, Metzenberg RL, Pukkila
            PJ, Shiu PK.
  TITLE     Characterization of interactions between and among components of the
            meiotic silencing by unpaired DNA machinery in Neurospora crassa 
            using bimolecular fluorescence complementation
  JOURNAL   Genetics 178 (1), 593-596 (2008)
  PUBMED    18202398
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Shiu PKT.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2007) Division of Biological Sciences, University 
            of Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65203, USA
REFERENCE   3  (bases 1 to 8200)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8200)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2008"; volume: "178"; issue: "1"; pages: "593-596"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2007) Division of Biological Sciences, University of 
            Missouri-Columbia, 103 Tucker Hall, Columbia, MO 65203, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5028..5492
                     /codon_start=1
                     /label=VN155(I152L)
                     /note="improved N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     promoter        complement(5520..5538)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(8199..8200,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg
     4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag
     4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc
     4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc
     4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt
     4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag
     4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt
     4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca
     4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg
     4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt
     4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac
     4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg
     4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt
     4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc
     4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct
     4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa
     4981 tctagaacta gtggatcccc gggttaatta accgctccat cgccacgatg gtgagcaagg
     5041 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5101 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5161 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     5221 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     5281 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5341 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5401 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5461 actacaacag ccacaacgtc tatatcatgg cctaagggcc cggtacccag cttttgttcc
     5521 ctttagtgag ggttaattag ggcgtggacg gctaatgggg tctgaatgct aaaagacacc
     5581 atttcccaca ctccctctga ttttttttat ggtttttttt tatcttcatt tttcgccgtc
     5641 ttttattcgg ttgtttggga ggttcacatg caacgtgcaa gactgcaagt tttttgagat
     5701 gggcgcttca tgcaatgcag catcactgca gggacattgt gacatccacg tttcgatcgt
     5761 ctgtgcttcc cccgttcatg tacgcatgat ctgaaaaaaa aaaaaaaaaa aaaggttgat
     5821 gtaataatga aaataaacac ccagggacga gcacctcatc tgctggaata gggagaaaaa
     5881 ctcaagggcc gtttgggtct ccacacttac caacagattc atgtactgat cttcagcctg
     5941 tacagaacag tacaatgtcc aaaactcgtt atcattactc tatgtgccca aaatgaccca
     6001 gatatacatc aacccactac atccttttgt tcttgttttt tttttttttt tttgctactt
     6061 cgaaaagaat ttccgtttag ttgctcttgc tcatgtgctc aagacggatg ctgaccgccc
     6121 ttctgtgagc ctcgagctcc tcaaccttag ccaactgcat gacagcctgg ccgacgtttt
     6181 tgagaccctc ggcagtcaag ttggagctgg taatgtgctt gacgaacgag gcgaaattga
     6241 cgccagagta ctgcttgcca aagccatagg tagctatgtg tcttgacgtt agcagaagaa
     6301 tctgtgaagc ggggctattt atgatatgta acttacgcag cgagtggtta acaccagcag
     6361 agtaatcgcc aacggactca ggagtccagg cgccaatgaa gacactaccg gcgttcatga
     6421 caagatcgac agccttctcg gcctccttga tctggaggat caagtgctca ggagcgtact
     6481 tgttgctgag ctccatggcc tcctcgacgg tcttgacctg cacggtgatc gagtgggcga
     6541 tggagccacg gacaatctgg acgcgaggaa gctccgtagc ctgacggtga acctcgtcct
     6601 caatagcctg aagatgctcc tcgtcgaggt caatagcgat caggatgacc tgactgtcaa
     6661 cgccgtgctc agcctgggac aggagatccg aggcaacgaa cgcggggttg gcgtccttgt
     6721 cagcgatgac cagcacctcg gacgggccag cgggcatgtc aataccaacg gcagcgttgg
     6781 tgtcgttgct gacgaacatc ttggcagcag tgacgaactg gttaccgggg ccgagaatct
     6841 tgtcgacctt ggtgatgctc tcggtgccgt aggccatggc agctacggcc tgggcaccgc
     6901 cggcaagcac gatggactcg gccccaacct tgtgagcgac gtggacaatc tcgggagtga
     6961 tggttccgtc ggcgcgggga ggagaggcga acacaatctt gttgcagccg gcgaccatgg
     7021 cgggaacacc cagcataagg gcagtgctgg ggagaacggc ggtaccgccg gggatgtagc
     7081 agccgacggc ctcgatggga cgagagaaac ggctgcagac aacaccgggc atggtctcga
     7141 cctggagggg cttctcctcc ttctgggcgg cgtggaactt gcggatgttc tcgaaggaca
     7201 cgtcgatggc agcaatggtc tcctcaggga gctgcataag ctccttgggg aagggcgcct
     7261 tcaggacggg gctagtaaga gaggtagcct tctcgaactt gtgagtgtac gacagaacag
     7321 ccttgtcgcc gttcttgcgg acgtcctcga tgatggggac aatgatcttg tagatggcat
     7381 cggacgactt ttgcgcagga cgcttgagag cagcatcgag ctcctcggta gagaccttgg
     7441 aggcgtcgaa acgtctcatg gtgatcttct caggggtggt ctcctgggcg gcctccttgg
     7501 tgacaggggc cgaagtggca gcgagagcgg acgccgcagg cttgatgccc tccttctcag
     7561 cccacttacc cttagcatct ccagtcctgc gcttgacctt ccagctcttg gcgtcaaggc
     7621 tcctttcgat atcggcaaga gtaacgccgg cagcaacggc cctggtaaga gcaaagtaga
     7681 agagatcggc agcctcaaag gcgatttcct ggggggtctg agcggtgcag agctcctcag
     7741 cctcctccat gatcttggcc cggactagct tctcatcgga gaagagacgg gcagtgtagg
     7801 agccctcggg ggcagactgt ttcctcgaaa tcaaagtctg ctcgagcttg ggaaggcctt
     7861 tgagctgacc aaagcagccg gactgatcga ggtggcagaa acgacccttc tgcttcacga
     7921 caaacttgag agcatcgtta tcgcagtcaa gcgagatgcg gacgagctcc tgagtgtctc
     7981 cggaagtagc acccttgtac cagagaccgc gcttccggct ctgatagaca ccagtctgtg
     8041 tcctgagggc ctcgttcaca ctctcggcac tgctgtagac cagacccaga gcagtgtcgt
     8101 gctcatcaac gacaacggtg gggagaagac catcgggacg gtcagacttc cagacgctcg
     8161 agaggatggt ggagatggca agcttcagct gctcgagttc
//
